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Calcium in PDB 6oaz: Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells

Enzymatic activity of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells

All present enzymatic activity of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells:
3.1.1.34;

Protein crystallography data

The structure of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells, PDB code: 6oaz was solved by R.Arora, P.A.Horton, T.E.Benson, M.J.Romanowski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 121.52 / 3.04
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 157.000, 191.940, 96.060, 90.00, 90.00, 90.00
R / Rfree (%) 20.3 / 24.5

Calcium Binding Sites:

The binding sites of Calcium atom in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells (pdb code 6oaz). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 8 binding sites of Calcium where determined in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells, PDB code: 6oaz:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Calcium binding site 1 out of 8 in 6oaz

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Calcium binding site 1 out of 8 in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca505

b:80.9
occ:1.00
 OG A:SER199 2.2 94.8 1.0
OD1 A:ASP202 2.4 0.4 1.0
OD2 A:ASP202 2.5 0.6 1.0
O A:ALA194 2.5 98.5 1.0
O A:ARG197 2.7 88.3 1.0
CG A:ASP202 2.8 0.4 1.0
C A:ALA194 3.5 0.3 1.0
CB A:SER199 3.6 92.3 1.0
N A:SER199 3.9 92.0 1.0
CA A:PRO195 3.9 94.9 1.0
C A:ARG197 3.9 90.1 1.0
OD1 A:ASP201 4.0 0.7 1.0
OD2 A:ASP201 4.0 0.0 1.0
O A:PRO195 4.0 1.0 1.0
N A:PRO195 4.2 95.0 1.0
C A:PRO195 4.2 0.9 1.0
OG A:SER170 4.2 0.1 1.0
CA A:SER199 4.3 91.1 1.0
O A:GLY166 4.3 88.7 1.0
CB A:ASP202 4.3 94.8 1.0
CG A:ASP201 4.3 0.7 1.0
CB A:SER170 4.4 94.6 1.0
C A:GLY166 4.4 88.4 1.0
C A:LEU198 4.4 95.2 1.0
CA A:ALA194 4.6 0.5 1.0
CA A:LEU198 4.6 88.7 1.0
N A:ARG197 4.7 91.3 1.0
N A:LEU198 4.7 88.0 1.0
N A:ILE167 4.8 86.9 1.0
CA A:GLY166 4.9 81.5 1.0
CB A:ALA194 4.9 0.5 1.0
O A:SER199 4.9 90.4 1.0
CA A:ARG197 4.9 89.5 1.0
C A:SER199 5.0 94.3 1.0

Calcium binding site 2 out of 8 in 6oaz

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Calcium binding site 2 out of 8 in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca506

b:0.2
occ:1.00
 OE2 E:GLU122 2.8 0.4 1.0
OE1 A:GLU374 3.2 0.6 1.0
OE2 A:GLU374 3.3 0.3 1.0
CD A:GLU374 3.6 0.5 1.0
CD E:GLU122 3.9 0.9 1.0
OE1 E:GLU122 4.5 0.9 1.0
CG E:GLU122 5.0 0.5 1.0

Calcium binding site 3 out of 8 in 6oaz

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Calcium binding site 3 out of 8 in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca505

b:79.8
occ:1.00
 O B:ALA194 2.3 0.2 1.0
OG B:SER199 2.4 0.6 1.0
OD1 B:ASP202 2.6 99.4 1.0
O B:ARG197 2.6 89.3 1.0
OD2 B:ASP202 2.8 87.8 1.0
CG B:ASP202 3.0 96.0 1.0
C B:ALA194 3.3 0.9 1.0
CA B:PRO195 3.6 99.7 1.0
CB B:SER199 3.7 0.2 1.0
O B:PRO195 3.8 98.6 1.0
C B:ARG197 3.9 91.2 1.0
N B:PRO195 3.9 0.2 1.0
C B:PRO195 3.9 0.4 1.0
OD2 B:ASP201 4.0 0.8 1.0
OD1 B:ASP201 4.1 0.9 1.0
N B:SER199 4.1 93.1 1.0
OG B:SER170 4.2 0.1 1.0
CA B:ALA194 4.4 0.1 1.0
CB B:SER170 4.4 93.0 1.0
O B:GLY166 4.4 84.5 1.0
CG B:ASP201 4.4 0.5 1.0
CA B:SER199 4.5 94.5 1.0
N B:ARG197 4.5 90.6 1.0
C B:GLY166 4.5 87.7 1.0
CB B:ASP202 4.6 95.5 1.0
C B:LEU198 4.6 93.8 1.0
CA B:LEU198 4.7 86.6 1.0
N B:LEU198 4.7 87.4 1.0
CB B:ALA194 4.8 0.9 1.0
CA B:ARG197 4.8 89.8 1.0
N B:SER196 4.9 96.2 1.0
N B:ILE167 4.9 85.5 1.0
CB B:PRO195 5.0 0.1 1.0
CA B:GLY166 5.0 86.9 1.0

Calcium binding site 4 out of 8 in 6oaz

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Calcium binding site 4 out of 8 in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca506

b:0.2
occ:1.00
 OE1 B:GLU374 2.6 0.2 1.0
OE2 F:GLU122 2.7 0.2 1.0
OE1 F:GLU122 3.3 0.4 1.0
OE2 B:GLU374 3.3 0.2 1.0
CD B:GLU374 3.3 0.9 1.0
CD F:GLU122 3.4 0.0 1.0
CG B:GLU374 4.8 1.0 1.0
CG F:GLU122 4.8 0.2 1.0
OH B:TYR367 4.9 0.4 1.0

Calcium binding site 5 out of 8 in 6oaz

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Calcium binding site 5 out of 8 in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca505

b:63.8
occ:1.00
 OG C:SER199 2.2 0.0 1.0
OD1 C:ASP202 2.4 95.9 1.0
O C:ALA194 2.5 99.5 1.0
OD2 C:ASP202 2.7 0.3 1.0
O C:ARG197 2.8 90.5 1.0
CG C:ASP202 2.9 97.9 1.0
C C:ALA194 3.4 0.1 1.0
CB C:SER199 3.6 96.1 1.0
CA C:PRO195 3.8 90.7 1.0
OD1 C:ASP201 3.9 0.8 1.0
OD2 C:ASP201 3.9 0.3 1.0
N C:SER199 4.0 92.2 1.0
C C:ARG197 4.0 92.4 1.0
O C:PRO195 4.1 92.5 1.0
N C:PRO195 4.1 92.4 1.0
C C:PRO195 4.2 94.1 1.0
CG C:ASP201 4.2 0.2 1.0
OG C:SER170 4.3 0.2 1.0
CA C:SER199 4.3 92.5 1.0
CB C:SER170 4.4 97.7 1.0
CB C:ASP202 4.4 95.0 1.0
O C:GLY166 4.4 95.3 1.0
CA C:ALA194 4.5 0.5 1.0
C C:LEU198 4.5 94.3 1.0
C C:GLY166 4.6 94.7 1.0
N C:ARG197 4.7 90.3 1.0
CA C:LEU198 4.7 89.7 1.0
CB C:ALA194 4.8 0.6 1.0
N C:LEU198 4.8 90.0 1.0
O C:SER199 4.9 99.0 1.0
N C:ILE167 5.0 91.1 1.0
C C:SER199 5.0 98.7 1.0
CA C:ARG197 5.0 89.3 1.0

Calcium binding site 6 out of 8 in 6oaz

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Calcium binding site 6 out of 8 in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca506

b:0.0
occ:1.00
 OE2 G:GLU122 2.4 0.3 1.0
OE1 C:GLU374 2.7 0.2 1.0
OE2 C:GLU374 3.2 0.7 1.0
CD C:GLU374 3.3 0.8 1.0
CD G:GLU122 3.5 0.1 1.0
OE1 G:GLU122 3.8 0.8 1.0
CG G:GLU122 4.8 0.6 1.0
CG C:GLU374 4.8 0.3 1.0
OH C:TYR367 5.0 0.1 1.0

Calcium binding site 7 out of 8 in 6oaz

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Calcium binding site 7 out of 8 in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Ca505

b:71.0
occ:1.00
 OG D:SER199 2.5 96.4 1.0
O D:ALA194 2.5 0.0 1.0
OD1 D:ASP202 2.5 99.2 1.0
OD2 D:ASP202 2.7 94.8 1.0
O D:ARG197 2.7 85.2 1.0
CG D:ASP202 3.0 97.3 1.0
C D:ALA194 3.4 0.0 1.0
CA D:PRO195 3.6 95.8 1.0
O D:PRO195 3.8 0.2 1.0
CB D:SER199 3.8 89.6 1.0
C D:PRO195 3.9 0.8 1.0
N D:PRO195 3.9 96.3 1.0
C D:ARG197 4.0 85.6 1.0
OD2 D:ASP201 4.0 0.7 1.0
OG D:SER170 4.1 0.9 1.0
OD1 D:ASP201 4.1 0.4 1.0
N D:SER199 4.1 84.5 1.0
CB D:SER170 4.3 94.3 1.0
O D:GLY166 4.3 90.9 1.0
CG D:ASP201 4.4 0.6 1.0
C D:GLY166 4.5 90.4 1.0
CB D:ASP202 4.5 92.2 1.0
CA D:ALA194 4.5 0.6 1.0
N D:ARG197 4.5 88.8 1.0
CA D:SER199 4.6 85.7 1.0
C D:LEU198 4.7 85.3 1.0
CA D:LEU198 4.8 78.4 1.0
N D:ILE167 4.8 87.8 1.0
N D:SER196 4.8 97.2 1.0
N D:LEU198 4.8 78.8 1.0
CB D:ALA194 4.9 0.5 1.0
CA D:ARG197 4.9 87.4 1.0
CB D:PRO195 4.9 96.1 1.0
CA D:GLY166 5.0 85.7 1.0

Calcium binding site 8 out of 8 in 6oaz

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Calcium binding site 8 out of 8 in the Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 8 of Apo Structure of Wt Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D Produced in HEK293-F Cells within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Ca506

b:0.7
occ:1.00
 OE1 D:GLU374 2.7 0.3 1.0
OE2 H:GLU122 2.7 0.1 1.0
OE2 D:GLU374 2.9 0.4 1.0
CD D:GLU374 3.2 0.9 1.0
CD H:GLU122 3.6 0.8 1.0
OE1 H:GLU122 4.0 0.8 1.0
CG D:GLU374 4.7 0.0 1.0
CG H:GLU122 4.9 0.0 1.0

Reference:

R.Arora, A.V.Nimonkar, D.Baird, C.Wang, C.H.Chiu, P.A.Horton, S.Hanrahan, R.Cubbon, S.Weldon, W.R.Tschantz, S.Mueller, R.Brunner, P.Lehr, P.Meier, J.Ottl, A.Voznesensky, P.Pandey, T.M.Smith, A.Stojanovic, A.Flyer, T.E.Benson, M.J.Romanowski, J.W.Trauger. Structure of Lipoprotein Lipase in Complex with GPIHBP1. Proc.Natl.Acad.Sci.Usa V. 116 10360 2019.
ISSN: ESSN 1091-6490
PubMed: 31072929
DOI: 10.1073/PNAS.1820171116
Page generated: Tue Jul 16 12:23:29 2024

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