Atomistry » Calcium » PDB 6suw-6t6v » 6t18
Atomistry »
  Calcium »
    PDB 6suw-6t6v »
      6t18 »

Calcium in PDB 6t18: Asr Alternansucrase in Complex with Oligoalternan

Enzymatic activity of Asr Alternansucrase in Complex with Oligoalternan

All present enzymatic activity of Asr Alternansucrase in Complex with Oligoalternan:
2.4.1.140;

Protein crystallography data

The structure of Asr Alternansucrase in Complex with Oligoalternan, PDB code: 6t18 was solved by G.Cioci, M.Molina, C.Moulis, M.Remaud-Simeon, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 3.15
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 100.876, 135.654, 239.424, 90.00, 90.00, 90.00
R / Rfree (%) 20 / 22.6

Calcium Binding Sites:

The binding sites of Calcium atom in the Asr Alternansucrase in Complex with Oligoalternan (pdb code 6t18). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Asr Alternansucrase in Complex with Oligoalternan, PDB code: 6t18:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 6t18

Go back to Calcium Binding Sites List in 6t18
Calcium binding site 1 out of 2 in the Asr Alternansucrase in Complex with Oligoalternan


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Asr Alternansucrase in Complex with Oligoalternan within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca1501

b:69.9
occ:1.00
OD1 A:ASP595 1.6 66.6 1.0
OE2 A:GLU589 1.9 62.4 1.0
OD1 A:ASP1173 2.1 59.8 1.0
O A:GLU589 2.2 52.2 1.0
O A:ASN639 2.3 57.5 1.0
CG A:ASP595 2.5 63.9 1.0
OD2 A:ASP595 2.8 74.3 1.0
CD A:GLU589 3.0 61.6 1.0
CG A:ASP1173 3.3 58.2 1.0
C A:GLU589 3.3 52.1 1.0
CG A:GLU589 3.5 58.7 1.0
C A:ASN639 3.6 54.6 1.0
OD2 A:ASP1173 3.9 59.2 1.0
CA A:GLU589 3.9 54.5 1.0
CB A:ASP595 4.0 59.3 1.0
OE1 A:GLU589 4.1 63.5 1.0
CB A:GLU589 4.3 57.0 1.0
O A:ASP1173 4.3 61.8 1.0
CA A:VAL640 4.3 52.8 1.0
N A:VAL640 4.4 52.5 1.0
CG1 A:VAL640 4.4 52.8 1.0
N A:PHE590 4.4 50.4 1.0
O A:ILE596 4.5 51.4 1.0
CB A:ASP1173 4.5 57.6 1.0
CB A:ASN639 4.5 57.0 1.0
CA A:ASN639 4.6 53.6 1.0
ND2 A:ASN1263 4.7 58.9 1.0
CA A:ASP1173 4.8 56.6 1.0
CA A:PHE590 4.8 52.8 1.0
CA A:ASP595 4.8 56.8 1.0
C A:ASP1173 4.9 56.1 1.0
CB A:VAL640 4.9 52.0 1.0

Calcium binding site 2 out of 2 in 6t18

Go back to Calcium Binding Sites List in 6t18
Calcium binding site 2 out of 2 in the Asr Alternansucrase in Complex with Oligoalternan


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Asr Alternansucrase in Complex with Oligoalternan within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca1501

b:70.5
occ:1.00
OD1 B:ASP595 1.4 77.3 1.0
OD1 B:ASP1173 2.0 70.4 1.0
OE2 B:GLU589 2.1 71.0 1.0
O B:GLU589 2.2 63.7 1.0
CG B:ASP595 2.4 70.1 1.0
O B:ASN639 2.5 58.7 1.0
OD2 B:ASP595 2.8 77.1 1.0
CG B:ASP1173 3.1 68.6 1.0
CD B:GLU589 3.2 73.7 1.0
C B:GLU589 3.4 62.7 1.0
CG B:GLU589 3.6 71.5 1.0
OD2 B:ASP1173 3.7 65.1 1.0
C B:ASN639 3.7 59.5 1.0
CB B:ASP595 3.8 64.5 1.0
CA B:GLU589 4.0 67.2 1.0
O B:ASP1173 4.1 62.4 1.0
OE1 B:GLU589 4.3 77.8 1.0
CB B:ASP1173 4.3 67.7 1.0
O B:ILE596 4.4 60.4 1.0
CB B:GLU589 4.4 70.4 1.0
CG1 B:VAL640 4.4 62.4 1.0
N B:PHE590 4.5 58.9 1.0
CA B:VAL640 4.5 63.0 1.0
N B:VAL640 4.6 61.3 1.0
CB B:ASN639 4.6 60.2 1.0
CA B:ASP1173 4.6 65.5 1.0
CA B:ASP595 4.6 62.7 1.0
CA B:ASN639 4.7 60.9 1.0
C B:ASP1173 4.7 63.6 1.0
ND2 B:ASN1263 4.8 65.2 1.0
CA B:PHE590 4.8 59.5 1.0

Reference:

M.Molina, C.Moulis, N.Monties, D.Guieysse, S.Morel, G.Cioci, M.Remaud-Simeon. A Specific Oligosaccharide-Binding Site in Alternansucrase Catalytic Domain Mediates Alternan Elongation. J.Biol.Chem. 2020.
ISSN: ESSN 1083-351X
PubMed: 32409580
DOI: 10.1074/JBC.RA120.013028
Page generated: Tue Jul 16 15:04:56 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy