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Calcium in PDB 7aqp: T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris

Enzymatic activity of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris

All present enzymatic activity of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris:
3.4.17.18;

Protein crystallography data

The structure of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris, PDB code: 7aqp was solved by V.I.Timofeev, V.K.Akparov, I.P.Kuranova, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 29.91 / 2.60
Space group P 63 2 2
Cell size a, b, c (Å), α, β, γ (°) 158.278, 158.278, 104.610, 90.00, 90.00, 120.00
R / Rfree (%) 16.4 / 20.8

Other elements in 7aqp:

The structure of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris also contains other interesting chemical elements:

Zinc (Zn) 1 atom

Calcium Binding Sites:

The binding sites of Calcium atom in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris (pdb code 7aqp). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris, PDB code: 7aqp:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in 7aqp

Go back to Calcium Binding Sites List in 7aqp
Calcium binding site 1 out of 4 in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca402

b:83.8
occ:1.00
 O A:SER7 2.3 51.2 1.0
O A:HOH586 2.5 48.3 1.0
OE1 A:GLU14 2.5 46.7 1.0
O A:TYR9 2.5 40.1 1.0
CD A:GLU14 3.2 42.2 1.0
OE2 A:GLU14 3.4 55.7 1.0
C A:SER7 3.5 43.8 1.0
C A:TYR9 3.6 33.3 1.0
CA A:HIS10 4.2 27.7 1.0
N A:HIS10 4.3 29.1 1.0
C A:GLY8 4.3 30.1 1.0
CA A:SER7 4.4 46.5 1.0
CG A:GLU14 4.4 36.4 1.0
N A:TYR9 4.4 30.9 1.0
N A:GLY8 4.5 36.4 1.0
N A:ASN11 4.6 27.8 1.0
O A:GLY8 4.6 30.8 1.0
CA A:GLY8 4.7 32.0 1.0
CA A:TYR9 4.7 33.2 1.0
CB A:GLU14 4.7 33.6 1.0
CB A:SER7 4.8 44.2 1.0
C A:HIS10 4.8 26.3 1.0

Calcium binding site 2 out of 4 in 7aqp

Go back to Calcium Binding Sites List in 7aqp
Calcium binding site 2 out of 4 in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca403

b:66.9
occ:1.00
 OD1 A:ASP51 2.4 76.5 1.0
O A:ASP51 2.5 45.0 1.0
OE1 A:GLU59 2.6 56.6 1.0
OG A:SER50 2.6 51.5 1.0
O A:HOH523 2.8 31.2 1.0
CG A:ASP51 2.9 64.8 1.0
OE1 A:GLU57 3.0 91.0 1.0
OE2 A:GLU59 3.1 59.0 1.0
CD A:GLU59 3.2 55.5 1.0
OE2 A:GLU57 3.3 63.2 1.0
OD2 A:ASP51 3.3 63.1 1.0
CD A:GLU57 3.3 68.9 1.0
CB A:SER50 3.4 44.5 1.0
C A:ASP51 3.5 42.6 1.0
N A:ASP51 3.9 43.2 1.0
CB A:ASP51 3.9 45.8 1.0
CA A:ASP51 4.0 42.4 1.0
C A:SER50 4.2 43.9 1.0
CB A:GLU57 4.3 60.1 1.0
CA A:SER50 4.3 44.3 1.0
OE2 A:GLU104 4.3 49.5 1.0
CG A:GLU57 4.4 66.3 1.0
CG A:GLU104 4.5 42.4 1.0
O A:ASN52 4.5 41.2 1.0
O A:VAL100 4.5 31.7 1.0
O A:ARG103 4.6 38.1 1.0
N A:ASN52 4.6 46.6 1.0
CG A:GLU59 4.7 53.2 1.0
O A:ASN101 4.7 41.7 1.0
C A:ASN52 4.8 45.8 1.0
O A:SER50 4.8 41.0 1.0
CD A:GLU104 4.9 51.0 1.0

Calcium binding site 3 out of 4 in 7aqp

Go back to Calcium Binding Sites List in 7aqp
Calcium binding site 3 out of 4 in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca404

b:58.1
occ:1.00
 OE1 A:GLU104 2.3 50.7 1.0
OD1 A:ASP56 2.5 61.8 1.0
OE2 A:GLU61 2.6 47.6 1.0
O A:GLU57 2.8 52.0 1.0
O A:GLU59 3.3 40.9 1.0
CD A:GLU104 3.5 51.0 1.0
CD A:GLU61 3.5 44.1 1.0
O A:HOH501 3.6 90.7 1.0
CG A:ASP56 3.7 63.2 1.0
CG A:GLU61 3.7 39.3 1.0
C A:GLU57 4.0 57.9 1.0
O A:HOH520 4.0 48.7 1.0
N A:GLU59 4.1 56.6 1.0
NH2 A:ARG189 4.1 49.1 1.0
OD2 A:ASP56 4.1 67.0 1.0
OE1 A:GLN196 4.2 38.1 1.0
OE2 A:GLU104 4.3 49.5 1.0
C A:GLU59 4.4 43.7 1.0
CG A:GLU104 4.4 42.4 1.0
CG A:GLN196 4.5 38.8 1.0
CB A:GLN196 4.5 36.9 1.0
N A:GLU57 4.6 53.5 1.0
OE1 A:GLU61 4.6 45.4 1.0
CD A:GLN196 4.8 39.2 1.0
CA A:GLU59 4.8 50.7 1.0
C A:ASP56 4.8 53.2 1.0
CA A:ASN58 4.9 66.7 1.0
N A:ASN58 4.9 65.9 1.0
CB A:GLU104 4.9 39.2 1.0
CB A:ASP56 4.9 63.5 1.0
CA A:GLU57 5.0 56.3 1.0
C A:ASN58 5.0 63.2 1.0

Calcium binding site 4 out of 4 in 7aqp

Go back to Calcium Binding Sites List in 7aqp
Calcium binding site 4 out of 4 in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca405

b:92.1
occ:1.00
 O A:TYR5 2.3 51.0 1.0
OE2 A:GLU292 2.7 56.5 1.0
OD1 A:ASP291 2.7 39.7 1.0
OE1 A:GLU292 2.8 48.4 1.0
CD A:GLU292 3.1 52.0 1.0
C A:TYR5 3.3 46.2 1.0
CG A:ASP291 3.7 40.4 1.0
OD1 A:ASP6 3.8 42.9 1.0
CA A:TYR5 4.1 48.3 1.0
CB A:TYR5 4.1 46.1 1.0
OD2 A:ASP291 4.3 37.0 1.0
N A:ASP6 4.3 45.6 1.0
N A:ASP291 4.3 35.3 1.0
CA A:ASP6 4.5 48.9 1.0
CD1 A:TYR5 4.6 51.6 1.0
N A:GLU292 4.6 33.4 1.0
CG A:GLU292 4.6 47.4 1.0
CB A:PRO290 4.7 36.2 1.0
CB A:ASP291 4.8 39.0 1.0
CG A:TYR5 4.9 50.1 1.0
CG A:ASP6 4.9 44.2 1.0
CA A:ASP291 4.9 35.2 1.0
CA A:PRO290 5.0 35.9 1.0

Reference:

V.I.Timofeev, V.K.Akparov, I.P.Kuranova. T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris To Be Published.
Page generated: Thu Jul 18 23:06:11 2024

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