Calcium in PDB 6nvy: Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans

The structure of Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans, PDB code: 6nvy was solved by W.Blankenfeldt, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.25 / 2.27
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	172.996, 172.996, 209.566, 90.00, 90.00, 120.00
R / Rfree (%)	17.2 / 20

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 12;

Binding sites:

The binding sites of Calcium atom in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans (pdb code 6nvy). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 12 binding sites of Calcium where determined in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans, PDB code: 6nvy:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Calcium binding site 1 out of 12 in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca301 b:76.0 occ:1.00 O A:HOH404 2.3 64.5 1.0 OE2 A:GLU53 2.4 73.0 1.0 OD1 A:ASP136 2.4 68.2 1.0 O A:HOH428 2.4 79.0 1.0 O A:HOH417 2.4 56.9 1.0 O A:HOH456 2.5 58.3 1.0 CG A:ASP136 3.3 69.3 1.0 OD2 A:ASP136 3.5 70.9 1.0 CD A:GLU53 3.5 70.4 1.0 HD2 A:ARG49 3.5 72.3 1.0 HH11 A:ARG49 4.0 79.7 1.0 OE1 A:GLU53 4.0 77.3 1.0 HE A:ARG139 4.2 69.5 1.0 HD2 A:ARG139 4.4 65.1 1.0 OD2 A:ASP78 4.4 77.7 1.0 CD A:ARG49 4.5 60.2 1.0 NH1 A:ARG49 4.5 66.4 1.0 OD1 A:ASP78 4.6 74.3 1.0 CB A:ASP136 4.7 64.6 1.0 OD2 A:ASP133 4.7 69.6 1.0 CG A:GLU53 4.7 66.7 1.0 HA A:ASP136 4.8 76.3 1.0 HG2 A:GLU53 4.8 80.0 1.0 HH12 A:ARG49 4.9 79.7 1.0 OD1 A:ASP133 4.9 65.5 1.0 OG1 A:THR135 4.9 62.0 1.0 NE A:ARG139 4.9 57.9 1.0 HD3 A:ARG49 4.9 72.3 1.0 HB3 A:ASP136 4.9 77.5 1.0 CG A:ASP78 4.9 77.5 1.0 HG3 A:GLU53 5.0 80.0 1.0 HG3 A:ARG49 5.0 69.2 1.0 HG1 A:THR135 5.0 74.4 1.0

Calcium binding site 2 out of 12 in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca601 b:36.6 occ:1.00 OE1 A:GLU154 2.3 44.4 1.0 OD1 B:ASN73 2.3 35.7 1.0 O B:THR75 2.3 42.0 1.0 OD1 B:ASP76 2.4 43.9 1.0 O B:HOH751 2.4 33.0 1.0 OE2 B:GLU256 2.5 44.0 1.0 OE1 B:GLU256 2.5 41.5 1.0 CD B:GLU256 2.8 42.2 1.0 HA B:ASP76 3.0 48.7 1.0 HD21 B:ASN73 3.0 49.3 1.0 CG B:ASN73 3.2 36.8 1.0 C B:THR75 3.4 45.0 1.0 ND2 B:ASN73 3.4 41.1 1.0 CD A:GLU154 3.5 48.5 1.0 CG B:ASP76 3.5 38.9 1.0 HH21 B:ARG203 3.7 48.1 1.0 CA B:ASP76 3.8 40.6 1.0 HG3 A:GLU154 3.9 48.5 1.0 N B:ASP76 4.0 42.6 1.0 H B:THR75 4.0 52.8 1.0 NH2 B:ARG203 4.2 40.1 1.0 CB B:ASP76 4.2 43.5 1.0 CG A:GLU154 4.2 40.4 1.0 H B:GLU256 4.3 37.9 1.0 O B:GLU209 4.3 46.5 1.0 HD22 B:ASN73 4.3 49.3 1.0 CG B:GLU256 4.3 34.6 1.0 OE2 A:GLU154 4.4 45.6 1.0 HE B:ARG203 4.4 44.5 1.0 OD2 B:ASP76 4.4 45.8 1.0 HH22 B:ARG203 4.5 48.1 1.0 HG2 A:GLU154 4.5 48.5 1.0 HA B:ASN73 4.6 52.2 1.0 CB B:ASN73 4.6 42.0 1.0 O B:HOH940 4.6 34.1 1.0 HG3 B:GLU256 4.6 41.6 1.0 N B:THR75 4.6 44.0 1.0 CA B:THR75 4.6 44.7 1.0 O A:HOH413 4.6 34.4 1.0 HB3 B:ASP76 4.7 52.2 1.0 HB3 B:GLU256 4.7 38.5 1.0 O B:HOH704 4.7 60.1 1.0 H B:ILE77 4.7 48.2 1.0 HD21 A:ASN151 4.7 62.3 1.0 HA3 B:GLY255 4.8 45.9 1.0 H B:ASP76 4.8 51.2 1.0 HG2 B:GLU256 4.8 41.6 1.0 CZ B:ARG203 4.9 39.8 1.0 HB B:THR75 4.9 54.0 1.0 NE B:ARG203 4.9 37.0 1.0 HB2 B:ASN73 4.9 50.5 1.0 CA B:ASN73 4.9 43.5 1.0 HB3 B:GLU209 5.0 60.8 1.0 HB2 B:ASP76 5.0 52.2 1.0

Calcium binding site 3 out of 12 in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca602 b:47.1 occ:1.00 O B:LEU342 2.2 51.6 1.0 OD1 B:ASP336 2.3 46.7 1.0 O B:HOH875 2.3 40.2 1.0 OD1 B:ASN338 2.4 64.6 1.0 OD1 B:ASP340 2.4 46.8 1.0 OD1 B:ASP344 2.4 42.9 1.0 CG B:ASP340 3.1 52.8 1.0 HA B:ASP336 3.2 53.2 1.0 H B:LEU342 3.2 64.8 1.0 C B:LEU342 3.3 46.0 1.0 CG B:ASP336 3.4 52.0 1.0 H B:ASP340 3.4 65.6 1.0 OD2 B:ASP340 3.4 50.0 1.0 HB2 B:LEU342 3.4 61.1 1.0 CG B:ASN338 3.4 59.4 1.0 HD21 B:ASN338 3.4 70.4 1.0 HA B:ASP344 3.6 56.2 1.0 CG B:ASP344 3.6 42.3 1.0 ND2 B:ASN338 3.8 58.7 1.0 H B:ASN338 3.8 59.7 1.0 N B:LEU342 3.9 54.0 1.0 CA B:ASP336 3.9 44.4 1.0 CA B:LEU342 4.0 46.1 1.0 CB B:LEU342 4.1 50.9 1.0 CB B:ASP336 4.1 46.1 1.0 N B:ASP344 4.1 43.6 1.0 CA B:ASP344 4.2 46.8 1.0 H B:LYS339 4.2 69.5 1.0 N B:ASP340 4.2 54.7 1.0 OD2 B:ASP336 4.2 51.9 1.0 C B:TYR343 4.3 49.1 1.0 HB2 B:ASP336 4.3 55.4 1.0 HB3 B:LEU342 4.3 61.1 1.0 H B:ASP344 4.4 52.3 1.0 CB B:ASP340 4.4 52.5 1.0 N B:TYR343 4.4 47.2 1.0 HA B:TYR343 4.4 53.6 1.0 C B:ASP336 4.5 49.7 1.0 OD2 B:ASP344 4.5 42.9 1.0 CB B:ASP344 4.5 45.9 1.0 H B:LYS337 4.5 59.1 1.0 HB3 B:ASP340 4.6 63.0 1.0 N B:LYS339 4.6 57.9 1.0 N B:ASN338 4.6 49.8 1.0 H B:GLY341 4.6 60.7 1.0 O B:HOH863 4.6 49.2 1.0 CA B:TYR343 4.6 44.6 1.0 O B:TYR343 4.6 44.2 1.0 O B:GLU335 4.7 45.5 1.0 HD22 B:ASN338 4.7 70.4 1.0 N B:LYS337 4.7 49.3 1.0 CA B:ASP340 4.7 53.9 1.0 CB B:ASN338 4.8 55.4 1.0 N B:GLY341 4.8 50.6 1.0 HB3 B:ASP344 4.9 55.1 1.0 HA B:LEU342 4.9 55.3 1.0 C B:ASP340 4.9 53.2 1.0 C B:ASN338 5.0 54.0 1.0 HB3 B:ASP336 5.0 55.4 1.0

Calcium binding site 4 out of 12 in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca603 b:61.7 occ:1.00 OE1 B:GLU8 2.4 72.7 1.0 O B:HOH925 2.4 63.2 1.0 O B:ARG189 2.5 61.5 1.0 OE2 B:GLU8 2.9 71.2 1.0 CD B:GLU8 3.0 70.6 1.0 CA B:CA605 3.7 0.1 1.0 C B:ARG189 3.7 59.8 1.0 HA3 B:GLY190 3.9 60.9 1.0 HA B:ARG189 4.2 64.8 1.0 HB3 B:ARG189 4.3 67.7 1.0 O B:HOH912 4.3 52.6 1.0 CG B:GLU8 4.4 63.6 1.0 CA B:ARG189 4.5 54.0 1.0 N B:GLY190 4.6 52.8 1.0 HB3 B:GLU8 4.7 65.1 1.0 CA B:GLY190 4.7 50.8 1.0 HG2 B:GLU8 4.8 76.3 1.0 HB2 B:GLU8 4.9 65.1 1.0 CB B:ARG189 4.9 56.5 1.0 HG3 B:GLU8 5.0 76.3 1.0 CB B:GLU8 5.0 54.3 1.0

Calcium binding site 5 out of 12 in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca604 b:70.2 occ:1.00 OD1 B:ASP284 2.3 42.3 1.0 O B:HOH865 2.5 54.0 1.0 O B:HOH784 2.5 50.0 1.0 O B:HOH732 2.5 73.8 1.0 CG B:ASP284 3.3 41.0 1.0 HZ1 B:LYS12 3.3 79.5 1.0 HZ2 B:LYS12 3.4 79.5 1.0 OD2 B:ASP284 3.6 45.4 1.0 NZ B:LYS12 3.6 66.2 1.0 HZ3 B:LYS12 3.6 79.5 1.0 OE1 B:GLU287 3.7 51.0 1.0 HE B:ARG277 3.7 47.2 1.0 OE2 B:GLU287 3.9 59.7 1.0 HA B:ASP284 4.2 50.1 1.0 CD B:GLU287 4.2 55.4 1.0 HG2 B:GLU283 4.2 74.7 1.0 OE2 B:GLU283 4.3 75.9 1.0 NE B:ARG277 4.5 39.4 1.0 CB B:ASP284 4.6 40.9 1.0 HD3 B:ARG277 4.7 45.0 1.0 HH21 B:ARG277 4.7 52.0 1.0 CA B:ASP284 4.8 41.8 1.0 OD1 B:ASP281 4.8 43.1 1.0 N B:ASP284 5.0 40.7 1.0 H B:ASP284 5.0 48.8 1.0

Calcium binding site 6 out of 12 in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca605 b:0.1 occ:1.00 O B:GLY14 2.4 52.7 1.0 O B:HOH912 2.5 52.6 1.0 O B:HOH950 2.5 55.2 1.0 OE1 B:GLU8 2.5 72.7 1.0 O B:HOH761 2.7 51.0 1.0 HB2 B:GLU8 3.1 65.1 1.0 C B:GLY14 3.4 47.0 1.0 HB3 B:GLU8 3.5 65.1 1.0 CA B:CA603 3.7 61.7 1.0 CD B:GLU8 3.7 70.6 1.0 CB B:GLU8 3.7 54.3 1.0 HA3 B:GLY14 3.8 51.4 1.0 HA B:ASN15 3.9 57.1 1.0 CA B:GLY14 4.1 42.8 1.0 HA3 B:GLY190 4.3 60.9 1.0 HA2 B:GLY14 4.3 51.4 1.0 CG B:GLU8 4.3 63.6 1.0 O B:ARG189 4.3 61.5 1.0 OD1 B:ASN15 4.4 51.7 1.0 N B:ASN15 4.4 43.3 1.0 CA B:ASN15 4.7 47.6 1.0 OE2 B:GLU8 4.7 71.2 1.0 HG3 B:GLU8 4.8 76.3 1.0 HA2 B:GLY190 4.9 60.9 1.0 C B:ARG189 4.9 59.8 1.0 CA B:GLY190 4.9 50.8 1.0 HA B:GLU8 4.9 67.3 1.0

Calcium binding site 7 out of 12 in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca301 b:70.9 occ:1.00 OE2 C:GLU53 2.3 74.9 1.0 O C:HOH419 2.4 63.0 1.0 OD1 C:ASP136 2.4 58.0 1.0 O C:HOH412 2.5 65.6 1.0 O C:HOH401 2.5 82.8 1.0 O C:HOH415 2.5 60.9 1.0 O C:HOH454 2.5 60.9 1.0 CG C:ASP136 3.4 55.1 1.0 CD C:GLU53 3.5 71.2 1.0 OD2 C:ASP136 3.6 63.6 1.0 HD2 C:ARG49 3.7 77.9 1.0 OE1 C:GLU53 4.0 75.6 1.0 HE C:ARG139 4.1 74.5 1.0 OD2 C:ASP78 4.2 79.4 1.0 HH11 C:ARG49 4.3 82.8 1.0 HD2 C:ARG139 4.3 63.3 1.0 OD2 C:ASP133 4.4 80.9 1.0 OD1 C:ASP78 4.5 72.2 1.0 HG2 C:GLU53 4.6 80.6 1.0 CG C:GLU53 4.6 67.2 1.0 OG1 C:THR135 4.6 62.7 1.0 CD C:ARG49 4.7 64.9 1.0 HA C:ASP136 4.7 70.8 1.0 CB C:ASP136 4.7 53.4 1.0 CG C:ASP78 4.8 75.5 1.0 NE C:ARG139 4.8 62.0 1.0 HG1 C:THR135 4.8 75.2 1.0 NH1 C:ARG49 4.9 69.0 1.0 HG3 C:GLU53 4.9 80.6 1.0 HD3 C:ARG49 5.0 77.9 1.0 OD1 C:ASP133 5.0 76.3 1.0

Calcium binding site 8 out of 12 in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 8 of Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca601 b:37.8 occ:1.00 OE1 C:GLU154 2.3 50.9 1.0 O D:THR75 2.3 41.3 1.0 OD1 D:ASN73 2.4 40.9 1.0 OD1 D:ASP76 2.4 45.9 1.0 OE2 D:GLU256 2.4 37.1 1.0 O D:HOH739 2.4 39.4 1.0 OE1 D:GLU256 2.5 37.9 1.0 CD D:GLU256 2.8 41.0 1.0 HA D:ASP76 2.9 45.6 1.0 HD21 D:ASN73 3.1 49.1 1.0 CG D:ASN73 3.3 36.7 1.0 C D:THR75 3.4 43.2 1.0 CD C:GLU154 3.5 53.8 1.0 CG D:ASP76 3.5 46.6 1.0 ND2 D:ASN73 3.6 40.9 1.0 HH21 D:ARG203 3.6 50.5 1.0 CA D:ASP76 3.7 38.0 1.0 HG3 C:GLU154 3.9 56.5 1.0 N D:ASP76 3.9 39.2 1.0 H D:THR75 4.1 55.5 1.0 NH2 D:ARG203 4.1 42.1 1.0 CB D:ASP76 4.2 43.1 1.0 H D:GLU256 4.2 43.2 1.0 CG D:GLU256 4.3 39.6 1.0 CG C:GLU154 4.3 47.1 1.0 O D:GLU209 4.3 40.7 1.0 HE D:ARG203 4.3 51.5 1.0 OE2 C:GLU154 4.3 48.1 1.0 OD2 D:ASP76 4.4 47.1 1.0 HD22 D:ASN73 4.4 49.1 1.0 HH22 D:ARG203 4.5 50.5 1.0 HG3 D:GLU256 4.6 47.6 1.0 O C:HOH434 4.6 35.3 1.0 HB3 D:GLU256 4.6 40.7 1.0 HA D:ASN73 4.6 51.9 1.0 O D:HOH924 4.6 42.4 1.0 HG2 C:GLU154 4.6 56.5 1.0 HB3 D:ASP76 4.6 51.7 1.0 H D:ILE77 4.6 47.3 1.0 CA D:THR75 4.6 45.3 1.0 N D:THR75 4.6 46.2 1.0 CB D:ASN73 4.7 38.6 1.0 H D:ASP76 4.8 47.1 1.0 HG2 D:GLU256 4.8 47.6 1.0 HA3 D:GLY255 4.8 57.1 1.0 CZ D:ARG203 4.8 44.9 1.0 NE D:ARG203 4.8 42.9 1.0 HB D:THR75 4.9 59.9 1.0 HB2 D:ASP76 4.9 51.7 1.0 C D:ASP76 4.9 42.4 1.0

Calcium binding site 9 out of 12 in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 9 of Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca602 b:47.6 occ:1.00 O D:LEU342 2.3 44.1 1.0 OD1 D:ASP336 2.3 44.9 1.0 OD1 D:ASP344 2.3 44.1 1.0 OD1 D:ASP340 2.4 57.5 1.0 O D:HOH748 2.4 39.0 1.0 OD1 D:ASN338 2.4 56.2 1.0 CG D:ASP340 3.1 59.8 1.0 HA D:ASP336 3.2 57.7 1.0 H D:LEU342 3.3 56.9 1.0 OD2 D:ASP340 3.4 54.5 1.0 C D:LEU342 3.4 45.9 1.0 CG D:ASP336 3.4 51.7 1.0 H D:ASP340 3.4 69.1 1.0 CG D:ASN338 3.4 55.0 1.0 HB2 D:LEU342 3.5 61.8 1.0 HD21 D:ASN338 3.5 69.0 1.0 CG D:ASP344 3.6 41.5 1.0 HA D:ASP344 3.6 54.2 1.0 H D:ASN338 3.8 58.2 1.0 ND2 D:ASN338 3.9 57.5 1.0 CA D:ASP336 4.0 48.1 1.0 N D:LEU342 4.0 47.4 1.0 CA D:LEU342 4.1 43.3 1.0 N D:ASP344 4.1 44.5 1.0 CB D:ASP336 4.2 44.5 1.0 CA D:ASP344 4.2 45.2 1.0 C D:TYR343 4.2 43.0 1.0 CB D:LEU342 4.2 51.5 1.0 N D:ASP340 4.3 57.6 1.0 OD2 D:ASP336 4.3 50.9 1.0 H D:LYS339 4.3 67.1 1.0 H D:LYS337 4.3 58.1 1.0 HB2 D:ASP336 4.4 53.4 1.0 CB D:ASP340 4.4 54.7 1.0 HA D:TYR343 4.4 46.7 1.0 H D:ASP344 4.4 53.4 1.0 N D:TYR343 4.4 44.5 1.0 OD2 D:ASP344 4.4 45.7 1.0 O D:HOH857 4.5 51.1 1.0 CB D:ASP344 4.5 39.1 1.0 C D:ASP336 4.5 44.2 1.0 N D:ASN338 4.5 48.5 1.0 H D:GLY341 4.6 60.9 1.0 O D:TYR343 4.6 44.1 1.0 HB3 D:ASP340 4.6 65.6 1.0 N D:LYS337 4.6 48.4 1.0 CA D:TYR343 4.6 38.9 1.0 O D:GLU335 4.6 43.7 1.0 HB3 D:LEU342 4.6 61.8 1.0 N D:LYS339 4.7 55.9 1.0 CA D:ASP340 4.7 57.6 1.0 HD22 D:ASN338 4.7 69.0 1.0 CB D:ASN338 4.8 54.9 1.0 N D:GLY341 4.8 50.8 1.0 HB3 D:ASP344 4.9 47.0 1.0 C D:ASP340 4.9 53.5 1.0 C D:ASN338 5.0 52.0 1.0 CA D:ASN338 5.0 51.4 1.0

Calcium binding site 10 out of 12 in the Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 10 of Crystal Structure of Penicillin G Acylase From Bacillus Thermotolerans within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca603 b:54.4 occ:1.00 O D:HOH827 2.2 47.3 1.0 OD1 D:ASP9 2.4 46.6 1.0 OD2 D:ASP9 2.5 42.6 1.0 CG D:ASP9 2.8 37.7 1.0 O D:HOH780 3.3 65.3 1.0 CB D:ASP9 4.3 36.8 1.0 HG3 D:GLU8 4.4 59.8 1.0 HB3 D:ASP9 4.6 44.1 1.0 OE2 D:GLU8 4.7 63.7 1.0 HB2 D:ASP9 4.8 44.1 1.0 O D:HOH706 4.8 47.1 1.0 HA D:ASP9 4.8 40.8 1.0

J.Mayer, J.Pippel, G.Gunther, C.Muller, A.Lauermann, T.Knuuti, W.Blankenfeldt, D.Jahn, R.Biedendieck. Crystal Structures and Protein Engineering of Three Different Penicillin G Acylases From Gram-Positive Bacteria with Different Thermostability. Appl.Microbiol.Biotechnol. V. 103 7537 2019.
ISSN: ESSN 1432-0614
PubMed: 31227867
DOI: 10.1007/S00253-019-09977-8