Calcium in PDB 6u7j: Uncultured Clostridium Sp. Beta-Glucuronidase

All present enzymatic activity of Uncultured Clostridium Sp. Beta-Glucuronidase:
3.2.1.31;

The structure of Uncultured Clostridium Sp. Beta-Glucuronidase, PDB code: 6u7j was solved by S.M.Ervin, M.R.Redinbo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.31 / 2.20
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	74.941, 105.655, 189.566, 90.00, 94.60, 90.00
R / Rfree (%)	16.5 / 20.7

The binding sites of Calcium atom in the Uncultured Clostridium Sp. Beta-Glucuronidase (pdb code 6u7j). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Uncultured Clostridium Sp. Beta-Glucuronidase, PDB code: 6u7j:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Uncultured Clostridium Sp. Beta-Glucuronidase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Uncultured Clostridium Sp. Beta-Glucuronidase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca601 b:55.9 occ:1.00 OE1 A:GLU347 3.2 41.6 1.0 OE2 A:GLU347 3.7 43.6 1.0 CD A:GLU347 3.8 42.7 1.0 O A:HOH729 3.8 33.7 1.0 CG A:GLU346 3.9 42.3 1.0 N A:GLU346 3.9 31.4 1.0 CD A:ARG186 4.0 32.1 1.0 CD A:PRO187 4.1 29.2 1.0 CB A:ARG186 4.1 29.1 1.0 CA A:GLY99 4.2 30.8 1.0 NH1 A:ARG97 4.2 44.9 1.0 NH1 A:ARG189 4.3 50.6 1.0 CG A:ARG186 4.3 34.0 1.0 CB A:GLU346 4.4 36.5 1.0 CB A:ALA345 4.4 31.5 1.0 CE1 A:PHE12 4.5 34.3 1.0 OE2 A:GLU346 4.5 47.7 1.0 CA A:ALA345 4.5 32.1 1.0 CD A:GLU346 4.7 47.0 1.0 CA A:GLU346 4.8 34.4 1.0 C A:ALA345 4.8 35.3 1.0 NH2 A:ARG189 4.8 52.1 1.0 CG A:PRO187 4.9 32.8 1.0 C A:GLY99 4.9 30.8 1.0 N A:GLU347 5.0 30.3 1.0 CZ A:ARG189 5.0 52.0 1.0

Calcium binding site 2 out of 4 in the Uncultured Clostridium Sp. Beta-Glucuronidase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Uncultured Clostridium Sp. Beta-Glucuronidase within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca601 b:58.3 occ:1.00 O B:HOH846 2.2 42.2 1.0 OE1 B:GLU347 3.0 41.3 1.0 OE2 B:GLU347 3.5 47.1 1.0 O B:HOH907 3.6 44.5 1.0 CD B:GLU347 3.7 43.5 1.0 O B:HOH709 3.8 51.7 1.0 N B:GLU346 4.0 32.1 1.0 CD B:PRO187 4.1 33.8 1.0 CB B:ARG186 4.2 30.8 1.0 O B:HOH741 4.2 31.5 1.0 CD B:ARG186 4.2 31.3 1.0 CA B:GLY99 4.3 32.5 1.0 CG B:GLU346 4.4 37.7 1.0 CB B:GLU346 4.4 34.8 1.0 OE2 B:GLU346 4.4 44.0 1.0 CB B:ALA345 4.4 29.9 1.0 CE1 B:PHE12 4.5 35.1 1.0 CG B:ARG186 4.5 31.0 1.0 CA B:ALA345 4.7 32.3 1.0 CG B:PRO187 4.7 34.3 1.0 CA B:GLU346 4.8 32.5 1.0 C B:ALA345 4.8 33.8 1.0 N B:GLU347 4.8 33.4 1.0 CD B:GLU346 4.9 44.7 1.0 N B:PRO187 4.9 31.2 1.0

Calcium binding site 3 out of 4 in the Uncultured Clostridium Sp. Beta-Glucuronidase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Uncultured Clostridium Sp. Beta-Glucuronidase within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca601 b:59.2 occ:1.00 OE1 C:GLU347 3.0 40.5 1.0 OE2 C:GLU347 3.6 44.5 1.0 CD C:GLU347 3.7 41.1 1.0 OE2 C:GLU346 3.8 44.0 1.0 N C:GLU346 3.9 31.7 1.0 CG C:GLU346 3.9 39.5 1.0 O C:HOH794 4.0 30.5 1.0 NH1 C:ARG189 4.1 48.2 1.0 CB C:GLU346 4.2 36.5 1.0 CA C:GLY99 4.2 25.5 1.0 CD C:ARG186 4.2 28.7 1.0 CD C:GLU346 4.3 43.7 1.0 CD C:PRO187 4.3 28.7 1.0 CB C:ARG186 4.3 30.9 1.0 CB C:ALA345 4.4 28.1 1.0 CE1 C:PHE12 4.5 37.6 1.0 CG C:ARG186 4.5 25.3 1.0 CA C:ALA345 4.6 30.4 1.0 CA C:GLU346 4.6 31.2 1.0 C C:ALA345 4.7 32.6 1.0 N C:GLU347 4.8 34.0 1.0 C C:GLY99 5.0 29.8 1.0

Calcium binding site 4 out of 4 in the Uncultured Clostridium Sp. Beta-Glucuronidase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Uncultured Clostridium Sp. Beta-Glucuronidase within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca601 b:55.2 occ:1.00 OE1 D:GLU347 2.9 38.8 1.0 O D:HOH903 3.6 45.1 1.0 CD D:GLU347 3.8 41.6 1.0 OE2 D:GLU347 3.9 42.8 1.0 N D:GLU346 4.0 30.4 1.0 O D:HOH717 4.0 28.1 1.0 CD D:PRO187 4.1 27.6 1.0 CA D:GLY99 4.2 28.1 1.0 OE2 D:GLU346 4.2 46.8 1.0 CG D:GLU346 4.2 35.5 1.0 CD D:ARG186 4.2 27.9 1.0 CB D:ARG186 4.2 30.8 1.0 CB D:GLU346 4.4 34.8 1.0 CB D:ALA345 4.4 29.6 1.0 CG D:ARG186 4.5 30.4 1.0 CE1 D:PHE12 4.5 34.7 1.0 NH1 D:ARG97 4.5 49.7 1.0 CA D:ALA345 4.6 30.0 1.0 CD D:GLU346 4.6 43.6 1.0 CA D:GLU346 4.7 31.7 1.0 C D:ALA345 4.8 32.9 1.0 N D:GLU347 4.8 33.2 1.0 CG D:PRO187 4.9 31.1 1.0 N D:PRO187 4.9 29.4 1.0 C D:GLY99 4.9 31.8 1.0

S.M.Ervin, M.R.Redinbo. Uncultured Clostridium Sp. Beta-Glucuronidase To Be Published.