Calcium in PDB 6gez: The Structure of Twitch-2B N532F

The structure of The Structure of Twitch-2B N532F, PDB code: 6gez was solved by P.Trigo Mourino, M.Paulat, T.Thestrup, O.Griesbeck, C.Griesinger, S.Becker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	115.34 / 2.47
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	57.619, 157.472, 169.419, 90.00, 90.00, 90.00
R / Rfree (%)	20.7 / 23.4

The binding sites of Calcium atom in the The Structure of Twitch-2B N532F (pdb code 6gez). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the The Structure of Twitch-2B N532F, PDB code: 6gez:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the The Structure of Twitch-2B N532F


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The Structure of Twitch-2B N532F within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca601 b:60.4 occ:1.00 OD1 A:ASP248 2.2 62.2 1.0 O A:PHE254 2.2 70.8 1.0 O A:HOH711 2.3 48.0 1.0 OD1 A:ASP250 2.5 63.6 1.0 OD1 A:ASN252 2.5 67.2 1.0 OE1 A:GLU259 2.6 72.0 1.0 OE2 A:GLU259 2.7 70.0 1.0 CD A:GLU259 3.0 69.3 1.0 CG A:ASN252 3.3 68.6 1.0 CG A:ASP250 3.4 65.4 1.0 CG A:ASP248 3.4 62.5 1.0 C A:PHE254 3.4 71.1 1.0 OD2 A:ASP250 3.7 69.2 1.0 ND2 A:ASN252 4.0 70.5 1.0 N A:ASN252 4.1 64.6 1.0 N A:PHE254 4.1 71.7 1.0 CA A:ASP248 4.1 60.7 1.0 CB A:ASP248 4.2 61.8 1.0 CB A:ASN252 4.3 68.3 1.0 OD2 A:ASP248 4.3 64.9 1.0 CA A:PHE254 4.4 73.5 1.0 N A:ILE255 4.4 69.4 1.0 C A:ASP248 4.4 60.3 1.0 CA A:ILE255 4.4 66.1 1.0 N A:ASP250 4.5 60.1 1.0 CA A:ASN252 4.5 67.9 1.0 CG A:GLU259 4.5 68.0 1.0 CB A:ASP250 4.6 64.2 1.0 N A:ASP256 4.6 64.4 1.0 N A:GLY253 4.6 68.5 1.0 N A:GLY251 4.7 61.9 1.0 O A:ASP248 4.7 64.1 1.0 CA A:ASP250 4.7 63.1 1.0 C A:ASP250 4.8 62.8 1.0 C A:ASN252 4.8 68.5 1.0 N A:PHE249 4.8 59.6 1.0 C A:ILE255 5.0 65.3 1.0

Calcium binding site 2 out of 4 in the The Structure of Twitch-2B N532F


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The Structure of Twitch-2B N532F within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca602 b:85.4 occ:1.00 OD1 A:ASN288 2.3 81.0 1.0 O A:HOH725 2.3 73.6 1.0 OD1 A:ASP284 2.3 82.5 1.0 O A:ARG290 2.4 76.1 1.0 OE1 A:GLU295 2.5 76.1 1.0 OE2 A:GLU295 2.7 82.4 1.0 OD1 A:ASP286 2.8 94.0 1.0 CD A:GLU295 3.0 77.1 1.0 CG A:ASN288 3.2 83.5 1.0 CG A:ASP284 3.5 81.4 1.0 CG A:ASP286 3.5 94.0 1.0 C A:ARG290 3.6 76.4 1.0 OD2 A:ASP286 3.7 96.1 1.0 ND2 A:ASN288 3.7 82.4 1.0 CA A:ASP284 4.1 79.0 1.0 N A:ASN288 4.1 86.6 1.0 CB A:ASP284 4.2 79.8 1.0 N A:ARG290 4.3 81.4 1.0 CB A:ASN288 4.3 85.7 1.0 N A:ASP286 4.4 89.0 1.0 OD2 A:ASP284 4.4 80.3 1.0 C A:ASP284 4.4 81.8 1.0 CA A:ILE291 4.5 74.0 1.0 N A:ILE291 4.5 74.4 1.0 N A:ASP292 4.5 72.3 1.0 CG A:GLU295 4.5 76.0 1.0 N A:LYS287 4.5 87.5 1.0 CA A:ARG290 4.5 79.2 1.0 OD2 A:ASP292 4.5 82.1 1.0 N A:THR285 4.6 82.9 1.0 CA A:ASN288 4.7 86.7 1.0 CB A:ASP286 4.7 92.8 1.0 C A:ASP286 4.7 90.6 1.0 CA A:ASP286 4.7 91.5 1.0 CG A:ARG290 4.8 86.1 1.0 N A:GLY289 4.9 83.8 1.0 C A:ASN288 5.0 85.5 1.0 O A:ASP284 5.0 81.1 1.0 C A:ILE291 5.0 71.5 1.0

Calcium binding site 3 out of 4 in the The Structure of Twitch-2B N532F


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of The Structure of Twitch-2B N532F within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca601 b:69.4 occ:1.00 O B:PHE254 2.2 73.1 1.0 O B:HOH706 2.2 58.3 1.0 OD1 B:ASP248 2.3 61.7 1.0 OD1 B:ASP250 2.5 63.7 1.0 OD1 B:ASN252 2.5 69.1 1.0 OE1 B:GLU259 2.5 73.8 1.0 OE2 B:GLU259 2.7 73.5 1.0 CD B:GLU259 3.0 74.1 1.0 CG B:ASN252 3.3 69.2 1.0 CG B:ASP250 3.4 66.0 1.0 C B:PHE254 3.4 76.1 1.0 CG B:ASP248 3.5 62.5 1.0 OD2 B:ASP250 3.7 70.8 1.0 ND2 B:ASN252 3.9 70.6 1.0 N B:PHE254 4.1 75.4 1.0 N B:ASN252 4.2 63.7 1.0 CA B:ASP248 4.2 62.6 1.0 N B:ILE255 4.3 75.9 1.0 CB B:ASP248 4.3 63.2 1.0 CB B:ASN252 4.3 67.7 1.0 CA B:ILE255 4.3 74.0 1.0 CA B:PHE254 4.3 79.2 1.0 OD2 B:ASP248 4.4 63.8 1.0 N B:ASP256 4.4 75.2 1.0 CG B:GLU259 4.5 73.3 1.0 C B:ASP248 4.5 62.0 1.0 N B:ASP250 4.6 61.7 1.0 CA B:ASN252 4.6 66.2 1.0 CB B:ASP250 4.6 63.8 1.0 N B:GLY253 4.7 68.8 1.0 N B:GLY251 4.8 58.7 1.0 C B:ASN252 4.8 68.4 1.0 O B:ASP248 4.8 61.6 1.0 CA B:ASP250 4.8 61.8 1.0 C B:ASP250 4.8 60.5 1.0 C B:ILE255 4.9 75.1 1.0 OD2 B:ASP256 4.9 80.7 1.0 N B:PHE249 4.9 63.5 1.0

Calcium binding site 4 out of 4 in the The Structure of Twitch-2B N532F


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of The Structure of Twitch-2B N532F within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca602 b:88.8 occ:1.00 O B:HOH710 2.2 62.3 1.0 OD1 B:ASN288 2.3 88.5 1.0 OD1 B:ASP284 2.3 88.8 1.0 O B:ARG290 2.4 85.8 1.0 OE1 B:GLU295 2.5 79.1 1.0 OE2 B:GLU295 2.7 81.7 1.0 OD1 B:ASP286 2.8 91.5 1.0 CD B:GLU295 2.9 79.1 1.0 CG B:ASN288 3.2 92.9 1.0 CG B:ASP286 3.4 93.0 1.0 CG B:ASP284 3.5 86.7 1.0 C B:ARG290 3.6 85.6 1.0 OD2 B:ASP286 3.7 92.3 1.0 ND2 B:ASN288 3.7 92.2 1.0 CA B:ASP284 4.1 82.8 1.0 N B:ASN288 4.2 97.7 1.0 CB B:ASP284 4.2 84.3 1.0 N B:ASP286 4.3 92.1 1.0 CB B:ASN288 4.4 95.5 1.0 C B:ASP284 4.4 86.8 1.0 N B:ARG290 4.4 87.7 1.0 OD2 B:ASP284 4.4 86.2 1.0 CG B:GLU295 4.4 77.2 1.0 N B:ASP292 4.5 77.9 1.0 OD2 B:ASP292 4.5 84.0 1.0 N B:LYS287 4.5 93.3 1.0 CA B:ILE291 4.5 79.1 1.0 N B:ILE291 4.5 80.7 1.0 N B:THR285 4.6 87.9 1.0 CA B:ARG290 4.6 87.8 1.0 CB B:ASP286 4.6 94.5 1.0 CA B:ASN288 4.7 96.9 1.0 C B:ASP286 4.7 94.0 1.0 CA B:ASP286 4.7 94.2 1.0 CG B:ARG290 4.9 94.4 1.0 O B:ASP284 4.9 89.0 1.0 N B:GLY289 5.0 91.6 1.0

P.Trigo-Mourino, T.Thestrup, O.Griesbeck, C.Griesinger, S.Becker. Dynamic Tuning of Fret in A Green Fluorescent Protein Biosensor. Sci Adv V. 5 W4988 2019.
ISSN: ESSN 2375-2548
PubMed: 31457088
DOI: 10.1126/SCIADV.AAW4988