Calcium in PDB 6zsn: Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated)

The structure of Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated), PDB code: 6zsn was solved by R.Janowski, J.P.Fuenzalida-Werner, K.Mishra, A.C.Stiel, D.Niessing, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.61 / 2.60
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	118.97, 118.97, 96.23, 90, 90, 90
R / Rfree (%)	19.1 / 25.9

The structure of Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated) also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Calcium atom in the Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated) (pdb code 6zsn). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated), PDB code: 6zsn:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca507 b:81.7 occ:1.00 OD1 A:ASP436 2.2 78.6 1.0 OD1 A:ASP434 2.4 78.6 1.0 OD1 A:ASP432 2.4 62.4 1.0 O A:GLN438 2.4 63.5 1.0 OE1 A:GLU443 2.5 76.4 1.0 OE2 A:GLU443 2.6 73.3 1.0 CD A:GLU443 2.9 81.5 1.0 CG A:ASP436 3.0 77.0 1.0 OD2 A:ASP436 3.3 81.3 1.0 CG A:ASP434 3.4 86.1 1.0 CG A:ASP432 3.5 76.1 1.0 C A:GLN438 3.5 68.0 1.0 OD2 A:ASP434 3.7 82.8 1.0 CB A:ASP436 4.2 77.7 1.0 N A:ASP436 4.2 72.7 1.0 N A:GLN438 4.2 62.1 1.0 OD2 A:ASP432 4.2 70.0 1.0 N A:VAL439 4.3 62.0 1.0 N A:ASN440 4.3 60.2 1.0 CA A:GLN438 4.4 65.9 1.0 CB A:ASP432 4.4 83.2 1.0 CG A:GLU443 4.4 83.1 1.0 CA A:VAL439 4.5 58.7 1.0 CA A:ASP432 4.5 86.7 1.0 N A:GLY435 4.5 73.9 1.0 N A:ASP434 4.6 93.4 1.0 CA A:ASP436 4.7 72.5 1.0 CB A:ASP434 4.7 87.3 1.0 CG A:ASN440 4.8 76.8 1.0 CB A:GLN438 4.8 67.5 1.0 N A:ILE433 4.8 78.7 1.0 C A:VAL439 4.9 57.4 1.0 ND2 A:ASN440 4.9 75.0 1.0 N A:GLY437 4.9 76.3 1.0 C A:ASP432 4.9 77.7 1.0 CA A:ASP434 5.0 88.3 1.0 CB A:ASN440 5.0 71.9 1.0 OD1 A:ASN440 5.0 79.7 1.0

Calcium binding site 2 out of 4 in the Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca508 b:47.8 occ:1.00 OD1 A:ASP325 2.3 51.3 1.0 OE1 A:GLU334 2.3 61.1 1.0 OD1 A:ASP327 2.4 48.9 1.0 OD1 A:ASP323 2.4 44.4 1.0 O A:THR329 2.4 45.0 1.0 OE2 A:GLU334 2.7 48.7 1.0 CD A:GLU334 2.9 56.3 1.0 CG A:ASP327 3.2 43.4 1.0 CG A:ASP325 3.3 44.0 1.0 C A:THR329 3.6 42.5 1.0 CG A:ASP323 3.6 49.6 1.0 OD2 A:ASP327 3.6 43.3 1.0 OD2 A:ASP325 3.7 41.0 1.0 CA A:ASP323 4.3 52.1 1.0 N A:THR329 4.3 42.9 1.0 N A:ASP327 4.3 42.2 1.0 CG2 A:THR329 4.4 43.9 1.0 CB A:ASP323 4.4 51.7 1.0 CG A:GLU334 4.4 51.4 1.0 CB A:ASP327 4.4 45.1 1.0 CA A:THR329 4.5 45.3 1.0 N A:ILE330 4.5 41.5 1.0 CB A:ASP325 4.5 43.0 1.0 OD2 A:ASP323 4.5 42.3 1.0 N A:ASP325 4.6 51.4 1.0 CA A:ILE330 4.6 41.7 1.0 N A:GLY326 4.7 43.7 1.0 C A:ASP323 4.7 54.0 1.0 CA A:ASP327 4.8 45.0 1.0 N A:LYS324 4.8 52.5 1.0 N A:GLY328 4.9 42.5 1.0 CA A:ASP325 4.9 46.1 1.0 O A:HOH613 4.9 48.0 1.0 N A:THR331 4.9 41.9 1.0 C A:ASP325 5.0 47.0 1.0

Calcium binding site 3 out of 4 in the Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca509 b:49.0 occ:1.00 OD1 A:ASP359 2.2 44.8 1.0 OE1 A:GLU370 2.4 43.2 1.0 OD1 A:ASP361 2.4 48.5 1.0 O A:THR365 2.5 43.2 1.0 OD2 A:ASP367 2.6 60.1 1.0 OE2 A:GLU370 2.7 48.5 1.0 OD1 A:ASP363 2.7 48.9 1.0 CD A:GLU370 2.9 47.4 1.0 CG A:ASP361 3.2 54.0 1.0 CG A:ASP363 3.3 48.5 1.0 CG A:ASP359 3.4 44.2 1.0 OD2 A:ASP361 3.5 56.0 1.0 C A:THR365 3.6 40.8 1.0 OD2 A:ASP363 3.7 62.4 1.0 CG A:ASP367 3.8 58.1 1.0 OG1 A:THR365 4.0 39.4 1.0 N A:ASP363 4.1 48.8 1.0 OD2 A:ASP359 4.1 47.8 1.0 N A:THR365 4.1 42.5 1.0 N A:ASP367 4.2 45.5 1.0 CA A:ASP359 4.2 45.0 1.0 CB A:ASP363 4.3 47.4 1.0 CB A:ASP359 4.3 44.5 1.0 CA A:THR365 4.4 41.8 1.0 CG A:GLU370 4.4 45.9 1.0 N A:ASP361 4.4 56.2 1.0 N A:GLY362 4.4 50.7 1.0 CB A:ASP361 4.4 55.6 1.0 CB A:ASP367 4.5 48.1 1.0 N A:ILE366 4.5 43.7 1.0 O A:HOH605 4.5 44.3 1.0 C A:ASP359 4.5 46.0 1.0 N A:GLY364 4.6 45.8 1.0 CA A:ASP363 4.6 45.7 1.0 CA A:ILE366 4.7 44.3 1.0 N A:ALA360 4.7 46.6 1.0 OD1 A:ASP367 4.7 62.2 1.0 CA A:ASP361 4.8 53.8 1.0 C A:ASP361 4.8 52.9 1.0 CB A:THR365 4.9 44.8 1.0 C A:ASP363 4.9 45.8 1.0 C A:ILE366 4.9 44.5 1.0 CA A:ASP367 5.0 46.1 1.0

Calcium binding site 4 out of 4 in the Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of Rsgcamp Double Mutant ILE80HIS/VAL116ILE in the Off State (Illuminated) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca510 b:62.4 occ:1.00 OD1 A:ASN400 2.3 72.2 1.0 OE2 A:GLU407 2.3 54.9 1.0 O A:TYR402 2.3 55.0 1.0 OE1 A:GLU407 2.4 64.8 1.0 OD1 A:ASP398 2.5 82.9 1.0 CD A:GLU407 2.7 63.3 1.0 OD1 A:ASP396 2.7 73.3 1.0 OD2 A:ASP398 2.8 89.8 1.0 CG A:ASP398 2.9 91.0 1.0 CG A:ASN400 3.3 75.1 1.0 C A:TYR402 3.6 60.3 1.0 CG A:ASP396 3.7 70.7 1.0 ND2 A:ASN400 4.0 74.2 1.0 CG A:GLU407 4.2 70.0 1.0 CA A:ASP396 4.2 72.9 1.0 N A:TYR402 4.3 62.1 1.0 CB A:ASP398 4.3 88.0 1.0 CB A:ASP396 4.4 75.6 1.0 CB A:ASN400 4.4 75.5 1.0 N A:ASP398 4.4 91.5 1.0 N A:ILE403 4.5 58.4 1.0 CA A:TYR402 4.5 63.5 1.0 OD2 A:ASP396 4.5 68.2 1.0 N A:ASN400 4.5 66.5 1.0 N A:SER404 4.5 72.8 1.0 CA A:ILE403 4.5 67.8 1.0 C A:ASP396 4.6 77.3 1.0 N A:LYS397 4.6 81.8 1.0 N A:GLY399 4.7 82.8 1.0 CA A:ASP398 4.8 83.5 1.0 CA A:ASN400 4.9 68.0 1.0 N A:GLY401 4.9 62.4 1.0 CB A:TYR402 5.0 69.5 1.0 C A:ILE403 5.0 73.5 1.0

J.P.Fuenzalida-Werner, K.Mishra, R.Janowski, D.Niessing, A.C.Stiel. Genetically Encoded Photo-Switchable Indicators, New Tools For Optoacoustic Imaging and Fluorescence Nanoscopy To Be Published.