Calcium in PDB 6rb2: Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form

All present enzymatic activity of Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form:
7.2.2.10;

The structure of Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form, PDB code: 6rb2 was solved by J.D.Clausen, C.Montigny, G.Lenoir, B.Arnou, C.Jaxel, J.V.Moller, P.Nissen, J.P.Andersen, M.Le Maire, M.Bublitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.81 / 3.20
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	232.900, 126.990, 49.810, 90.00, 90.00, 90.00
R / Rfree (%)	21.2 / 25.7

The structure of Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form also contains other interesting chemical elements:

Potassium

(K)

1 atom

The binding sites of Calcium atom in the Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form (pdb code 6rb2). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form, PDB code: 6rb2:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1002 b:77.2 occ:1.00 OD1 A:ASP800 2.3 94.7 1.0 OE2 A:GLU771 2.3 85.2 1.0 OE2 A:GLU908 2.4 0.3 1.0 OD1 A:ASN768 2.4 81.0 1.0 OG1 A:THR799 2.4 75.9 1.0 CD A:GLU771 3.2 88.2 1.0 CD A:GLU908 3.2 85.6 1.0 OE1 A:GLU908 3.3 77.1 1.0 CG A:ASP800 3.4 93.9 1.0 CB A:THR799 3.4 73.8 1.0 CG A:ASN768 3.5 73.5 1.0 OE1 A:GLU771 3.7 96.4 1.0 OD2 A:ASP800 3.9 99.0 1.0 ND2 A:ASN768 4.1 74.0 1.0 CB A:SER767 4.2 98.8 1.0 C A:THR799 4.2 71.5 1.0 CG A:GLU771 4.2 83.1 1.0 N A:ASP800 4.3 81.4 1.0 N A:ASN768 4.4 76.7 1.0 CG2 A:THR799 4.5 70.7 1.0 CA A:THR799 4.5 78.8 1.0 O A:THR799 4.5 63.0 1.0 CA A:ASN768 4.5 76.3 1.0 CG A:GLU908 4.6 83.6 1.0 C A:SER767 4.6 85.8 1.0 CB A:ASN768 4.6 70.0 1.0 CB A:ASP800 4.7 87.8 1.0 O A:VAL795 4.7 0.2 1.0 CA A:ASP800 4.8 84.2 1.0 O A:SER767 4.8 88.9 1.0

Calcium binding site 2 out of 3 in the Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1003 b:0.6 occ:1.00 O A:ILE307 2.2 79.6 1.0 OD1 A:ASN796 2.4 98.9 1.0 OD2 A:ASP800 2.4 99.0 1.0 OE1 A:GLU309 2.4 0.5 1.0 OE2 A:GLU309 2.4 0.9 1.0 O A:ALA305 2.7 79.0 1.0 O A:VAL304 2.7 90.9 1.0 CD A:GLU309 2.7 1.0 1.0 CG A:ASP800 3.2 93.9 1.0 C A:ILE307 3.3 73.2 1.0 CB A:ASP800 3.3 87.8 1.0 C A:ALA305 3.5 75.0 1.0 CG A:ASN796 3.5 95.9 1.0 C A:VAL304 3.9 82.2 1.0 N A:ILE307 4.0 77.6 1.0 CA A:ALA305 4.0 69.6 1.0 N A:GLU309 4.2 80.6 1.0 ND2 A:ASN768 4.2 74.0 1.0 CA A:ILE307 4.2 72.4 1.0 ND2 A:ASN796 4.2 95.0 1.0 CG A:GLU309 4.2 0.9 1.0 N A:PRO308 4.2 67.8 1.0 OD1 A:ASP800 4.4 94.7 1.0 C A:ALA306 4.4 77.8 1.0 CA A:PRO308 4.4 72.2 1.0 N A:ALA305 4.4 70.8 1.0 N A:ALA306 4.5 78.8 1.0 CB A:ASN796 4.7 94.0 1.0 O A:ALA306 4.8 70.6 1.0 CB A:ILE307 4.8 69.6 1.0 CA A:ASP800 4.8 84.2 1.0 CB A:GLU309 4.9 94.7 1.0 C A:PRO308 4.9 78.7 1.0 CA A:ALA306 4.9 83.5 1.0 O A:ASN796 5.0 83.8 1.0

Calcium binding site 3 out of 3 in the Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Caamppcp Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1005 b:40.7 occ:1.00 OD1 A:ASP703 2.3 47.4 1.0 O A:THR353 2.3 38.6 1.0 OD2 A:ASP351 2.4 48.9 1.0 O A:HOH1101 2.4 56.4 1.0 O A:HOH1102 2.4 20.3 1.0 O3G A:ACP1004 2.5 57.4 1.0 CG A:ASP703 3.0 51.0 1.0 OD2 A:ASP703 3.0 68.9 1.0 C A:THR353 3.5 41.6 1.0 CG A:ASP351 3.5 60.8 1.0 PG A:ACP1004 3.9 37.3 1.0 O A:HOH1104 4.0 65.5 1.0 OD1 A:ASP351 4.1 77.7 1.0 CB A:THR353 4.1 47.6 1.0 C3B A:ACP1004 4.2 83.8 1.0 CA A:THR353 4.3 46.8 1.0 N A:GLY704 4.3 34.4 1.0 OD2 A:ASP707 4.4 56.1 1.0 CB A:ASP703 4.5 42.3 1.0 N A:GLY354 4.6 39.7 1.0 CG2 A:THR353 4.6 49.8 1.0 N A:THR353 4.6 43.1 1.0 CB A:ASP351 4.7 52.9 1.0 CA A:GLY704 4.7 47.2 1.0 CA A:GLY354 4.8 47.2 1.0 O2G A:ACP1004 4.8 28.7 1.0 O1G A:ACP1004 4.9 35.5 1.0 C A:ASP703 4.9 48.5 1.0 N A:ASP703 5.0 34.9 1.0

J.D.Clausen, C.Montigny, G.Lenoir, B.Arnou, C.Jaxel, M.Le Maire, J.P.Andersen, J.V.Moller, P.Nissen, M.Bublitz. Structure of the (Sr)CA2+-Atpase Mutant E340A in the CA2-E1-Mgamppcp Form To Be Published.