Atomistry » Calcium » PDB 6o1t-6ojd » 6o44
Atomistry »
  Calcium »
    PDB 6o1t-6ojd »
      6o44 »

Calcium in PDB 6o44: Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis

Enzymatic activity of Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis

All present enzymatic activity of Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis:
3.4.21.62;

Protein crystallography data

The structure of Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis, PDB code: 6o44 was solved by H.Tang, K.Shi, H.Aihara, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 59.16 / 1.83
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 74.177, 80.123, 87.729, 90.00, 90.00, 90.00
R / Rfree (%) 18.7 / 22.2

Calcium Binding Sites:

The binding sites of Calcium atom in the Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis (pdb code 6o44). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis, PDB code: 6o44:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in 6o44

Go back to Calcium Binding Sites List in 6o44
Calcium binding site 1 out of 4 in the Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca301

b:15.9
occ:1.00
 OE1 A:GLN2 2.2 16.7 1.0
O A:LEU75 2.3 19.3 1.0
OD1 A:ASN77 2.3 19.3 1.0
O A:ILE79 2.4 19.3 1.0
OD1 A:ASP41 2.4 23.8 1.0
O A:VAL81 2.4 17.3 1.0
OD2 A:ASP41 2.6 17.1 1.0
CG A:ASP41 2.9 26.2 1.0
CG A:ASN77 3.3 20.8 1.0
CD A:GLN2 3.3 20.4 1.0
HG3 A:GLN2 3.3 25.9 1.0
H A:ASN77 3.4 23.7 1.0
HD21 A:ASN77 3.4 26.3 1.0
C A:LEU75 3.5 18.2 1.0
C A:ILE79 3.6 25.1 1.0
HG13 A:ILE79 3.6 30.0 1.0
C A:VAL81 3.6 19.6 1.0
H A:VAL81 3.6 28.5 1.0
H A:ILE79 3.7 29.7 1.0
ND2 A:ASN77 3.7 21.8 1.0
N A:VAL81 3.8 23.7 1.0
CG A:GLN2 3.8 21.5 1.0
N A:ASN77 3.9 20.1 1.0
HA A:ASN76 4.0 28.5 1.0
HA3 A:GLY80 4.0 21.3 1.0
HB2 A:GLN2 4.1 28.6 1.0
C A:GLY80 4.1 17.7 1.0
HB2 A:LEU75 4.1 21.6 1.0
HA A:LEU82 4.2 27.0 1.0
HB1 A:ALA74 4.2 19.8 1.0
H A:LEU75 4.3 23.0 1.0
N A:LEU75 4.3 19.1 1.0
CA A:VAL81 4.3 17.5 1.0
CA A:LEU75 4.4 26.5 1.0
CA A:GLY80 4.4 18.0 1.0
CB A:ASP41 4.4 17.0 1.0
N A:ILE79 4.4 24.7 1.0
CG1 A:ILE79 4.4 24.9 1.0
N A:ASN76 4.4 18.8 1.0
N A:GLY80 4.4 20.4 1.0
HG12 A:ILE79 4.4 30.0 1.0
NE2 A:GLN2 4.5 18.8 1.0
CB A:ASN77 4.5 20.6 1.0
CA A:ASN76 4.5 23.7 1.0
CB A:GLN2 4.5 23.8 1.0
CA A:ILE79 4.5 25.7 1.0
HD22 A:ASN77 4.6 26.3 1.0
C A:ASN76 4.6 22.3 1.0
CA A:ASN77 4.6 21.8 1.0
HE21 A:GLN2 4.6 22.7 1.0
HG2 A:GLN2 4.6 25.9 1.0
N A:LEU82 4.6 22.7 1.0
HA A:ASP41 4.7 20.4 1.0
C A:ASN77 4.7 24.2 1.0
O A:ASN77 4.7 21.2 1.0
O A:GLY80 4.7 21.1 1.0
CB A:LEU75 4.7 18.3 1.0
HB2 A:ASP41 4.8 20.2 1.0
C A:ALA74 4.8 17.1 1.0
HA A:VAL81 4.8 21.1 1.0
HB3 A:ASP41 4.8 20.2 1.0
CA A:LEU82 4.9 22.4 1.0
HB2 A:LEU82 4.9 26.2 1.0
HB3 A:ASN77 4.9 24.6 1.0
HD13 A:LEU82 5.0 31.8 1.0

Calcium binding site 2 out of 4 in 6o44

Go back to Calcium Binding Sites List in 6o44
Calcium binding site 2 out of 4 in the Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca302

b:37.8
occ:1.00
 NE2 A:HIS64 2.2 25.7 1.0
SG A:CYS221 2.4 33.8 1.0
O1 A:EDO304 2.7 48.2 1.0
HB2 A:CYS221 2.8 24.4 1.0
CB A:CYS221 2.9 20.2 1.0
HB3 A:CYS221 2.9 24.4 1.0
CD2 A:HIS64 3.1 25.0 1.0
HD2 A:HIS64 3.1 30.1 1.0
CE1 A:HIS64 3.3 23.1 1.0
HE1 A:HIS64 3.5 27.7 1.0
C1 A:EDO304 3.6 50.9 1.0
H12 A:EDO304 3.7 61.1 1.0
H11 A:EDO304 3.8 61.1 1.0
HD21 A:ASN155 4.1 23.1 1.0
HE1 A:MET222 4.1 38.9 1.0
CG A:HIS64 4.3 23.1 1.0
ND1 A:HIS64 4.3 24.8 1.0
H A:CYS221 4.4 19.6 1.0
CA A:CYS221 4.4 15.4 1.0
O A:SER125 4.4 27.5 1.0
ND2 A:ASN155 4.7 19.2 1.0
HD22 A:ASN155 4.8 23.1 1.0
HB3 A:SER125 4.8 21.2 1.0
CE A:MET222 4.8 32.3 1.0
HE3 A:MET222 4.8 38.9 1.0
HA3 A:GLY219 4.8 28.0 1.0
HA A:CYS221 4.8 18.1 1.0
N A:CYS221 4.9 16.3 1.0
SD A:MET222 4.9 32.4 1.0
C2 A:EDO304 5.0 47.3 1.0

Calcium binding site 3 out of 4 in 6o44

Go back to Calcium Binding Sites List in 6o44
Calcium binding site 3 out of 4 in the Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca301

b:17.0
occ:1.00
 OE1 B:GLN2 2.2 23.0 1.0
O B:LEU75 2.4 19.3 1.0
OD1 B:ASN77 2.4 23.2 1.0
O B:VAL81 2.4 18.3 1.0
OD1 B:ASP41 2.4 21.2 1.0
O B:ILE79 2.5 18.5 1.0
OD2 B:ASP41 2.6 22.7 1.0
CG B:ASP41 2.8 36.6 1.0
CD B:GLN2 3.3 24.9 1.0
HG3 B:GLN2 3.3 31.8 1.0
CG B:ASN77 3.4 22.3 1.0
H B:ASN77 3.4 27.8 1.0
C B:LEU75 3.6 17.9 1.0
HG13 B:ILE79 3.6 33.5 1.0
C B:ILE79 3.6 26.4 1.0
C B:VAL81 3.6 17.4 1.0
H B:ILE79 3.6 23.9 1.0
H B:VAL81 3.6 26.7 1.0
HD21 B:ASN77 3.6 24.9 1.0
N B:VAL81 3.8 22.2 1.0
CG B:GLN2 3.8 26.4 1.0
ND2 B:ASN77 3.9 20.7 1.0
N B:ASN77 3.9 23.1 1.0
HA B:ASN76 4.0 28.4 1.0
HA3 B:GLY80 4.0 29.4 1.0
HB2 B:GLN2 4.1 26.7 1.0
C B:GLY80 4.1 25.4 1.0
HA B:LEU82 4.2 20.0 1.0
HB2 B:LEU75 4.2 27.1 1.0
HB1 B:ALA74 4.3 19.7 1.0
CA B:VAL81 4.4 17.6 1.0
N B:ILE79 4.4 20.2 1.0
HG12 B:ILE79 4.4 33.5 1.0
H B:LEU75 4.4 28.4 1.0
CB B:ASP41 4.4 21.1 1.0
N B:LEU75 4.4 23.6 1.0
CG1 B:ILE79 4.4 27.8 1.0
CA B:GLY80 4.4 24.4 1.0
NE2 B:GLN2 4.4 19.4 1.0
CA B:LEU75 4.4 20.8 1.0
N B:GLY80 4.5 23.1 1.0
N B:ASN76 4.5 22.8 1.0
CA B:ASN76 4.5 23.6 1.0
CB B:GLN2 4.5 22.2 1.0
CA B:ILE79 4.5 22.2 1.0
CB B:ASN77 4.6 27.7 1.0
HE21 B:GLN2 4.6 23.4 1.0
CA B:ASN77 4.6 19.7 1.0
C B:ASN76 4.6 28.4 1.0
HG2 B:GLN2 4.6 31.8 1.0
C B:ASN77 4.6 20.1 1.0
N B:LEU82 4.6 16.4 1.0
O B:ASN77 4.7 28.2 1.0
HA B:ASP41 4.7 24.9 1.0
HD22 B:ASN77 4.7 24.9 1.0
HB2 B:ASP41 4.7 25.0 1.0
O B:GLY80 4.8 19.7 1.0
HA B:VAL81 4.8 21.2 1.0
HB3 B:ASP41 4.8 25.0 1.0
CB B:LEU75 4.8 22.5 1.0
C B:ALA74 4.8 21.5 1.0
CA B:LEU82 4.8 16.6 1.0
HB2 B:LEU82 4.9 21.8 1.0

Calcium binding site 4 out of 4 in 6o44

Go back to Calcium Binding Sites List in 6o44
Calcium binding site 4 out of 4 in the Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca302

b:44.3
occ:1.00
 NE2 B:HIS64 2.3 22.8 1.0
SG B:CYS221 2.4 37.1 1.0
HO1 A:EDO307 2.5 0.1 1.0
HB2 B:CYS221 2.9 34.7 1.0
O1 A:EDO307 3.0 0.6 1.0
CD2 B:HIS64 3.0 24.0 1.0
CB B:CYS221 3.1 28.8 1.0
HB3 B:CYS221 3.1 34.7 1.0
HD2 B:HIS64 3.1 28.8 1.0
CE1 B:HIS64 3.4 26.3 1.0
HE1 B:HIS64 3.6 31.6 1.0
O B:HOH550 3.9 40.1 1.0
HE3 B:MET222 4.1 29.3 1.0
HD21 B:ASN155 4.2 26.9 1.0
CG B:HIS64 4.2 25.6 1.0
C1 A:EDO307 4.3 0.4 1.0
ND1 B:HIS64 4.4 26.1 1.0
H11 A:EDO307 4.4 0.5 1.0
O B:SER125 4.4 22.7 1.0
H B:CYS221 4.5 20.0 1.0
CA B:CYS221 4.5 16.6 1.0
H12 A:EDO307 4.7 0.5 1.0
CE B:MET222 4.8 24.3 1.0
HD22 B:ASN155 4.8 26.9 1.0
ND2 B:ASN155 4.8 22.4 1.0
HE2 B:MET222 4.8 29.3 1.0
HB3 B:SER125 4.9 24.6 1.0
HA3 B:GLY219 5.0 23.2 1.0
HA B:CYS221 5.0 19.5 1.0

Reference:

H.Tang, J.Zhang, K.Shi, H.Aihara, G.Du. Insight Into Subtilisin E-S7 Cleavage Pattern Based on Crystal Structure and Hydrolysates Peptide Analysis. Biochem. Biophys. Res. V. 512 623 2019COMMUN..
ISSN: ESSN 1090-2104
PubMed: 30914195
DOI: 10.1016/J.BBRC.2019.03.064
Page generated: Tue Jul 16 12:19:29 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy