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Calcium in PDB 6xql: Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with Cellohexaose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site

Enzymatic activity of Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with Cellohexaose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site

All present enzymatic activity of Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with Cellohexaose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site:
3.2.1.39;

Protein crystallography data

The structure of Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with Cellohexaose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site, PDB code: 6xql was solved by M.V.Liberato, F.Squina, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 45.02 / 1.97
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 38.4, 49.006, 113.995, 90, 90, 90
R / Rfree (%) 17 / 23.9

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with Cellohexaose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site (pdb code 6xql). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with Cellohexaose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site, PDB code: 6xql:

Calcium binding site 1 out of 1 in 6xql

Go back to Calcium Binding Sites List in 6xql
Calcium binding site 1 out of 1 in the Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with Cellohexaose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Sclam E144S Mutant, A Non-Specific Endo-Beta-1, 3(4)-Glucanase From Family GH16, Co-Crystallized with Cellohexaose, Presenting A 1,3-Beta-D-Cellobiosyl-Glucose at Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca301

b:22.3
occ:1.00
 O A:HOH507 2.3 17.1 1.0
O A:GLU28 2.4 20.8 1.0
O A:GLY72 2.4 21.7 1.0
O A:ASP258 2.4 20.2 1.0
OD1 A:ASP258 2.4 18.5 1.0
O A:HOH422 2.6 25.7 1.0
O A:HOH575 2.6 20.7 1.0
C A:ASP258 3.4 19.5 1.0
C A:GLY72 3.4 22.3 1.0
C A:GLU28 3.5 21.4 1.0
CG A:ASP258 3.6 18.6 1.0
CA A:GLY72 3.8 23.8 1.0
CA A:ASP258 3.9 18.6 1.0
CA A:GLU28 4.2 21.4 1.0
CB A:ASP258 4.4 18.0 1.0
N A:TYR259 4.5 20.1 1.0
O A:HOH645 4.5 31.9 1.0
OD2 A:ASP258 4.5 19.3 1.0
N A:PHE29 4.6 22.2 1.0
O A:PHE29 4.6 25.0 1.0
N A:ASN73 4.6 22.9 1.0
OD1 A:ASN73 4.6 23.0 1.0
OD1 A:ASN30 4.6 23.6 1.0
C A:PHE29 4.6 23.6 1.0
CB A:GLU28 4.6 22.2 1.0
CB A:PHE29 4.6 22.0 1.0
OE1 A:GLU28 4.7 20.1 1.0
CB A:TYR259 4.7 21.0 1.0
O A:HOH604 4.8 39.7 1.0
CA A:PHE29 4.9 22.8 1.0
CA A:TYR259 4.9 19.9 1.0
CA A:ASN73 5.0 22.6 1.0

Reference:

M.V.Liberato, E.T.Prates, A.Bernardes, J.Fattori, G.Ematsu, M.Chinaglia, E.R.M.Gomes, A.C.M.Figueira, I.Polikarpov, M.Skaf, F.Squina. A Structural Basis of the Cleavage Mechanism of Non-Specific Endo-Beta-1,3(4)-Glucanase From Family GH16. To Be Published.
Page generated: Tue Jul 16 18:05:50 2024

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