Calcium in PDB 6y98: Crystal Structure of Subtype-Switched Epithelial Adhesin 9 to 1 A Domain (EPA9-CBL2EPA1) From Candida Glabrata in Complex with Beta- Lactose

The structure of Crystal Structure of Subtype-Switched Epithelial Adhesin 9 to 1 A Domain (EPA9-CBL2EPA1) From Candida Glabrata in Complex with Beta- Lactose, PDB code: 6y98 was solved by D.Hoffmann, R.Diderrich, M.Kock, S.Friederichs, V.Reithofer, L.-O.Essen, H.-U.Moesch, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.63 / 2.80
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	65.289, 65.289, 121.877, 90.00, 90.00, 120.00
R / Rfree (%)	20.4 / 24.8

The binding sites of Calcium atom in the Crystal Structure of Subtype-Switched Epithelial Adhesin 9 to 1 A Domain (EPA9-CBL2EPA1) From Candida Glabrata in Complex with Beta- Lactose (pdb code 6y98). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Subtype-Switched Epithelial Adhesin 9 to 1 A Domain (EPA9-CBL2EPA1) From Candida Glabrata in Complex with Beta- Lactose, PDB code: 6y98:

Calcium binding site 1 out of 1 in the Crystal Structure of Subtype-Switched Epithelial Adhesin 9 to 1 A Domain (EPA9-CBL2EPA1) From Candida Glabrata in Complex with Beta- Lactose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Subtype-Switched Epithelial Adhesin 9 to 1 A Domain (EPA9-CBL2EPA1) From Candida Glabrata in Complex with Beta- Lactose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:0.5 occ:1.00 OD1 A:ASP196 2.3 0.8 1.0 O A:GLU258 2.3 0.9 1.0 HO4 B:GAL2 2.4 0.5 1.0 OD2 A:ASP197 2.4 0.8 1.0 O3 B:GAL2 2.5 0.2 1.0 OD1 A:ASN256 2.5 0.3 1.0 O A:ASP260 2.6 98.7 1.0 O4 B:GAL2 2.7 0.4 1.0 HO3 B:GAL2 2.9 0.7 1.0 CG A:ASP197 3.2 0.6 1.0 H4 B:GAL2 3.3 0.2 1.0 CG A:ASP196 3.3 0.4 1.0 C4 B:GAL2 3.4 0.4 1.0 C3 B:GAL2 3.5 0.1 1.0 C A:GLU258 3.5 0.1 1.0 OD1 A:ASP197 3.5 0.5 1.0 OD2 A:ASP196 3.5 0.3 1.0 CG A:ASN256 3.6 0.2 1.0 C A:ASP260 3.7 0.5 1.0 H2 B:GAL2 3.8 0.5 1.0 N A:GLU258 3.9 0.6 1.0 C A:TYR259 4.1 0.1 1.0 O A:TYR259 4.1 0.4 1.0 ND2 A:ASN256 4.2 0.1 1.0 N A:ASP260 4.2 0.5 1.0 H3 B:GAL2 4.2 0.4 1.0 C2 B:GAL2 4.2 0.4 1.0 CA A:GLU258 4.3 0.4 1.0 N A:ASP197 4.4 1.0 1.0 N A:TYR259 4.4 0.0 1.0 C A:ASP196 4.5 0.9 1.0 CB A:ASP197 4.5 90.3 1.0 CA A:ASP260 4.5 0.0 1.0 N A:ARG257 4.5 0.5 1.0 CG A:ARG257 4.5 0.9 1.0 O A:ASP196 4.6 0.3 1.0 CA A:TYR259 4.6 0.5 1.0 CB A:ASP196 4.6 0.1 1.0 CB A:TYR259 4.7 0.6 1.0 N A:SER261 4.8 0.3 1.0 CA A:ASP197 4.8 0.4 1.0 C5 B:GAL2 4.9 0.6 1.0 CB A:ASN256 4.9 96.1 1.0 O2 B:GAL2 4.9 0.9 1.0 CB A:ASP260 4.9 0.3 1.0 CB A:GLU258 4.9 94.4 1.0 CA A:SER261 5.0 0.8 1.0 C A:ARG257 5.0 93.9 1.0 CA A:ASN256 5.0 92.9 1.0 CA A:ASP196 5.0 0.8 1.0

D.Hoffmann, R.Diderrich, V.Reithofer, S.Friederichs, M.Kock, L.O.Essen, H.U.Mosch. Functional Reprogramming Ofcandida Glabrataepithelial Adhesins: the Role of Conserved and Variable Structural Motifs in Ligand Binding. J.Biol.Chem. V. 295 12512 2020.
ISSN: ESSN 1083-351X
PubMed: 32669365
DOI: 10.1074/JBC.RA120.013968