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Calcium in PDB 7cef: Crystal Structure of Pet-Degrading Cutinase CUT190 /S226P/R228S/ Mutant with the C-Terminal Three Residues Deletion

Enzymatic activity of Crystal Structure of Pet-Degrading Cutinase CUT190 /S226P/R228S/ Mutant with the C-Terminal Three Residues Deletion

All present enzymatic activity of Crystal Structure of Pet-Degrading Cutinase CUT190 /S226P/R228S/ Mutant with the C-Terminal Three Residues Deletion:
3.1.1.74;

Protein crystallography data

The structure of Crystal Structure of Pet-Degrading Cutinase CUT190 /S226P/R228S/ Mutant with the C-Terminal Three Residues Deletion, PDB code: 7cef was solved by A.Senga, N.Numoto, N.Ito, F.Kawai, M.Oda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 41.72 / 1.60
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 60.512, 49.100, 82.948, 90.00, 107.45, 90.00
R / Rfree (%) 17.7 / 22

Other elements in 7cef:

The structure of Crystal Structure of Pet-Degrading Cutinase CUT190 /S226P/R228S/ Mutant with the C-Terminal Three Residues Deletion also contains other interesting chemical elements:

Zinc (Zn) 17 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of Pet-Degrading Cutinase CUT190 /S226P/R228S/ Mutant with the C-Terminal Three Residues Deletion (pdb code 7cef). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Pet-Degrading Cutinase CUT190 /S226P/R228S/ Mutant with the C-Terminal Three Residues Deletion, PDB code: 7cef:

Calcium binding site 1 out of 1 in 7cef

Go back to Calcium Binding Sites List in 7cef
Calcium binding site 1 out of 1 in the Crystal Structure of Pet-Degrading Cutinase CUT190 /S226P/R228S/ Mutant with the C-Terminal Three Residues Deletion


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Pet-Degrading Cutinase CUT190 /S226P/R228S/ Mutant with the C-Terminal Three Residues Deletion within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca401

b:55.2
occ:1.00
 O A:PHE81 2.4 31.0 1.0
O A:ALA78 2.4 32.3 1.0
O A:HOH672 2.4 39.5 1.0
O A:HOH517 2.4 39.9 1.0
O A:SER76 2.4 33.2 1.0
C A:ALA78 3.5 35.5 1.0
C A:PHE81 3.6 24.2 1.0
C A:SER76 3.6 39.6 1.0
C A:PHE77 3.8 41.0 1.0
N A:ALA78 3.8 30.1 1.0
O A:HOH706 3.9 41.0 1.0
OD1 A:ASN133 3.9 29.9 1.0
CA A:PHE77 4.0 41.0 1.0
N A:PHE81 4.1 28.2 1.0
O A:PHE77 4.2 40.7 1.0
N A:PHE77 4.2 41.9 1.0
CA A:ALA78 4.3 38.6 1.0
N A:GLY80 4.4 30.5 1.0
CA A:GLY82 4.4 27.7 1.0
N A:SER79 4.4 34.0 1.0
N A:GLY82 4.5 27.5 1.0
CA A:PHE81 4.5 28.1 1.0
CA A:SER79 4.5 34.6 1.0
C A:SER79 4.7 35.3 1.0
CA A:SER76 4.7 35.5 1.0

Reference:

A.Senga, N.Numoto, M.Yamashita, A.Iida, N.Ito, F.Kawai, M.Oda. Multiple Structural States of CA2+ Regulated Pet Hydrolase, CUT190, and Its Correlation with Activity and Stability. J.Biochem. 2020.
ISSN: ISSN 0021-924X
PubMed: 32882044
DOI: 10.1093/JB/MVAA102
Page generated: Thu Jul 18 23:40:33 2024

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