Calcium in PDB 6t8f: Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose
Enzymatic activity of Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose
All present enzymatic activity of Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose:
5.3.1.5;
Protein crystallography data
The structure of Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose, PDB code: 6t8f
was solved by
H.J.Rozeboom,
D.B.Janssen,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
46.60 /
2.00
|
Space group
|
P 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
78.598,
79.372,
91.982,
115.46,
89.98,
117.13
|
R / Rfree (%)
|
14.9 /
18.5
|
Calcium Binding Sites:
The binding sites of Calcium atom in the Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose
(pdb code 6t8f). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 8 binding sites of Calcium where determined in the
Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose, PDB code: 6t8f:
Jump to Calcium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Calcium binding site 1 out
of 8 in 6t8f
Go back to
Calcium Binding Sites List in 6t8f
Calcium binding site 1 out
of 8 in the Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca501
b:16.5
occ:1.00
|
OD2
|
A:ASP297
|
2.1
|
19.7
|
1.0
|
OE2
|
A:GLU233
|
2.1
|
19.9
|
1.0
|
OE1
|
A:GLU269
|
2.1
|
21.7
|
1.0
|
O2
|
A:XLS503
|
2.2
|
29.7
|
1.0
|
OD2
|
A:ASP340
|
2.2
|
20.9
|
1.0
|
O4
|
A:XLS503
|
2.3
|
29.4
|
1.0
|
CD
|
A:GLU233
|
2.9
|
18.5
|
1.0
|
OE1
|
A:GLU233
|
3.1
|
21.1
|
1.0
|
C2
|
A:XLS503
|
3.2
|
33.8
|
1.0
|
CG
|
A:ASP297
|
3.2
|
19.9
|
1.0
|
C4
|
A:XLS503
|
3.3
|
32.5
|
1.0
|
CG
|
A:ASP340
|
3.3
|
19.1
|
1.0
|
CD
|
A:GLU269
|
3.3
|
19.3
|
1.0
|
C3
|
A:XLS503
|
3.5
|
34.6
|
1.0
|
O3
|
A:XLS503
|
3.6
|
36.9
|
1.0
|
CB
|
A:ASP297
|
3.8
|
17.9
|
1.0
|
CB
|
A:ASP340
|
3.8
|
17.4
|
1.0
|
O
|
A:HOH614
|
3.9
|
20.3
|
1.0
|
CE1
|
A:HIS272
|
3.9
|
17.5
|
1.0
|
CG
|
A:GLU269
|
4.1
|
18.6
|
1.0
|
CB
|
A:GLU269
|
4.1
|
17.8
|
1.0
|
OE2
|
A:GLU269
|
4.3
|
20.2
|
1.0
|
CG
|
A:GLU233
|
4.3
|
18.4
|
1.0
|
OD1
|
A:ASP297
|
4.3
|
20.1
|
1.0
|
OD1
|
A:ASP340
|
4.4
|
19.7
|
1.0
|
NE2
|
A:HIS272
|
4.5
|
17.9
|
1.0
|
ND1
|
A:HIS272
|
4.6
|
16.1
|
1.0
|
C1
|
A:XLS503
|
4.6
|
32.9
|
1.0
|
ND2
|
A:ASN267
|
4.6
|
16.4
|
1.0
|
C5
|
A:XLS503
|
4.7
|
32.2
|
1.0
|
CA
|
A:CA502
|
4.8
|
21.0
|
1.0
|
|
Calcium binding site 2 out
of 8 in 6t8f
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Calcium Binding Sites List in 6t8f
Calcium binding site 2 out
of 8 in the Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca502
b:21.0
occ:1.00
|
OD1
|
A:ASP310
|
1.9
|
22.0
|
1.0
|
OD2
|
A:ASP308
|
1.9
|
33.0
|
1.0
|
O
|
A:HOH614
|
2.0
|
20.3
|
1.0
|
OE2
|
A:GLU269
|
2.0
|
20.2
|
1.0
|
CG
|
A:ASP308
|
2.6
|
29.8
|
1.0
|
OD1
|
A:ASP308
|
2.7
|
35.7
|
1.0
|
CG
|
A:ASP310
|
2.9
|
21.0
|
1.0
|
CD
|
A:GLU269
|
2.9
|
19.3
|
1.0
|
NE2
|
A:HIS272
|
3.1
|
17.9
|
1.0
|
OD2
|
A:ASP310
|
3.1
|
21.4
|
1.0
|
OE1
|
A:GLU269
|
3.2
|
21.7
|
1.0
|
O
|
A:HOH675
|
3.5
|
25.4
|
1.0
|
CD2
|
A:HIS272
|
3.6
|
16.5
|
1.0
|
O2
|
A:XLS503
|
3.7
|
29.7
|
1.0
|
ND2
|
A:ASN299
|
3.8
|
13.6
|
1.0
|
O
|
A:HOH898
|
4.0
|
37.7
|
1.0
|
CB
|
A:ASP308
|
4.1
|
25.6
|
1.0
|
CE1
|
A:HIS272
|
4.1
|
17.5
|
1.0
|
CG
|
A:GLU269
|
4.3
|
18.6
|
1.0
|
OD2
|
A:ASP340
|
4.3
|
20.9
|
1.0
|
CB
|
A:ASP310
|
4.3
|
20.0
|
1.0
|
CG
|
A:ASP340
|
4.5
|
19.1
|
1.0
|
OD1
|
A:ASP340
|
4.6
|
19.7
|
1.0
|
CB
|
A:ASN271
|
4.6
|
17.2
|
1.0
|
O1
|
A:XLS503
|
4.7
|
33.8
|
1.0
|
O
|
A:ASP340
|
4.7
|
15.6
|
1.0
|
C2
|
A:XLS503
|
4.8
|
33.8
|
1.0
|
CA
|
A:ASP310
|
4.8
|
18.6
|
1.0
|
CA
|
A:CA501
|
4.8
|
16.5
|
1.0
|
CG
|
A:HIS272
|
4.8
|
16.7
|
1.0
|
C1
|
A:XLS503
|
4.9
|
32.9
|
1.0
|
N
|
A:ASP310
|
5.0
|
17.3
|
1.0
|
|
Calcium binding site 3 out
of 8 in 6t8f
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Calcium Binding Sites List in 6t8f
Calcium binding site 3 out
of 8 in the Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca501
b:16.5
occ:1.00
|
OE2
|
B:GLU233
|
2.1
|
19.4
|
1.0
|
OD2
|
B:ASP297
|
2.1
|
22.1
|
1.0
|
O2
|
B:XLS503
|
2.1
|
34.5
|
1.0
|
OE1
|
B:GLU269
|
2.1
|
23.9
|
1.0
|
OD2
|
B:ASP340
|
2.2
|
19.9
|
1.0
|
O4
|
B:XLS503
|
2.4
|
32.3
|
1.0
|
CD
|
B:GLU233
|
2.9
|
18.4
|
1.0
|
OE1
|
B:GLU233
|
3.1
|
18.3
|
1.0
|
CG
|
B:ASP297
|
3.2
|
19.1
|
1.0
|
C2
|
B:XLS503
|
3.3
|
38.2
|
1.0
|
CG
|
B:ASP340
|
3.3
|
19.6
|
1.0
|
CD
|
B:GLU269
|
3.3
|
22.4
|
1.0
|
C4
|
B:XLS503
|
3.4
|
35.3
|
1.0
|
CB
|
B:ASP297
|
3.7
|
17.6
|
1.0
|
O
|
B:HOH637
|
3.7
|
26.3
|
1.0
|
CB
|
B:ASP340
|
3.8
|
18.0
|
1.0
|
C3
|
B:XLS503
|
3.8
|
38.0
|
1.0
|
CE1
|
B:HIS272
|
4.0
|
22.3
|
1.0
|
CG
|
B:GLU269
|
4.1
|
20.6
|
1.0
|
CB
|
B:GLU269
|
4.1
|
18.7
|
1.0
|
OD1
|
B:ASP297
|
4.3
|
19.0
|
1.0
|
OE2
|
B:GLU269
|
4.3
|
23.6
|
1.0
|
CG
|
B:GLU233
|
4.3
|
18.7
|
1.0
|
OD1
|
B:ASP340
|
4.4
|
21.8
|
1.0
|
NE2
|
B:HIS272
|
4.5
|
23.0
|
1.0
|
C1
|
B:XLS503
|
4.6
|
38.7
|
1.0
|
ND1
|
B:HIS272
|
4.6
|
20.9
|
1.0
|
ND2
|
B:ASN267
|
4.6
|
17.8
|
1.0
|
C5
|
B:XLS503
|
4.8
|
33.8
|
1.0
|
CA
|
B:CA502
|
4.8
|
21.1
|
1.0
|
O3
|
B:XLS503
|
4.9
|
40.9
|
1.0
|
|
Calcium binding site 4 out
of 8 in 6t8f
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Calcium Binding Sites List in 6t8f
Calcium binding site 4 out
of 8 in the Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca502
b:21.1
occ:1.00
|
O
|
B:HOH637
|
1.9
|
26.3
|
1.0
|
OE2
|
B:GLU269
|
1.9
|
23.6
|
1.0
|
OD2
|
B:ASP308
|
1.9
|
30.1
|
1.0
|
OD1
|
B:ASP310
|
1.9
|
22.5
|
1.0
|
CG
|
B:ASP308
|
2.6
|
30.4
|
1.0
|
OD1
|
B:ASP308
|
2.6
|
33.5
|
1.0
|
CD
|
B:GLU269
|
2.9
|
22.4
|
1.0
|
CG
|
B:ASP310
|
2.9
|
22.0
|
1.0
|
NE2
|
B:HIS272
|
3.0
|
23.0
|
1.0
|
OE1
|
B:GLU269
|
3.2
|
23.9
|
1.0
|
OD2
|
B:ASP310
|
3.2
|
24.7
|
1.0
|
O
|
B:HOH610
|
3.5
|
27.9
|
1.0
|
CD2
|
B:HIS272
|
3.6
|
20.2
|
1.0
|
ND2
|
B:ASN299
|
3.8
|
13.9
|
1.0
|
O2
|
B:XLS503
|
3.9
|
34.5
|
1.0
|
CB
|
B:ASP308
|
4.0
|
25.8
|
1.0
|
CE1
|
B:HIS272
|
4.1
|
22.3
|
1.0
|
CG
|
B:GLU269
|
4.2
|
20.6
|
1.0
|
OD2
|
B:ASP340
|
4.3
|
19.9
|
1.0
|
CB
|
B:ASP310
|
4.3
|
20.8
|
1.0
|
CG
|
B:ASP340
|
4.5
|
19.6
|
1.0
|
C1
|
B:XLS503
|
4.6
|
38.7
|
1.0
|
CB
|
B:ASN271
|
4.6
|
17.3
|
1.0
|
OD1
|
B:ASP340
|
4.6
|
21.8
|
1.0
|
O
|
B:ASP340
|
4.7
|
15.9
|
1.0
|
CG
|
B:HIS272
|
4.8
|
20.0
|
1.0
|
C2
|
B:XLS503
|
4.8
|
38.2
|
1.0
|
CA
|
B:ASP310
|
4.8
|
19.9
|
1.0
|
CA
|
B:CA501
|
4.8
|
16.5
|
1.0
|
ND2
|
B:ASN271
|
4.9
|
15.5
|
1.0
|
O
|
B:HOH603
|
4.9
|
32.3
|
1.0
|
O1
|
B:XLS503
|
4.9
|
43.1
|
1.0
|
N
|
B:ASP310
|
5.0
|
18.3
|
1.0
|
ND1
|
B:HIS272
|
5.0
|
20.9
|
1.0
|
|
Calcium binding site 5 out
of 8 in 6t8f
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Calcium Binding Sites List in 6t8f
Calcium binding site 5 out
of 8 in the Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 5 of Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Ca501
b:13.5
occ:1.00
|
O2
|
C:XLS503
|
2.0
|
30.9
|
1.0
|
OD2
|
C:ASP297
|
2.1
|
20.6
|
1.0
|
OE2
|
C:GLU233
|
2.1
|
19.1
|
1.0
|
OE1
|
C:GLU269
|
2.2
|
22.2
|
1.0
|
OD2
|
C:ASP340
|
2.2
|
18.2
|
1.0
|
O4
|
C:XLS503
|
2.2
|
27.6
|
1.0
|
CD
|
C:GLU233
|
2.9
|
17.7
|
1.0
|
OE1
|
C:GLU233
|
3.1
|
20.1
|
1.0
|
C4
|
C:XLS503
|
3.2
|
30.9
|
1.0
|
CG
|
C:ASP297
|
3.2
|
18.9
|
1.0
|
C2
|
C:XLS503
|
3.2
|
32.1
|
1.0
|
CG
|
C:ASP340
|
3.3
|
17.1
|
1.0
|
CD
|
C:GLU269
|
3.4
|
18.9
|
1.0
|
C3
|
C:XLS503
|
3.7
|
33.1
|
1.0
|
CB
|
C:ASP297
|
3.8
|
18.0
|
1.0
|
CB
|
C:ASP340
|
3.8
|
16.5
|
1.0
|
O
|
C:HOH732
|
3.8
|
20.8
|
1.0
|
CE1
|
C:HIS272
|
4.0
|
16.4
|
1.0
|
CG
|
C:GLU269
|
4.1
|
17.9
|
1.0
|
CB
|
C:GLU269
|
4.2
|
16.2
|
1.0
|
OD1
|
C:ASP297
|
4.3
|
19.9
|
1.0
|
CG
|
C:GLU233
|
4.3
|
17.2
|
1.0
|
OE2
|
C:GLU269
|
4.3
|
21.1
|
1.0
|
C1
|
C:XLS503
|
4.4
|
33.7
|
1.0
|
OD1
|
C:ASP340
|
4.4
|
17.0
|
1.0
|
NE2
|
C:HIS272
|
4.5
|
17.5
|
1.0
|
C5
|
C:XLS503
|
4.6
|
31.2
|
1.0
|
ND2
|
C:ASN267
|
4.6
|
14.2
|
1.0
|
ND1
|
C:HIS272
|
4.7
|
16.3
|
1.0
|
O3
|
C:XLS503
|
4.7
|
37.9
|
1.0
|
CA
|
C:CA502
|
4.8
|
18.6
|
1.0
|
|
Calcium binding site 6 out
of 8 in 6t8f
Go back to
Calcium Binding Sites List in 6t8f
Calcium binding site 6 out
of 8 in the Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 6 of Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Ca502
b:18.6
occ:1.00
|
OE2
|
C:GLU269
|
1.9
|
21.1
|
1.0
|
O
|
C:HOH732
|
1.9
|
20.8
|
1.0
|
OD1
|
C:ASP310
|
1.9
|
22.1
|
1.0
|
OD2
|
C:ASP308
|
2.0
|
26.5
|
1.0
|
CG
|
C:ASP308
|
2.6
|
25.6
|
1.0
|
OD1
|
C:ASP308
|
2.7
|
31.3
|
1.0
|
CD
|
C:GLU269
|
2.8
|
18.9
|
1.0
|
CG
|
C:ASP310
|
2.9
|
20.6
|
1.0
|
NE2
|
C:HIS272
|
3.0
|
17.5
|
1.0
|
OE1
|
C:GLU269
|
3.1
|
22.2
|
1.0
|
OD2
|
C:ASP310
|
3.2
|
21.9
|
1.0
|
CD2
|
C:HIS272
|
3.6
|
16.7
|
1.0
|
ND2
|
C:ASN299
|
3.7
|
13.8
|
1.0
|
O
|
C:HOH714
|
3.8
|
24.3
|
1.0
|
O2
|
C:XLS503
|
3.9
|
30.9
|
1.0
|
CB
|
C:ASP308
|
4.1
|
22.9
|
1.0
|
CE1
|
C:HIS272
|
4.1
|
16.4
|
1.0
|
CG
|
C:GLU269
|
4.2
|
17.9
|
1.0
|
OD2
|
C:ASP340
|
4.3
|
18.2
|
1.0
|
CB
|
C:ASP310
|
4.3
|
18.8
|
1.0
|
C1
|
C:XLS503
|
4.3
|
33.7
|
1.0
|
CG
|
C:ASP340
|
4.5
|
17.1
|
1.0
|
CB
|
C:ASN271
|
4.6
|
16.0
|
1.0
|
OD1
|
C:ASP340
|
4.6
|
17.0
|
1.0
|
C2
|
C:XLS503
|
4.7
|
32.1
|
1.0
|
O
|
C:ASP340
|
4.7
|
16.0
|
1.0
|
CG
|
C:HIS272
|
4.7
|
16.3
|
1.0
|
CA
|
C:ASP310
|
4.8
|
17.3
|
1.0
|
CA
|
C:CA501
|
4.8
|
13.5
|
1.0
|
ND1
|
C:HIS272
|
5.0
|
16.3
|
1.0
|
OD1
|
C:ASN271
|
5.0
|
16.9
|
1.0
|
N
|
C:ASP310
|
5.0
|
17.1
|
1.0
|
|
Calcium binding site 7 out
of 8 in 6t8f
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Calcium Binding Sites List in 6t8f
Calcium binding site 7 out
of 8 in the Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 7 of Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Ca501
b:15.3
occ:1.00
|
OD2
|
D:ASP297
|
2.1
|
19.8
|
1.0
|
OE2
|
D:GLU233
|
2.1
|
19.4
|
1.0
|
OE1
|
D:GLU269
|
2.1
|
21.4
|
1.0
|
OD2
|
D:ASP340
|
2.1
|
19.9
|
1.0
|
O2
|
D:XLS503
|
2.2
|
32.8
|
1.0
|
O4
|
D:XLS503
|
2.3
|
26.0
|
1.0
|
CD
|
D:GLU233
|
2.9
|
18.3
|
1.0
|
OE1
|
D:GLU233
|
3.1
|
19.4
|
1.0
|
C2
|
D:XLS503
|
3.2
|
34.1
|
1.0
|
CG
|
D:ASP297
|
3.2
|
18.7
|
1.0
|
CG
|
D:ASP340
|
3.2
|
19.0
|
1.0
|
C4
|
D:XLS503
|
3.3
|
31.4
|
1.0
|
CD
|
D:GLU269
|
3.3
|
20.4
|
1.0
|
C3
|
D:XLS503
|
3.6
|
33.8
|
1.0
|
O
|
D:HOH604
|
3.7
|
20.2
|
1.0
|
CB
|
D:ASP340
|
3.7
|
17.5
|
1.0
|
CB
|
D:ASP297
|
3.8
|
17.0
|
1.0
|
O3
|
D:XLS503
|
3.8
|
40.0
|
1.0
|
CE1
|
D:HIS272
|
4.0
|
18.8
|
1.0
|
CG
|
D:GLU269
|
4.1
|
18.6
|
1.0
|
CB
|
D:GLU269
|
4.2
|
17.2
|
1.0
|
OE2
|
D:GLU269
|
4.3
|
21.1
|
1.0
|
OD1
|
D:ASP297
|
4.3
|
17.4
|
1.0
|
OD1
|
D:ASP340
|
4.3
|
20.3
|
1.0
|
CG
|
D:GLU233
|
4.3
|
17.7
|
1.0
|
NE2
|
D:HIS272
|
4.5
|
20.0
|
1.0
|
C1
|
D:XLS503
|
4.6
|
34.8
|
1.0
|
ND1
|
D:HIS272
|
4.6
|
18.5
|
1.0
|
ND2
|
D:ASN267
|
4.7
|
18.3
|
1.0
|
C5
|
D:XLS503
|
4.7
|
31.4
|
1.0
|
CA
|
D:CA502
|
4.8
|
20.0
|
1.0
|
|
Calcium binding site 8 out
of 8 in 6t8f
Go back to
Calcium Binding Sites List in 6t8f
Calcium binding site 8 out
of 8 in the Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 8 of Crystal Structure of Mutant Xylose Isomerase (V270A/A273G) From Piromyces E2 Grown in Yeast, in Complex with Xylose within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Ca502
b:20.0
occ:1.00
|
O
|
D:HOH604
|
1.8
|
20.2
|
1.0
|
OD2
|
D:ASP308
|
1.9
|
32.3
|
1.0
|
OD1
|
D:ASP310
|
2.0
|
21.5
|
1.0
|
OE2
|
D:GLU269
|
2.0
|
21.1
|
1.0
|
CG
|
D:ASP308
|
2.6
|
27.5
|
1.0
|
OD1
|
D:ASP308
|
2.6
|
34.2
|
1.0
|
CD
|
D:GLU269
|
2.9
|
20.4
|
1.0
|
CG
|
D:ASP310
|
2.9
|
20.4
|
1.0
|
NE2
|
D:HIS272
|
3.0
|
20.0
|
1.0
|
OE1
|
D:GLU269
|
3.2
|
21.4
|
1.0
|
OD2
|
D:ASP310
|
3.2
|
23.4
|
1.0
|
CD2
|
D:HIS272
|
3.6
|
18.5
|
1.0
|
O
|
D:HOH806
|
3.7
|
27.6
|
1.0
|
O2
|
D:XLS503
|
3.7
|
32.8
|
1.0
|
ND2
|
D:ASN299
|
3.8
|
14.5
|
1.0
|
CB
|
D:ASP308
|
4.0
|
24.5
|
1.0
|
CE1
|
D:HIS272
|
4.1
|
18.8
|
1.0
|
CG
|
D:GLU269
|
4.3
|
18.6
|
1.0
|
CB
|
D:ASP310
|
4.3
|
18.8
|
1.0
|
OD2
|
D:ASP340
|
4.3
|
19.9
|
1.0
|
CG
|
D:ASP340
|
4.6
|
19.0
|
1.0
|
CB
|
D:ASN271
|
4.6
|
17.2
|
1.0
|
OD1
|
D:ASP340
|
4.7
|
20.3
|
1.0
|
O1
|
D:XLS503
|
4.7
|
36.0
|
1.0
|
C2
|
D:XLS503
|
4.7
|
34.1
|
1.0
|
O
|
D:ASP340
|
4.7
|
15.3
|
1.0
|
CG
|
D:HIS272
|
4.8
|
18.2
|
1.0
|
C1
|
D:XLS503
|
4.8
|
34.8
|
1.0
|
CA
|
D:ASP310
|
4.8
|
17.2
|
1.0
|
CA
|
D:CA501
|
4.8
|
15.3
|
1.0
|
OD1
|
D:ASN271
|
5.0
|
17.1
|
1.0
|
ND1
|
D:HIS272
|
5.0
|
18.5
|
1.0
|
N
|
D:ASP310
|
5.0
|
15.9
|
1.0
|
CA
|
D:ASP308
|
5.0
|
21.2
|
1.0
|
|
Reference:
M.Lee,
H.J.Rozeboom,
E.Keuning,
P.De Waal,
D.B.Janssen.
Disconnection Between in Vitro Catalytic Properties of Engineered Xylose Isomerases and Rates of Xylose Metabolism in Yeast Biotechnol Biofuels 2020.
ISSN: ESSN 1754-6834
DOI: 10.1186/S13068-019-1643-0
Page generated: Tue Jul 16 15:12:20 2024
|