Atomistry » Calcium » PDB 6m4m-6mr8 » 6ml1
Atomistry »
  Calcium »
    PDB 6m4m-6mr8 »
      6ml1 »

Calcium in PDB 6ml1: Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv

Enzymatic activity of Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv

All present enzymatic activity of Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv:
3.4.19.12;

Protein crystallography data

The structure of Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv, PDB code: 6ml1 was solved by A.U.Singer, J.Teyra, G.Boehmelt, M.Lenter, F.Sicheri, S.S.Sidhu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.32 / 1.90
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 42.770, 115.290, 95.320, 90.00, 91.92, 90.00
R / Rfree (%) 18.9 / 22

Other elements in 6ml1:

The structure of Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv also contains other interesting chemical elements:

Zinc (Zn) 2 atoms
Chlorine (Cl) 2 atoms
Sodium (Na) 4 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv (pdb code 6ml1). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv, PDB code: 6ml1:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in 6ml1

Go back to Calcium Binding Sites List in 6ml1
Calcium binding site 1 out of 4 in the Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca1001

b:19.0
occ:1.00
 OD1 A:ASP858 2.4 19.6 1.0
O B:HOH1137 2.4 20.6 1.0
O A:HOH1134 2.4 17.7 1.0
OD1 B:ASP858 2.4 20.1 1.0
OE1 A:GLN933 2.5 19.8 1.0
OE1 B:GLN933 2.5 20.9 1.0
OD2 A:ASP858 2.6 24.1 1.0
OD2 B:ASP858 2.8 25.1 1.0
CG A:ASP858 2.8 21.7 1.0
CG B:ASP858 2.9 24.1 1.0
CD B:GLN933 3.5 22.2 1.0
CD A:GLN933 3.5 21.5 1.0
NE2 B:GLN933 3.8 17.8 1.0
NE2 A:GLN933 3.8 19.4 1.0
OD1 A:ASN876 4.3 17.8 1.0
OD1 B:ASN876 4.3 17.1 1.0
CB A:ASP858 4.3 21.5 1.0
CB B:ASP858 4.4 21.1 1.0
ND2 B:ASN876 4.5 21.9 1.0
CD B:ARG874 4.5 23.7 1.0
ND2 A:ASN876 4.5 20.0 1.0
CD A:ARG874 4.6 23.4 1.0
CL B:CL1004 4.6 47.3 0.6
O A:HOH1238 4.7 45.9 1.0
CG B:GLN933 4.8 26.9 1.0
CG A:GLN933 4.8 21.3 1.0
CG B:ASN876 4.8 18.8 1.0
CG A:ASN876 4.9 20.0 1.0
N A:ASP858 4.9 18.8 1.0
N B:ASP858 5.0 19.0 1.0
CA A:ASP858 5.0 17.5 1.0

Calcium binding site 2 out of 4 in 6ml1

Go back to Calcium Binding Sites List in 6ml1
Calcium binding site 2 out of 4 in the Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca1002

b:31.3
occ:1.00
 O A:HOH1233 2.3 46.9 1.0
NE2 G:HIS14 2.3 46.3 1.0
O G:HOH103 2.4 27.2 1.0
SG A:CYS873 2.4 35.8 1.0
CD2 G:HIS14 2.9 43.1 1.0
CB A:CYS873 3.4 31.0 1.0
CE1 G:HIS14 3.5 52.4 1.0
CA A:CYS873 3.7 28.6 1.0
CD A:ARG932 3.8 35.7 1.0
NH1 A:ARG932 4.0 35.8 1.0
O A:GLY872 4.1 34.7 1.0
N A:CYS873 4.2 33.6 1.0
CG G:HIS14 4.2 46.7 1.0
CB G:ALA12 4.3 51.0 1.0
NE A:ARG932 4.3 34.5 1.0
C A:GLY872 4.4 39.4 1.0
CZ A:ARG932 4.4 38.6 1.0
ND1 G:HIS14 4.5 51.5 1.0
CG A:ARG932 4.9 35.0 1.0

Calcium binding site 3 out of 4 in 6ml1

Go back to Calcium Binding Sites List in 6ml1
Calcium binding site 3 out of 4 in the Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca1001

b:62.0
occ:1.00
 OE1 B:GLU450 2.3 56.3 1.0
OE1 B:GLU814 2.6 70.7 1.0
CD B:GLU450 2.9 61.4 1.0
OE2 B:GLU450 3.0 56.2 1.0
CD B:GLU814 3.3 71.2 1.0
OE2 B:GLU814 3.5 70.0 1.0
NE2 B:GLN816 3.7 45.5 1.0
CG B:GLU450 4.2 56.9 1.0
O B:HOH1122 4.5 55.3 1.0
SG B:CYS812 4.5 41.3 1.0
CB B:CYS812 4.6 46.5 1.0
CG B:GLU814 4.7 59.1 1.0
CD B:GLN816 4.8 57.3 1.0
CB B:GLU450 4.8 48.8 1.0
CB B:GLU814 4.9 52.6 1.0

Calcium binding site 4 out of 4 in 6ml1

Go back to Calcium Binding Sites List in 6ml1
Calcium binding site 4 out of 4 in the Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Structure of the USP15 Deubiquitinase Domain in Complex with An Affinity-Matured Inhibitory Ubv within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca101

b:62.7
occ:1.00
 O C:LYS33 2.6 49.3 1.0
C C:LYS33 3.8 53.4 1.0
OE2 C:GLU34 3.9 60.1 1.0
CA C:GLU34 4.4 48.0 1.0
CD C:GLU34 4.5 55.1 1.0
O C:HOH203 4.6 40.0 1.0
N C:GLU34 4.6 44.3 1.0
CA C:LYS33 4.9 46.1 1.0

Reference:

J.Teyra, A.U.Singer, F.W.Schmitges, P.Jaynes, S.Kit Leng Lui, M.J.Polyak, N.Fodil, J.R.Krieger, J.Tong, C.Schwerdtfeger, B.B.Brasher, D.F.J.Ceccarelli, J.Moffat, F.Sicheri, M.F.Moran, P.Gros, P.J.A.Eichhorn, M.Lenter, G.Boehmelt, S.S.Sidhu. Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15. Structure V. 27 590 2019.
ISSN: ISSN 1878-4186
PubMed: 30713027
DOI: 10.1016/J.STR.2019.01.002
Page generated: Tue Jul 16 11:24:00 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy