Calcium in PDB 6jd9: Proteus Mirabilis Lipase Mutant - I118V/E130G

All present enzymatic activity of Proteus Mirabilis Lipase Mutant - I118V/E130G:
3.1.1.3;

The structure of Proteus Mirabilis Lipase Mutant - I118V/E130G, PDB code: 6jd9 was solved by B.S.Heater, W.S.Chan, M.K.Chan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.85 / 1.58
Space group	P 32
Cell size a, b, c (Å), α, β, γ (°)	65.310, 65.310, 63.591, 90.00, 90.00, 120.00
R / Rfree (%)	13.9 / 16.3

The binding sites of Calcium atom in the Proteus Mirabilis Lipase Mutant - I118V/E130G (pdb code 6jd9). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Proteus Mirabilis Lipase Mutant - I118V/E130G, PDB code: 6jd9:

Calcium binding site 1 out of 1 in the Proteus Mirabilis Lipase Mutant - I118V/E130G


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Proteus Mirabilis Lipase Mutant - I118V/E130G within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca301 b:9.6 occ:1.00 O A:LEU264 2.3 10.9 1.0 OD1 A:ASP256 2.3 10.2 1.0 OD2 A:ASP213 2.3 11.1 1.0 OD1 A:ASN210 2.4 12.7 1.0 O A:HOH423 2.4 10.9 1.0 O A:GLN260 2.4 9.7 1.0 C A:GLN260 3.3 9.7 1.0 CG A:ASN210 3.4 12.1 1.0 C A:LEU264 3.4 11.8 1.0 CG A:ASP213 3.5 11.0 1.0 CG A:ASP256 3.5 10.9 1.0 N A:VAL261 3.6 9.7 1.0 ND2 A:ASN210 3.8 12.1 1.0 CA A:VAL265 4.0 11.1 1.0 CB A:ASP213 4.0 11.4 1.0 N A:VAL265 4.1 11.0 1.0 OD2 A:ASP256 4.2 9.7 1.0 CA A:ASP256 4.3 8.8 1.0 OG1 A:THR215 4.4 10.9 1.0 OD1 A:ASP213 4.4 11.8 1.0 CB A:ASP256 4.5 9.3 1.0 OD2 A:ASP253 4.5 8.7 1.0 N A:ASP256 4.5 8.8 1.0 CA A:LEU264 4.6 12.4 1.0 CB A:LEU264 4.6 13.0 1.0 OD1 A:ASP253 4.7 9.1 1.0 CG1 A:VAL265 4.7 11.9 1.0 CA A:GLN260 4.8 8.8 1.0 CB A:ASN210 4.8 11.8 1.0 C A:VAL265 4.9 15.3 1.0 CB A:VAL265 4.9 11.4 1.0 CB A:HIS216 4.9 9.2 1.0 CG1 A:ILE255 4.9 14.9 1.0 CG A:ASP253 5.0 9.1 1.0

B.S.Heater, W.S.Chan, M.M.Lee, M.K.Chan. Directed Evolution of A Genetically Encoded Immobilized Lipase For the Efficient Production of Biodiesel From Waste Cooking Oil. Biotechnol Biofuels V. 12 165 2019.
ISSN: ESSN 1754-6834
PubMed: 31297153
DOI: 10.1186/S13068-019-1509-5