Atomistry » Calcium » PDB 7aq0-7b1g » 7aqp
Atomistry »
  Calcium »
    PDB 7aq0-7b1g »
      7aqp »

Calcium in PDB 7aqp: T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris

Enzymatic activity of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris

All present enzymatic activity of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris:
3.4.17.18;

Protein crystallography data

The structure of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris, PDB code: 7aqp was solved by V.I.Timofeev, V.K.Akparov, I.P.Kuranova, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 29.91 / 2.60
Space group P 63 2 2
Cell size a, b, c (Å), α, β, γ (°) 158.278, 158.278, 104.610, 90.00, 90.00, 120.00
R / Rfree (%) 16.4 / 20.8

Other elements in 7aqp:

The structure of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris also contains other interesting chemical elements:

Zinc (Zn) 1 atom

Calcium Binding Sites:

The binding sites of Calcium atom in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris (pdb code 7aqp). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris, PDB code: 7aqp:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in 7aqp

Go back to Calcium Binding Sites List in 7aqp
Calcium binding site 1 out of 4 in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca402

b:83.8
occ:1.00
 O A:SER7 2.3 51.2 1.0
O A:HOH586 2.5 48.3 1.0
OE1 A:GLU14 2.5 46.7 1.0
O A:TYR9 2.5 40.1 1.0
CD A:GLU14 3.2 42.2 1.0
OE2 A:GLU14 3.4 55.7 1.0
C A:SER7 3.5 43.8 1.0
C A:TYR9 3.6 33.3 1.0
CA A:HIS10 4.2 27.7 1.0
N A:HIS10 4.3 29.1 1.0
C A:GLY8 4.3 30.1 1.0
CA A:SER7 4.4 46.5 1.0
CG A:GLU14 4.4 36.4 1.0
N A:TYR9 4.4 30.9 1.0
N A:GLY8 4.5 36.4 1.0
N A:ASN11 4.6 27.8 1.0
O A:GLY8 4.6 30.8 1.0
CA A:GLY8 4.7 32.0 1.0
CA A:TYR9 4.7 33.2 1.0
CB A:GLU14 4.7 33.6 1.0
CB A:SER7 4.8 44.2 1.0
C A:HIS10 4.8 26.3 1.0

Calcium binding site 2 out of 4 in 7aqp

Go back to Calcium Binding Sites List in 7aqp
Calcium binding site 2 out of 4 in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca403

b:66.9
occ:1.00
 OD1 A:ASP51 2.4 76.5 1.0
O A:ASP51 2.5 45.0 1.0
OE1 A:GLU59 2.6 56.6 1.0
OG A:SER50 2.6 51.5 1.0
O A:HOH523 2.8 31.2 1.0
CG A:ASP51 2.9 64.8 1.0
OE1 A:GLU57 3.0 91.0 1.0
OE2 A:GLU59 3.1 59.0 1.0
CD A:GLU59 3.2 55.5 1.0
OE2 A:GLU57 3.3 63.2 1.0
OD2 A:ASP51 3.3 63.1 1.0
CD A:GLU57 3.3 68.9 1.0
CB A:SER50 3.4 44.5 1.0
C A:ASP51 3.5 42.6 1.0
N A:ASP51 3.9 43.2 1.0
CB A:ASP51 3.9 45.8 1.0
CA A:ASP51 4.0 42.4 1.0
C A:SER50 4.2 43.9 1.0
CB A:GLU57 4.3 60.1 1.0
CA A:SER50 4.3 44.3 1.0
OE2 A:GLU104 4.3 49.5 1.0
CG A:GLU57 4.4 66.3 1.0
CG A:GLU104 4.5 42.4 1.0
O A:ASN52 4.5 41.2 1.0
O A:VAL100 4.5 31.7 1.0
O A:ARG103 4.6 38.1 1.0
N A:ASN52 4.6 46.6 1.0
CG A:GLU59 4.7 53.2 1.0
O A:ASN101 4.7 41.7 1.0
C A:ASN52 4.8 45.8 1.0
O A:SER50 4.8 41.0 1.0
CD A:GLU104 4.9 51.0 1.0

Calcium binding site 3 out of 4 in 7aqp

Go back to Calcium Binding Sites List in 7aqp
Calcium binding site 3 out of 4 in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca404

b:58.1
occ:1.00
 OE1 A:GLU104 2.3 50.7 1.0
OD1 A:ASP56 2.5 61.8 1.0
OE2 A:GLU61 2.6 47.6 1.0
O A:GLU57 2.8 52.0 1.0
O A:GLU59 3.3 40.9 1.0
CD A:GLU104 3.5 51.0 1.0
CD A:GLU61 3.5 44.1 1.0
O A:HOH501 3.6 90.7 1.0
CG A:ASP56 3.7 63.2 1.0
CG A:GLU61 3.7 39.3 1.0
C A:GLU57 4.0 57.9 1.0
O A:HOH520 4.0 48.7 1.0
N A:GLU59 4.1 56.6 1.0
NH2 A:ARG189 4.1 49.1 1.0
OD2 A:ASP56 4.1 67.0 1.0
OE1 A:GLN196 4.2 38.1 1.0
OE2 A:GLU104 4.3 49.5 1.0
C A:GLU59 4.4 43.7 1.0
CG A:GLU104 4.4 42.4 1.0
CG A:GLN196 4.5 38.8 1.0
CB A:GLN196 4.5 36.9 1.0
N A:GLU57 4.6 53.5 1.0
OE1 A:GLU61 4.6 45.4 1.0
CD A:GLN196 4.8 39.2 1.0
CA A:GLU59 4.8 50.7 1.0
C A:ASP56 4.8 53.2 1.0
CA A:ASN58 4.9 66.7 1.0
N A:ASN58 4.9 65.9 1.0
CB A:GLU104 4.9 39.2 1.0
CB A:ASP56 4.9 63.5 1.0
CA A:GLU57 5.0 56.3 1.0
C A:ASN58 5.0 63.2 1.0

Calcium binding site 4 out of 4 in 7aqp

Go back to Calcium Binding Sites List in 7aqp
Calcium binding site 4 out of 4 in the T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca405

b:92.1
occ:1.00
 O A:TYR5 2.3 51.0 1.0
OE2 A:GLU292 2.7 56.5 1.0
OD1 A:ASP291 2.7 39.7 1.0
OE1 A:GLU292 2.8 48.4 1.0
CD A:GLU292 3.1 52.0 1.0
C A:TYR5 3.3 46.2 1.0
CG A:ASP291 3.7 40.4 1.0
OD1 A:ASP6 3.8 42.9 1.0
CA A:TYR5 4.1 48.3 1.0
CB A:TYR5 4.1 46.1 1.0
OD2 A:ASP291 4.3 37.0 1.0
N A:ASP6 4.3 45.6 1.0
N A:ASP291 4.3 35.3 1.0
CA A:ASP6 4.5 48.9 1.0
CD1 A:TYR5 4.6 51.6 1.0
N A:GLU292 4.6 33.4 1.0
CG A:GLU292 4.6 47.4 1.0
CB A:PRO290 4.7 36.2 1.0
CB A:ASP291 4.8 39.0 1.0
CG A:TYR5 4.9 50.1 1.0
CG A:ASP6 4.9 44.2 1.0
CA A:ASP291 4.9 35.2 1.0
CA A:PRO290 5.0 35.9 1.0

Reference:

V.I.Timofeev, V.K.Akparov, I.P.Kuranova. T262S, A251S, L254S, L211Q Mutant of Carboxypeptidase T From Thermoactinomyces Vulgaris To Be Published.
Page generated: Thu Jul 18 23:06:11 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy