Calcium in PDB 6zyj: Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium

The structure of Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium, PDB code: 6zyj was solved by Y.Yan, S.Preissler, D.Ron, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	73.71 / 1.85
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	73.677, 78.039, 75.377, 90.00, 101.26, 90.00
R / Rfree (%)	21.8 / 24.7

The binding sites of Calcium atom in the Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium (pdb code 6zyj). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium, PDB code: 6zyj:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca502 b:26.5 occ:1.00 O A:HOH649 2.2 26.8 1.0 O A:HIS227 2.3 21.8 1.0 O A:HOH792 2.4 29.2 1.0 O A:HOH788 2.4 37.6 1.0 O A:HOH763 2.4 29.1 1.0 OD2 A:ASP232 2.5 20.8 1.0 OD1 A:ASP232 2.7 20.0 1.0 CG A:ASP232 2.9 20.0 1.0 C A:HIS227 3.4 21.6 1.0 CA A:HIS227 4.2 22.2 1.0 O A:HOH794 4.3 41.5 1.0 O A:HOH684 4.3 33.3 1.0 CB A:HIS227 4.3 22.6 1.0 N A:LEU228 4.4 20.9 1.0 CB A:ASP232 4.4 19.8 1.0 CA A:LEU228 4.6 20.4 1.0 O A:HOH663 4.6 36.3 1.0 N A:GLY229 4.8 19.0 1.0 O A:GLY203 4.8 17.4 1.0

Calcium binding site 2 out of 4 in the Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca503 b:18.9 occ:1.00 O A:HOH720 2.2 30.2 1.0 O3B A:ADP504 2.2 16.3 1.0 O1A A:ADP504 2.2 15.5 1.0 O A:HOH733 2.4 19.5 1.0 O A:HOH755 2.4 14.1 1.0 O A:HOH635 2.5 11.4 1.0 O A:HOH780 2.5 21.0 1.0 PB A:ADP504 3.4 16.5 1.0 PA A:ADP504 3.4 15.2 1.0 O3A A:ADP504 3.6 15.7 1.0 O A:HOH689 3.8 18.8 1.0 OD2 A:ASP10 4.1 14.3 1.0 O1B A:ADP504 4.1 16.4 1.0 CA A:GLY338 4.2 17.8 1.0 O A:HOH603 4.2 20.9 1.0 OD1 A:ASP199 4.3 17.7 1.0 O2A A:ADP504 4.4 15.4 1.0 O A:HOH667 4.4 15.8 1.0 O A:HOH743 4.5 11.9 1.0 O5' A:ADP504 4.5 15.3 1.0 CA A:GLY201 4.6 16.2 1.0 O2B A:ADP504 4.6 16.6 1.0 OD1 A:ASP10 4.7 13.8 1.0 CG A:ASP10 4.8 14.2 1.0 N A:GLY338 4.8 18.0 1.0

Calcium binding site 3 out of 4 in the Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca604 b:17.6 occ:1.00 O1B B:ADP602 2.2 15.4 1.0 O B:HOH809 2.2 18.3 1.0 O1A B:ADP602 2.3 16.4 1.0 O B:HOH849 2.3 21.8 1.0 O B:HOH842 2.4 15.2 1.0 O B:HOH808 2.4 18.3 1.0 O B:HOH732 2.5 15.2 1.0 PB B:ADP602 3.4 15.9 1.0 PA B:ADP602 3.4 16.0 1.0 O3A B:ADP602 3.5 16.1 1.0 O B:HOH769 3.9 20.4 1.0 O3B B:ADP602 4.1 15.6 1.0 OD2 B:ASP10 4.1 15.6 1.0 CA B:GLY338 4.2 18.0 1.0 O B:HOH745 4.2 24.5 1.0 OD1 B:ASP199 4.3 18.4 1.0 O B:HOH759 4.4 15.8 1.0 O2A B:ADP602 4.4 16.4 1.0 O5' B:ADP602 4.5 16.7 1.0 CA B:GLY201 4.5 15.9 1.0 O2B B:ADP602 4.6 15.8 1.0 O B:HOH790 4.6 15.1 1.0 OD1 B:ASP10 4.8 15.0 1.0 CG B:ASP10 4.8 14.9 1.0 N B:GLY338 4.9 18.4 1.0

Calcium binding site 4 out of 4 in the Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of HSC70 Atpase Domain in Complex with Adp and Calcium within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca605 b:26.4 occ:1.00 O B:HOH801 2.1 30.9 1.0 O B:HOH866 2.3 28.6 1.0 O B:HIS227 2.3 21.4 1.0 OD2 B:ASP232 2.5 20.2 1.0 OD1 B:ASP232 2.6 20.2 1.0 O B:HOH777 2.6 25.5 1.0 CG B:ASP232 2.9 19.9 1.0 C B:HIS227 3.4 20.9 1.0 CA B:HIS227 4.2 21.3 1.0 CB B:HIS227 4.3 21.4 1.0 N B:LEU228 4.4 20.6 1.0 CB B:ASP232 4.4 19.4 1.0 CA B:LEU228 4.5 20.6 1.0 N B:GLY229 4.8 20.1 1.0 O B:GLY203 4.9 17.6 1.0

S.Preissler, C.Rato, Y.Yan, L.A.Perera, A.Czako, D.Ron. Calcium Depletion Challenges Endoplasmic Reticulum Proteostasis By Destabilising Bip-Substrate Complexes. Elife V. 9 2020.
ISSN: ESSN 2050-084X
PubMed: 33295873
DOI: 10.7554/ELIFE.62601