Calcium in PDB 6xwd: 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif

The structure of 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif, PDB code: 6xwd was solved by S.Kiehstaller, S.Hennig, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.37 / 1.60
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	82.000, 111.600, 62.400, 90.00, 90.00, 90.00
R / Rfree (%)	16.6 / 19.6

The structure of 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Sodium	(Na)	2 atoms

The binding sites of Calcium atom in the 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif (pdb code 6xwd). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif, PDB code: 6xwd:

Calcium binding site 1 out of 1 in the 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of 14-3-3 Sigma Bound to Canonical Mono-Phosphorylated Aminopeptidase N (Apn, CD13) Binding Motif within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca303 b:24.6 occ:1.00 O A:HOH730 3.0 28.2 1.0 O A:HOH665 3.0 24.7 1.0 CD A:LYS87 3.8 25.3 1.0 CA A:TYR84 3.9 16.5 1.0 O A:GLU83 3.9 16.4 1.0 CB A:LYS87 4.0 17.8 1.0 C A:GLU83 4.1 16.8 1.0 N A:TYR84 4.1 15.7 1.0 CD1 A:TYR84 4.1 15.3 1.0 CB A:TYR84 4.3 16.6 1.0 CG A:LYS87 4.4 21.9 1.0 CB A:GLU83 4.4 16.9 1.0 O A:HOH504 4.5 27.0 1.0 CG A:TYR84 4.7 14.9 1.0 O A:HOH725 4.8 49.1 1.0 CA A:GLU83 4.9 16.2 1.0 CE A:LYS87 4.9 40.2 1.0

S.Kiehstaller, C.Ottmann, S.Hennig. Mmp Activation Associated Aminopeptidase N Reveals A Bivalent 14-3-3 Binding Motif. J.Biol.Chem. 2020.
ISSN: ESSN 1083-351X
PubMed: 33109610
DOI: 10.1074/JBC.RA120.014708