Calcium in PDB 6hk5: X-Ray Structure of A Truncated Mutant of the Metallochaperone Cooj with A High-Affinity Nickel-Binding Site

The structure of X-Ray Structure of A Truncated Mutant of the Metallochaperone Cooj with A High-Affinity Nickel-Binding Site, PDB code: 6hk5 was solved by M.Alfano, J.Perard, C.Basset, P.Carpentier, B.Zambelli, J.Timm, S.Crouzy, S.Ciurli, C.Cavazza, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.75 / 2.04
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	46.800, 74.280, 70.640, 90.00, 92.74, 90.00
R / Rfree (%)	20 / 25.9

The structure of X-Ray Structure of A Truncated Mutant of the Metallochaperone Cooj with A High-Affinity Nickel-Binding Site also contains other interesting chemical elements:

Nickel	(Ni)	4 atoms
Chlorine	(Cl)	3 atoms

The binding sites of Calcium atom in the X-Ray Structure of A Truncated Mutant of the Metallochaperone Cooj with A High-Affinity Nickel-Binding Site (pdb code 6hk5). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the X-Ray Structure of A Truncated Mutant of the Metallochaperone Cooj with A High-Affinity Nickel-Binding Site, PDB code: 6hk5:

Calcium binding site 1 out of 1 in the X-Ray Structure of A Truncated Mutant of the Metallochaperone Cooj with A High-Affinity Nickel-Binding Site


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of X-Ray Structure of A Truncated Mutant of the Metallochaperone Cooj with A High-Affinity Nickel-Binding Site within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca101 b:42.6 occ:1.00 O C:HOH223 2.4 32.5 1.0 OE2 G:GLU24 2.4 36.7 1.0 O D:HOH223 2.4 35.9 1.0 OD2 C:ASP21 2.6 34.2 1.0 OD1 C:ASP21 2.6 34.3 1.0 O C:HOH221 2.7 32.4 1.0 O G:HOH137 2.8 38.8 1.0 CG C:ASP21 2.9 35.4 1.0 CD G:GLU24 3.5 41.9 1.0 O D:HOH217 3.8 39.9 1.0 O G:HOH108 3.8 33.8 1.0 CG G:GLU24 3.9 34.9 1.0 O D:HOH236 4.2 39.5 1.0 OD1 D:ASN62 4.3 34.6 1.0 O C:ALA17 4.4 24.6 1.0 CB C:ASP21 4.5 32.5 1.0 OE1 G:GLU24 4.5 39.9 1.0 O D:HOH241 4.7 37.9 1.0 O D:HOH215 4.7 47.6 1.0 O C:HOH201 4.8 28.9 1.0 O C:HOH205 4.8 37.2 1.0 C C:ALA17 4.9 26.2 1.0

M.Alfano, J.Perard, P.Carpentier, C.Basset, B.Zambelli, J.Timm, S.Crouzy, S.Ciurli, C.Cavazza. The Carbon Monoxide Dehydrogenase Accessory Protein Cooj Is A Histidine-Rich Multidomain Dimer Containing An Unexpected Ni(II)-Binding Site. J.Biol.Chem. V. 294 7601 2019.
ISSN: ESSN 1083-351X
PubMed: 30858174
DOI: 10.1074/JBC.RA119.008011