Atomistry » Calcium » PDB 6x6m-6xqn » 6xqf
Atomistry »
  Calcium »
    PDB 6x6m-6xqn »
      6xqf »

Calcium in PDB 6xqf: Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with 1,3-Beta-D- Cellotriosyl-Glucose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site

Enzymatic activity of Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with 1,3-Beta-D- Cellotriosyl-Glucose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site

All present enzymatic activity of Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with 1,3-Beta-D- Cellotriosyl-Glucose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site:
3.2.1.39;

Protein crystallography data

The structure of Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with 1,3-Beta-D- Cellotriosyl-Glucose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site, PDB code: 6xqf was solved by M.V.Liberato, F.Squina, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 45.72 / 1.58
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 38.692, 49.868, 114.474, 90, 90, 90
R / Rfree (%) 17.1 / 20.9

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with 1,3-Beta-D- Cellotriosyl-Glucose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site (pdb code 6xqf). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with 1,3-Beta-D- Cellotriosyl-Glucose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site, PDB code: 6xqf:

Calcium binding site 1 out of 1 in 6xqf

Go back to Calcium Binding Sites List in 6xqf
Calcium binding site 1 out of 1 in the Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with 1,3-Beta-D- Cellotriosyl-Glucose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with 1,3-Beta-D- Cellotriosyl-Glucose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca301

b:7.5
occ:1.00
 O A:GLY72 2.3 8.4 1.0
O A:GLU28 2.3 8.0 1.0
O A:ASP258 2.4 7.5 1.0
OD1 A:ASP258 2.4 8.6 1.0
O A:HOH496 2.4 7.3 1.0
O A:HOH519 2.5 10.4 1.0
O A:HOH554 2.6 10.6 1.0
C A:GLY72 3.4 9.0 1.0
C A:ASP258 3.4 7.2 1.0
C A:GLU28 3.5 8.0 1.0
CG A:ASP258 3.6 8.5 1.0
CA A:GLY72 3.8 10.1 1.0
CA A:ASP258 3.9 7.6 1.0
CA A:GLU28 4.2 7.9 1.0
CB A:ASP258 4.3 7.9 1.0
O A:HOH671 4.3 22.7 1.0
O A:HOH622 4.4 18.2 1.0
OD2 A:ASP258 4.5 8.8 1.0
N A:PHE29 4.5 7.9 1.0
CB A:GLU28 4.5 8.6 1.0
CB A:PHE29 4.5 7.6 1.0
N A:ASN73 4.5 8.5 1.0
O A:PHE29 4.5 9.0 1.0
N A:TYR259 4.5 6.9 1.0
C A:PHE29 4.6 8.9 1.0
OE1 A:GLU28 4.7 8.3 1.0
OD1 A:ASN30 4.7 10.8 1.0
CA A:PHE29 4.7 8.1 1.0
OD1 A:ASN73 4.8 9.3 1.0
CB A:TYR259 4.8 7.1 1.0
O A:HOH751 4.8 14.1 1.0
CA A:TYR259 4.9 6.7 1.0
CA A:ASN73 4.9 8.0 1.0

Reference:

M.V.Liberato, E.T.Prates, A.Bernardes, J.Fattori, G.Ematsu, M.Chinaglia, E.R.M.Gomes, A.C.M.Figueira, I.Polikarpov, M.Skaf, F.Squina. A Structural Basis of the Cleavage Mechanism of Non-Specific Endo-Beta-1,3(4)-Glucanase From Family GH16. To Be Published.
Page generated: Tue Jul 16 18:05:22 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy