Calcium in PDB 6pol: Crystal Structure of the Human NELL1 EGF1-3-ROBO3 FN1 Complex
Protein crystallography data
The structure of Crystal Structure of the Human NELL1 EGF1-3-ROBO3 FN1 Complex, PDB code: 6pol
was solved by
J.Wang,
J.S.Pak,
E.Ozkan,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
51.47 /
1.80
|
Space group
|
P 31 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
87.000,
87.000,
211.300,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
23 /
26.7
|
Calcium Binding Sites:
The binding sites of Calcium atom in the Crystal Structure of the Human NELL1 EGF1-3-ROBO3 FN1 Complex
(pdb code 6pol). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the
Crystal Structure of the Human NELL1 EGF1-3-ROBO3 FN1 Complex, PDB code: 6pol:
Jump to Calcium binding site number:
1;
2;
3;
Calcium binding site 1 out
of 3 in 6pol
Go back to
Calcium Binding Sites List in 6pol
Calcium binding site 1 out
of 3 in the Crystal Structure of the Human NELL1 EGF1-3-ROBO3 FN1 Complex
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Crystal Structure of the Human NELL1 EGF1-3-ROBO3 FN1 Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca902
b:29.9
occ:1.00
|
O
|
B:ILE435
|
2.3
|
34.7
|
1.0
|
O
|
B:HOH1006
|
2.4
|
31.8
|
1.0
|
O
|
B:LEU454
|
2.4
|
30.1
|
1.0
|
OD1
|
B:ASN453
|
2.4
|
31.6
|
1.0
|
O
|
B:LEU457
|
2.5
|
28.8
|
1.0
|
OD1
|
B:ASP434
|
2.5
|
32.0
|
1.0
|
OE1
|
B:GLU437
|
2.6
|
37.0
|
1.0
|
OD2
|
B:ASP434
|
2.6
|
33.6
|
1.0
|
CG
|
B:ASP434
|
2.9
|
34.2
|
1.0
|
CG
|
B:ASN453
|
3.5
|
34.2
|
1.0
|
C
|
B:ILE435
|
3.5
|
33.3
|
1.0
|
CD
|
B:GLU437
|
3.5
|
35.8
|
1.0
|
C
|
B:LEU454
|
3.6
|
33.6
|
1.0
|
C
|
B:LEU457
|
3.6
|
31.1
|
1.0
|
N
|
B:LEU457
|
3.7
|
32.3
|
1.0
|
OE2
|
B:GLU437
|
3.8
|
35.4
|
1.0
|
N
|
B:LEU454
|
3.8
|
33.5
|
1.0
|
ND2
|
B:ASN453
|
3.9
|
32.8
|
1.0
|
N
|
B:GLY456
|
4.0
|
34.7
|
1.0
|
CA
|
B:LEU457
|
4.3
|
36.4
|
1.0
|
N
|
B:ILE435
|
4.3
|
33.2
|
1.0
|
CA
|
B:LEU454
|
4.3
|
34.7
|
1.0
|
C
|
B:GLY456
|
4.4
|
34.9
|
1.0
|
CA
|
B:ILE435
|
4.4
|
32.9
|
1.0
|
N
|
B:ASP436
|
4.4
|
35.3
|
1.0
|
CB
|
B:ASP434
|
4.4
|
31.8
|
1.0
|
CA
|
B:ASP436
|
4.5
|
37.8
|
1.0
|
N
|
B:GLU437
|
4.5
|
33.9
|
1.0
|
CA
|
B:GLY456
|
4.6
|
34.8
|
1.0
|
N
|
B:PRO455
|
4.6
|
36.6
|
1.0
|
O
|
B:GLY421
|
4.7
|
32.0
|
1.0
|
N
|
B:TYR458
|
4.7
|
31.4
|
1.0
|
CA
|
B:PRO455
|
4.7
|
39.1
|
1.0
|
CB
|
B:ILE435
|
4.7
|
33.6
|
1.0
|
C
|
B:PRO455
|
4.7
|
38.5
|
1.0
|
CB
|
B:ASN453
|
4.8
|
36.5
|
1.0
|
C
|
B:ASP434
|
4.8
|
33.8
|
1.0
|
O
|
B:HOH1027
|
4.8
|
45.0
|
1.0
|
C
|
B:ASN453
|
4.8
|
35.2
|
1.0
|
CB
|
B:TYR458
|
4.9
|
27.1
|
1.0
|
CA
|
B:ASN453
|
4.9
|
34.4
|
1.0
|
CG
|
B:GLU437
|
4.9
|
33.9
|
1.0
|
|
Calcium binding site 2 out
of 3 in 6pol
Go back to
Calcium Binding Sites List in 6pol
Calcium binding site 2 out
of 3 in the Crystal Structure of the Human NELL1 EGF1-3-ROBO3 FN1 Complex
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Crystal Structure of the Human NELL1 EGF1-3-ROBO3 FN1 Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Ca902
b:30.8
occ:1.00
|
O
|
D:ILE435
|
2.3
|
33.1
|
1.0
|
OD1
|
D:ASN453
|
2.4
|
30.2
|
1.0
|
O
|
D:HOH1020
|
2.4
|
23.8
|
1.0
|
O
|
D:LEU454
|
2.5
|
30.2
|
1.0
|
OE1
|
D:GLU437
|
2.5
|
35.1
|
1.0
|
O
|
D:LEU457
|
2.5
|
30.6
|
1.0
|
OD1
|
D:ASP434
|
2.5
|
33.9
|
1.0
|
OD2
|
D:ASP434
|
2.6
|
35.8
|
1.0
|
CG
|
D:ASP434
|
2.9
|
36.3
|
1.0
|
CG
|
D:ASN453
|
3.4
|
32.6
|
1.0
|
C
|
D:ILE435
|
3.5
|
33.9
|
1.0
|
CD
|
D:GLU437
|
3.5
|
33.4
|
1.0
|
C
|
D:LEU457
|
3.7
|
34.0
|
1.0
|
C
|
D:LEU454
|
3.7
|
34.7
|
1.0
|
OE2
|
D:GLU437
|
3.8
|
34.1
|
1.0
|
N
|
D:LEU457
|
3.8
|
32.1
|
1.0
|
ND2
|
D:ASN453
|
3.8
|
32.3
|
1.0
|
N
|
D:LEU454
|
3.9
|
32.9
|
1.0
|
N
|
D:GLY456
|
4.1
|
35.5
|
1.0
|
CA
|
D:LEU457
|
4.3
|
35.2
|
1.0
|
N
|
D:ILE435
|
4.3
|
32.5
|
1.0
|
CB
|
D:ASP434
|
4.4
|
35.1
|
1.0
|
CA
|
D:ILE435
|
4.4
|
33.7
|
1.0
|
CA
|
D:LEU454
|
4.4
|
33.1
|
1.0
|
C
|
D:GLY456
|
4.4
|
34.7
|
1.0
|
N
|
D:ASP436
|
4.4
|
36.3
|
1.0
|
N
|
D:GLU437
|
4.5
|
34.7
|
1.0
|
CA
|
D:ASP436
|
4.5
|
35.4
|
1.0
|
O
|
D:GLY421
|
4.6
|
31.4
|
1.0
|
CA
|
D:GLY456
|
4.7
|
32.3
|
1.0
|
CB
|
D:ASN453
|
4.7
|
33.1
|
1.0
|
N
|
D:PRO455
|
4.7
|
36.5
|
1.0
|
N
|
D:TYR458
|
4.7
|
29.4
|
1.0
|
CB
|
D:ILE435
|
4.8
|
32.5
|
1.0
|
C
|
D:ASP434
|
4.8
|
32.5
|
1.0
|
C
|
D:ASN453
|
4.8
|
33.8
|
1.0
|
CA
|
D:PRO455
|
4.8
|
38.8
|
1.0
|
C
|
D:PRO455
|
4.8
|
37.1
|
1.0
|
CA
|
D:ASN453
|
4.8
|
31.8
|
1.0
|
CB
|
D:TYR458
|
4.9
|
30.5
|
1.0
|
CG
|
D:GLU437
|
4.9
|
32.1
|
1.0
|
O
|
D:HOH1078
|
5.0
|
49.4
|
1.0
|
|
Calcium binding site 3 out
of 3 in 6pol
Go back to
Calcium Binding Sites List in 6pol
Calcium binding site 3 out
of 3 in the Crystal Structure of the Human NELL1 EGF1-3-ROBO3 FN1 Complex
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Crystal Structure of the Human NELL1 EGF1-3-ROBO3 FN1 Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Ca902
b:29.8
occ:1.00
|
O
|
F:ILE435
|
2.3
|
34.8
|
1.0
|
OD1
|
F:ASN453
|
2.4
|
31.9
|
1.0
|
O
|
F:LEU454
|
2.4
|
34.3
|
1.0
|
O
|
F:LEU457
|
2.5
|
34.8
|
1.0
|
O
|
F:HOH1023
|
2.5
|
29.7
|
1.0
|
OD1
|
F:ASP434
|
2.5
|
34.3
|
1.0
|
OD2
|
F:ASP434
|
2.6
|
35.6
|
1.0
|
OE1
|
F:GLU437
|
2.6
|
32.8
|
1.0
|
CG
|
F:ASP434
|
2.9
|
37.0
|
1.0
|
CG
|
F:ASN453
|
3.4
|
37.4
|
1.0
|
C
|
F:ILE435
|
3.5
|
34.2
|
1.0
|
C
|
F:LEU457
|
3.6
|
31.5
|
1.0
|
C
|
F:LEU454
|
3.6
|
36.3
|
1.0
|
CD
|
F:GLU437
|
3.6
|
34.6
|
1.0
|
N
|
F:LEU457
|
3.8
|
36.6
|
1.0
|
N
|
F:LEU454
|
3.8
|
37.2
|
1.0
|
ND2
|
F:ASN453
|
3.9
|
40.3
|
1.0
|
OE2
|
F:GLU437
|
3.9
|
33.8
|
1.0
|
N
|
F:GLY456
|
4.1
|
36.7
|
1.0
|
CA
|
F:LEU457
|
4.2
|
36.3
|
1.0
|
N
|
F:ILE435
|
4.3
|
32.8
|
1.0
|
CA
|
F:LEU454
|
4.3
|
35.8
|
1.0
|
CB
|
F:ASP434
|
4.4
|
37.1
|
1.0
|
CA
|
F:ILE435
|
4.4
|
35.3
|
1.0
|
N
|
F:ASP436
|
4.4
|
33.7
|
1.0
|
N
|
F:GLU437
|
4.5
|
34.4
|
1.0
|
CA
|
F:ASP436
|
4.5
|
36.1
|
1.0
|
C
|
F:GLY456
|
4.5
|
36.8
|
1.0
|
N
|
F:PRO455
|
4.6
|
38.4
|
1.0
|
N
|
F:TYR458
|
4.7
|
31.4
|
1.0
|
CB
|
F:ASN453
|
4.7
|
38.1
|
1.0
|
CA
|
F:PRO455
|
4.7
|
37.5
|
1.0
|
CA
|
F:GLY456
|
4.7
|
37.3
|
1.0
|
O
|
F:GLY421
|
4.7
|
34.8
|
1.0
|
C
|
F:ASN453
|
4.7
|
37.3
|
1.0
|
C
|
F:ASP434
|
4.7
|
35.9
|
1.0
|
CB
|
F:ILE435
|
4.8
|
35.7
|
1.0
|
C
|
F:PRO455
|
4.8
|
38.3
|
1.0
|
CA
|
F:ASN453
|
4.8
|
35.9
|
1.0
|
CB
|
F:TYR458
|
4.9
|
33.8
|
1.0
|
CG
|
F:GLU437
|
5.0
|
32.1
|
1.0
|
O
|
F:HOH1088
|
5.0
|
45.9
|
1.0
|
|
Reference:
J.S.Pak,
Z.J.Deloughery,
J.Wang,
N.Acharya,
Y.Park,
A.Jaworski,
E.Ozkan.
NELL2-ROBO3 Complex Structure Reveals Mechanisms of Receptor Activation For Axon Guidance. Nat Commun V. 11 1489 2020.
ISSN: ESSN 2041-1723
PubMed: 32198364
DOI: 10.1038/S41467-020-15211-1
Page generated: Tue Jul 16 12:56:50 2024
|