Calcium in PDB 6oau: Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in Gnti-Deficient HEK293-F Cells

All present enzymatic activity of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in Gnti-Deficient HEK293-F Cells:
3.1.1.34;

The structure of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in Gnti-Deficient HEK293-F Cells, PDB code: 6oau was solved by R.Arora, P.A.Horton, T.E.Benson, M.J.Romanowski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.50 / 2.48
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	177.480, 97.250, 77.210, 90.00, 93.47, 90.00
R / Rfree (%)	21.3 / 24.6

The binding sites of Calcium atom in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in Gnti-Deficient HEK293-F Cells (pdb code 6oau). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in Gnti-Deficient HEK293-F Cells, PDB code: 6oau:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in Gnti-Deficient HEK293-F Cells


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in Gnti-Deficient HEK293-F Cells within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca504 b:78.7 occ:1.00 O A:ALA194 2.4 46.1 1.0 OD2 A:ASP202 2.5 64.3 1.0 O A:ARG197 2.6 44.2 1.0 OD1 A:ASP202 2.6 57.5 1.0 CG A:ASP202 2.9 55.8 1.0 O A:HOH629 3.2 64.6 1.0 C A:ALA194 3.5 47.2 1.0 OG A:SER199 3.6 51.7 1.0 CB A:SER199 3.6 43.6 1.0 C A:ARG197 3.8 43.6 1.0 N A:SER199 3.8 36.4 1.0 CA A:PRO195 4.0 42.0 1.0 O A:GLY166 4.1 41.0 1.0 N A:PRO195 4.2 42.7 1.0 C A:PRO195 4.2 44.2 1.0 C A:LEU198 4.3 35.9 1.0 O A:PRO195 4.3 45.4 1.0 CA A:SER199 4.3 37.9 1.0 CB A:ASP202 4.4 44.2 1.0 C A:GLY166 4.4 41.0 1.0 CA A:ALA194 4.4 44.6 1.0 N A:ARG197 4.5 39.4 1.0 CA A:LEU198 4.5 37.5 1.0 CB A:SER170 4.5 47.0 1.0 N A:LEU198 4.6 40.0 1.0 CB A:ALA194 4.7 44.9 1.0 CA A:ARG197 4.8 38.9 1.0 N A:ILE167 4.9 37.9 1.0 CA A:GLY166 4.9 37.1 1.0 N A:SER196 4.9 38.9 1.0 O A:LEU198 5.0 32.3 1.0

Calcium binding site 2 out of 2 in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in Gnti-Deficient HEK293-F Cells


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in Gnti-Deficient HEK293-F Cells within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca503 b:85.3 occ:1.00 OD1 B:ASP202 2.4 54.4 1.0 O B:ALA194 2.4 56.3 1.0 OD2 B:ASP202 2.6 62.7 1.0 O B:HOH628 2.6 52.3 1.0 CG B:ASP202 2.8 52.8 1.0 O B:ARG197 3.0 41.9 1.0 OG B:SER199 3.4 61.4 1.0 C B:ALA194 3.5 55.4 1.0 CB B:SER199 3.6 45.5 1.0 CA B:PRO195 4.0 48.5 1.0 N B:SER199 4.0 40.1 1.0 C B:ARG197 4.2 42.7 1.0 N B:PRO195 4.2 50.5 1.0 O B:GLY166 4.2 39.8 1.0 C B:PRO195 4.3 48.2 1.0 CB B:ASP202 4.3 41.4 1.0 CB B:SER170 4.4 39.5 1.0 O B:PRO195 4.4 48.2 1.0 CA B:SER199 4.4 41.9 1.0 CA B:ALA194 4.4 50.8 1.0 C B:LEU198 4.6 39.6 1.0 C B:GLY166 4.6 39.9 1.0 CB B:ALA194 4.6 51.4 1.0 N B:ARG197 4.8 43.0 1.0 CA B:LEU198 4.8 37.9 1.0 OG B:SER170 4.9 46.3 1.0 N B:LEU198 5.0 39.4 1.0

R.Arora, A.V.Nimonkar, D.Baird, C.Wang, C.H.Chiu, P.A.Horton, S.Hanrahan, R.Cubbon, S.Weldon, W.R.Tschantz, S.Mueller, R.Brunner, P.Lehr, P.Meier, J.Ottl, A.Voznesensky, P.Pandey, T.M.Smith, A.Stojanovic, A.Flyer, T.E.Benson, M.J.Romanowski, J.W.Trauger. Structure of Lipoprotein Lipase in Complex with GPIHBP1. Proc.Natl.Acad.Sci.Usa V. 116 10360 2019.
ISSN: ESSN 1091-6490
PubMed: 31072929
DOI: 10.1073/PNAS.1820171116