Calcium in PDB 6lp1: Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei.

All present enzymatic activity of Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei.:
2.8.3.5;

The structure of Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei., PDB code: 6lp1 was solved by K.Mochizuki, D.K.Inaoka, T.Shiba, K.Fukuda, H.Kurasawa, M.Mazet, Y.Millerioux, F.Bringaud, M.Boshart, E.O.Balogun, S.Harada, K.Hirayama, K.Kita, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.97 / 2.01
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	59.886, 164.635, 187.573, 90.00, 90.00, 90.00
R / Rfree (%)	22.5 / 27.9

The binding sites of Calcium atom in the Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei. (pdb code 6lp1). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei., PDB code: 6lp1:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei. within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca501 b:40.7 occ:1.00 OD2 A:ASP239 2.5 22.3 1.0 O A:GLN211 2.5 18.8 1.0 OD1 A:ASP239 2.9 22.9 1.0 CG A:ASP239 3.0 22.6 1.0 C A:GLN211 3.6 22.3 1.0 O A:HOH626 4.1 16.9 1.0 O A:GLY210 4.3 19.4 1.0 N A:THR212 4.5 21.8 1.0 CA A:THR212 4.5 21.2 1.0 CG1 A:VAL213 4.5 24.2 1.0 CA A:GLN211 4.5 24.2 1.0 CB A:ASP239 4.5 22.0 1.0 N A:VAL213 4.7 21.2 1.0 O A:HOH675 4.8 25.1 1.0 O B:HOH673 4.8 28.7 1.0 O A:TYR237 4.8 19.4 1.0

Calcium binding site 2 out of 4 in the Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei. within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca501 b:64.3 occ:1.00 O B:GLN211 2.6 23.5 1.0 OD2 B:ASP239 2.9 24.7 1.0 OD1 B:ASP239 3.3 24.7 1.0 CG B:ASP239 3.5 25.1 1.0 O A:HOH693 3.6 23.7 1.0 O A:HOH648 3.6 20.2 1.0 C B:GLN211 3.7 24.6 1.0 O B:GLY210 4.0 22.8 1.0 O B:HOH647 4.4 19.1 1.0 CG1 B:VAL213 4.5 26.8 1.0 O B:TYR237 4.6 18.8 1.0 CA B:GLN211 4.6 26.7 1.0 N B:THR212 4.6 22.8 1.0 CA B:THR212 4.6 22.7 1.0 CB B:ASP239 5.0 23.9 1.0 N B:VAL213 5.0 23.3 1.0 C B:GLY210 5.0 24.5 1.0

Calcium binding site 3 out of 4 in the Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei. within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca501 b:47.8 occ:1.00 O C:GLN211 2.6 27.2 1.0 OD1 C:ASP239 2.6 24.6 1.0 OD2 C:ASP239 2.7 23.4 1.0 CG C:ASP239 3.0 23.4 1.0 C C:GLN211 3.8 25.7 1.0 O C:GLY210 4.5 21.1 1.0 CB C:ASP239 4.5 21.3 1.0 CA C:THR212 4.6 23.4 1.0 N C:THR212 4.6 24.4 1.0 O C:TYR237 4.6 20.8 1.0 CG1 C:VAL213 4.6 24.5 1.0 CA C:GLN211 4.7 26.9 1.0 N C:VAL213 4.8 24.5 1.0

Calcium binding site 4 out of 4 in the Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of Acetate:Succinate Coa Transferase (Asct) From Trypanosoma Brucei. within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca501 b:39.8 occ:1.00 OD1 D:ASP239 2.5 18.9 1.0 O D:GLN211 2.5 22.3 1.0 OD2 D:ASP239 2.6 19.8 1.0 CG D:ASP239 2.9 19.6 1.0 C D:GLN211 3.6 23.5 1.0 O D:HOH619 4.0 14.1 1.0 O D:GLY210 4.2 23.4 1.0 CB D:ASP239 4.4 20.3 1.0 CA D:THR212 4.5 21.0 1.0 N D:THR212 4.5 21.5 1.0 N D:VAL213 4.6 19.5 1.0 CA D:GLN211 4.6 24.7 1.0 O D:HOH653 4.7 24.2 1.0 CG2 D:VAL213 4.8 19.0 1.0 O D:TYR237 4.8 18.2 1.0 O D:HOH611 4.9 36.8 1.0 C D:THR212 5.0 20.8 1.0

K.Mochizuki, D.K.Inaoka, M.Mazet, T.Shiba, K.Fukuda, H.Kurasawa, Y.Millerioux, M.Boshart, E.O.Balogun, S.Harada, K.Hirayama, F.Bringaud, K.Kita. The Asct/Scs Cycle Fuels Mitochondrial Atp and Acetate Production in Trypanosoma Brucei. Biochim Biophys Acta V.1861 48283 2020BIOENERG.
ISSN: ISSN 1879-2650
PubMed: 32763239
DOI: 10.1016/J.BBABIO.2020.148283