Calcium in PDB 6m6p: Structure of Marine Bacterial Laminarinase Mutant E135A in Complex with 1,3-Beta-Cellotriosyl-Glucose

The structure of Structure of Marine Bacterial Laminarinase Mutant E135A in Complex with 1,3-Beta-Cellotriosyl-Glucose, PDB code: 6m6p was solved by J.Yang, Y.Xu, M.Tanokura, L.Long, T.Miyakawa, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.90 / 2.27
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	66.998, 95.644, 72.989, 90.00, 90.00, 90.00
R / Rfree (%)	17.4 / 22.8

The binding sites of Calcium atom in the Structure of Marine Bacterial Laminarinase Mutant E135A in Complex with 1,3-Beta-Cellotriosyl-Glucose (pdb code 6m6p). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Structure of Marine Bacterial Laminarinase Mutant E135A in Complex with 1,3-Beta-Cellotriosyl-Glucose, PDB code: 6m6p:

Calcium binding site 1 out of 1 in the Structure of Marine Bacterial Laminarinase Mutant E135A in Complex with 1,3-Beta-Cellotriosyl-Glucose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of Marine Bacterial Laminarinase Mutant E135A in Complex with 1,3-Beta-Cellotriosyl-Glucose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca301 b:57.3 occ:1.00 O A:ASP245 2.2 33.0 1.0 O A:GLY68 2.3 42.2 1.0 O A:GLU30 2.4 38.8 1.0 OD1 A:ASP245 2.6 37.5 1.0 O A:HOH452 2.7 32.0 1.0 C A:ASP245 3.3 33.9 1.0 C A:GLY68 3.5 39.1 1.0 C A:GLU30 3.6 37.2 1.0 CG A:ASP245 3.7 36.3 1.0 CA A:ASP245 3.8 33.1 1.0 CA A:GLU30 4.1 37.8 1.0 CA A:GLY68 4.1 38.9 1.0 CB A:GLU30 4.3 37.4 1.0 CB A:ASP245 4.4 35.2 1.0 N A:TYR246 4.4 34.7 1.0 N A:ASN69 4.5 37.5 1.0 CB A:TYR246 4.6 33.5 1.0 OD2 A:ASP245 4.6 37.7 1.0 N A:PHE31 4.7 35.2 1.0 OE1 A:GLU30 4.7 38.5 1.0 CB A:PHE31 4.7 35.9 1.0 ND2 A:ASN69 4.7 35.2 1.0 CA A:TYR246 4.7 33.1 1.0 CG2 A:THR32 4.8 46.0 1.0 CA A:ASN69 4.8 37.1 1.0 CD2 A:PHE31 4.9 33.3 1.0 C A:PHE31 5.0 39.0 1.0

J.Yang, Y.Xu, T.Miyakawa, L.Long, M.Tanokura. Molecular Basis For Substrate Recognition and Catalysis By A Marine Bacterial Laminarinase. Appl.Environ.Microbiol. V. 86 2020.
ISSN: ESSN 1098-5336
PubMed: 32917756
DOI: 10.1128/AEM.01796-20