Calcium in PDB 6iss: Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239

Enzymatic activity of Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239

All present enzymatic activity of Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239:
1.11.1.14;

Protein crystallography data

The structure of Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239, PDB code: 6iss was solved by H.Seo, H.Son, K.-J.Kim, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 29.49 / 1.53
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 72.959, 93.740, 227.371, 90.00, 90.00, 90.00
R / Rfree (%) 17 / 19.6

Other elements in 6iss:

The structure of Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239 also contains other interesting chemical elements:

Iron (Fe) 2 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239 (pdb code 6iss). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239, PDB code: 6iss:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in 6iss

Go back to Calcium Binding Sites List in 6iss
Calcium binding site 1 out of 4 in the Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca402

b:12.1
occ:1.00
 OD1 A:ASP48 2.3 11.3 1.0
O A:HOH623 2.3 13.9 1.0
O A:GLY66 2.3 11.8 1.0
OD1 A:ASP68 2.4 12.3 1.0
OG A:SER70 2.4 12.5 1.0
O A:ASP48 2.5 12.3 1.0
O A:HOH567 2.5 13.6 1.0
C A:ASP48 3.4 11.5 1.0
CG A:ASP48 3.4 13.0 1.0
CG A:ASP68 3.5 14.9 1.0
CB A:SER70 3.5 14.1 1.0
C A:GLY66 3.6 11.6 1.0
CA A:ASP48 3.8 11.6 1.0
N A:SER70 3.9 13.5 1.0
OD2 A:ASP68 3.9 14.5 1.0
N A:ASP68 4.0 12.0 1.0
CB A:ASP48 4.2 11.6 1.0
O A:HOH553 4.2 14.6 1.0
CA A:SER70 4.3 13.8 1.0
OD2 A:ASP48 4.3 12.4 1.0
CA A:GLY66 4.3 13.1 1.0
N A:GLY66 4.3 13.8 1.0
N A:ILE71 4.4 14.0 1.0
CB A:ALA51 4.5 15.1 1.0
O A:ALA51 4.5 14.3 1.0
N A:GLY69 4.5 11.6 1.0
N A:CYS49 4.5 11.5 1.0
N A:CYS67 4.6 13.0 1.0
OE2 A:GLU78 4.6 14.8 1.0
CB A:ASP68 4.7 13.0 1.0
OE1 A:GLU78 4.8 14.1 1.0
CA A:ASP68 4.8 13.2 1.0
CA A:CYS67 4.8 12.3 1.0
C A:SER70 4.8 13.9 1.0
O A:HIS47 4.9 12.2 1.0
C A:GLY65 4.9 14.5 1.0
C A:CYS67 4.9 12.7 1.0
C A:ASP68 4.9 11.3 1.0
C A:GLY69 4.9 11.5 1.0

Calcium binding site 2 out of 4 in 6iss

Go back to Calcium Binding Sites List in 6iss
Calcium binding site 2 out of 4 in the Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca403

b:8.3
occ:1.00
 O A:THR196 2.4 9.8 1.0
O A:ILE199 2.4 9.4 1.0
O A:SER177 2.4 8.1 1.0
OD1 A:ASP201 2.4 9.3 1.0
OG A:SER177 2.5 8.2 1.0
OG1 A:THR196 2.5 9.7 1.0
OD2 A:ASP194 2.5 8.4 1.0
OD1 A:ASP194 2.7 9.1 1.0
CG A:ASP194 3.0 8.8 1.0
C A:THR196 3.2 9.0 1.0
CG A:ASP201 3.3 10.2 1.0
C A:SER177 3.4 8.9 1.0
CB A:THR196 3.6 9.3 1.0
CB A:SER177 3.6 8.4 1.0
C A:ILE199 3.6 9.0 1.0
CA A:SER177 3.7 8.4 1.0
OD2 A:ASP201 3.7 11.0 1.0
CA A:THR196 3.9 9.0 1.0
N A:ASP201 4.1 9.6 1.0
N A:PRO197 4.2 9.1 1.0
N A:THR196 4.3 9.5 1.0
CA A:ILE199 4.4 9.9 1.0
N A:ILE199 4.4 9.8 1.0
CA A:PRO197 4.5 9.0 1.0
CB A:ASP194 4.5 9.3 1.0
CB A:ILE199 4.5 11.0 1.0
CB A:ASP201 4.6 9.9 1.0
O A:ASP201 4.6 9.9 1.0
N A:VAL178 4.6 9.1 1.0
O A:HOH694 4.6 9.7 1.0
CB A:GLN203 4.6 9.7 1.0
N A:PHE200 4.6 8.9 1.0
CA A:ASP201 4.7 9.6 1.0
CA A:PHE200 4.8 8.9 1.0
C A:ASP201 4.8 9.7 1.0
CG1 A:VAL178 4.8 9.6 1.0
CG2 A:THR196 4.9 9.6 1.0
C A:PHE200 4.9 9.3 1.0
C A:PRO197 5.0 8.2 1.0

Calcium binding site 3 out of 4 in 6iss

Go back to Calcium Binding Sites List in 6iss
Calcium binding site 3 out of 4 in the Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239 within 5.0Å range:
probe atom residue distance (Å) B Occ
G:Ca402

b:15.4
occ:1.00
 OD1 G:ASP48 2.2 16.3 1.0
O G:HOH625 2.3 17.6 1.0
OG G:SER70 2.4 16.0 1.0
OD1 G:ASP68 2.4 18.5 1.0
O G:GLY66 2.4 15.6 1.0
O G:ASP48 2.4 15.1 1.0
O G:HOH579 2.5 16.2 1.0
CG G:ASP48 3.4 15.6 1.0
C G:ASP48 3.4 13.8 1.0
CG G:ASP68 3.4 18.0 1.0
CB G:SER70 3.5 17.6 1.0
C G:GLY66 3.6 15.5 1.0
CA G:ASP48 3.8 13.3 1.0
OD2 G:ASP68 3.9 17.0 1.0
N G:SER70 3.9 17.1 1.0
N G:ASP68 4.1 16.9 1.0
CB G:ASP48 4.2 13.9 1.0
CA G:SER70 4.2 16.9 1.0
OD2 G:ASP48 4.2 14.9 1.0
O G:HOH553 4.3 17.7 1.0
N G:GLY66 4.3 18.5 1.0
CA G:GLY66 4.4 16.6 1.0
O G:ALA51 4.4 18.2 1.0
N G:ILE71 4.5 16.1 1.0
CB G:ALA51 4.5 15.3 1.0
N G:CYS49 4.6 13.0 1.0
OE2 G:GLU78 4.6 19.1 1.0
CB G:ASP68 4.6 17.8 1.0
N G:CYS67 4.7 14.5 1.0
N G:GLY69 4.7 16.8 1.0
OE1 G:GLU78 4.7 18.6 1.0
C G:SER70 4.8 17.2 1.0
CA G:ASP68 4.8 16.7 1.0
CA G:CYS67 4.8 15.0 1.0
O G:HIS47 4.9 14.0 1.0
C G:GLY65 4.9 18.1 1.0
C G:ASP68 5.0 19.1 1.0
C G:GLY69 5.0 14.8 1.0

Calcium binding site 4 out of 4 in 6iss

Go back to Calcium Binding Sites List in 6iss
Calcium binding site 4 out of 4 in the Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Lignin Peroxidase H8 Triple Mutant S49C/A67C/H239 within 5.0Å range:
probe atom residue distance (Å) B Occ
G:Ca403

b:8.1
occ:1.00
 O G:SER177 2.4 7.3 1.0
O G:THR196 2.4 7.8 1.0
OD2 G:ASP194 2.4 7.6 1.0
O G:ILE199 2.4 8.7 1.0
OD1 G:ASP201 2.5 8.0 1.0
OG G:SER177 2.5 7.2 1.0
OG1 G:THR196 2.5 8.7 1.0
OD1 G:ASP194 2.7 7.6 1.0
CG G:ASP194 2.9 7.8 1.0
C G:THR196 3.3 8.6 1.0
C G:SER177 3.4 8.0 1.0
CG G:ASP201 3.4 7.9 1.0
CB G:THR196 3.6 8.1 1.0
CB G:SER177 3.6 7.7 1.0
CA G:SER177 3.6 7.3 1.0
C G:ILE199 3.7 9.4 1.0
OD2 G:ASP201 3.8 8.6 1.0
CA G:THR196 3.8 8.1 1.0
N G:ASP201 4.1 8.5 1.0
N G:PRO197 4.2 8.7 1.0
N G:THR196 4.3 7.7 1.0
CB G:ASP194 4.4 7.4 1.0
N G:ILE199 4.4 9.1 1.0
CA G:PRO197 4.4 8.5 1.0
CA G:ILE199 4.5 9.2 1.0
O G:ASP201 4.5 7.3 1.0
O G:HOH698 4.5 9.1 1.0
N G:VAL178 4.6 8.2 1.0
CB G:ASP201 4.6 8.4 1.0
CB G:ILE199 4.6 10.0 1.0
N G:PHE200 4.7 8.4 1.0
CB G:GLN203 4.7 8.1 1.0
CA G:ASP201 4.8 8.1 1.0
CG1 G:VAL178 4.8 9.0 1.0
CA G:PHE200 4.8 8.5 1.0
C G:ASP201 4.8 8.1 1.0
CG2 G:THR196 4.9 8.6 1.0
C G:PHE200 4.9 8.5 1.0
C G:PRO197 5.0 8.6 1.0

Reference:

H.Son, H.Seo, L.T.M.Pham, S.H.Kang, K.-J.Kim, Y.H.Kim. Reinforced Helix-Loop Interaction of Lignin Peroxidase H8 of Phanerochaete Chrysosporium (LIPH8) For Improving Acid-pH Stability As Well As Thermostability To Be Published.
Page generated: Sat Dec 12 06:11:54 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy