Calcium in PDB 6gsp: Ribonuclease T1/3'-Gmp, 15 Weeks

Enzymatic activity of Ribonuclease T1/3'-Gmp, 15 Weeks

All present enzymatic activity of Ribonuclease T1/3'-Gmp, 15 Weeks:
3.1.27.3;

Protein crystallography data

The structure of Ribonuclease T1/3'-Gmp, 15 Weeks, PDB code: 6gsp was solved by I.Zegers, L.Wyns, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 15.00 / 2.20
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 48.350, 51.110, 40.540, 90.00, 90.00, 90.00
R / Rfree (%) 19.5 / 21.7

Calcium Binding Sites:

The binding sites of Calcium atom in the Ribonuclease T1/3'-Gmp, 15 Weeks (pdb code 6gsp). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Ribonuclease T1/3'-Gmp, 15 Weeks, PDB code: 6gsp:

Calcium binding site 1 out of 1 in 6gsp

Go back to Calcium Binding Sites List in 6gsp
Calcium binding site 1 out of 1 in the Ribonuclease T1/3'-Gmp, 15 Weeks


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ribonuclease T1/3'-Gmp, 15 Weeks within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca107

b:87.9
occ:1.00
OD2 A:ASP15 2.2 15.0 1.0
O A:HOH130 2.4 28.4 1.0
OD1 A:ASP15 2.6 15.1 1.0
CG A:ASP15 2.7 13.6 1.0
O A:HOH143 3.2 34.6 1.0
O A:HOH141 3.3 33.7 1.0
CB A:ASP15 4.2 9.8 1.0
OG A:SER12 4.2 12.7 1.0
O A:HOH167 4.3 41.4 1.0
O A:CYS10 4.6 12.1 1.0
N A:SER12 4.7 15.2 1.0
CB A:SER12 4.9 15.0 1.0

Reference:

I.Zegers, R.Loris, G.Dehollander, A.Fattah Haikal, F.Poortmans, J.Steyaert, L.Wyns. Hydrolysis of A Slow Cyclic Thiophosphate Substrate of Rnase T1 Analyzed By Time-Resolved Crystallography. Nat.Struct.Biol. V. 5 280 1998.
ISSN: ISSN 1072-8368
PubMed: 9546218
DOI: 10.1038/NSB0498-280
Page generated: Sat Dec 12 06:08:33 2020

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