Calcium in PDB 7fag: Room Temperature Structure of Elastase with High-Strength Agarose Hydrogel

All present enzymatic activity of Room Temperature Structure of Elastase with High-Strength Agarose Hydrogel:
3.4.21.36;

The structure of Room Temperature Structure of Elastase with High-Strength Agarose Hydrogel, PDB code: 7fag was solved by S.Sugiyama, K.Kakinouchi, H.Adachi, M.Murata, Y.Mori, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.91 / 1.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.209, 58.116, 75.338, 90, 90, 90
R / Rfree (%)	11.5 / 15.3

The binding sites of Calcium atom in the Room Temperature Structure of Elastase with High-Strength Agarose Hydrogel (pdb code 7fag). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Room Temperature Structure of Elastase with High-Strength Agarose Hydrogel, PDB code: 7fag:

Calcium binding site 1 out of 1 in the Room Temperature Structure of Elastase with High-Strength Agarose Hydrogel


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Room Temperature Structure of Elastase with High-Strength Agarose Hydrogel within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca301 b:15.0 occ:0.60 O A:GLN79 2.3 18.7 1.0 O A:ASN76 2.3 15.1 1.0 OE2 A:GLU74 2.3 17.5 1.0 OE2 A:GLU84 2.3 15.8 1.0 OD2 A:ASP81 2.4 25.7 1.0 O A:HOH480 2.4 31.3 1.0 CD A:GLU74 3.3 15.9 1.0 CG A:ASP81 3.3 26.7 1.0 CD A:GLU84 3.4 15.1 1.0 C A:ASN76 3.5 12.8 1.0 C A:GLN79 3.5 19.5 1.0 OE1 A:GLU74 3.6 22.7 1.0 N A:ASP81 3.8 15.7 1.0 CG A:GLU84 3.9 14.8 1.0 N A:ASN76 3.9 12.5 1.0 CB A:ASP81 3.9 24.4 1.0 CA A:ASN76 4.1 13.3 1.0 OD1 A:ASP81 4.2 27.8 1.0 CA A:ASN80 4.3 19.7 1.0 N A:GLN79 4.3 20.0 1.0 N A:ASN80 4.3 20.8 1.0 C A:ASN80 4.3 20.1 1.0 CB A:ASN76 4.4 18.1 1.0 N A:LEU77 4.5 13.1 1.0 CA A:ASP81 4.5 17.1 1.0 OE1 A:GLU84 4.5 14.2 1.0 CA A:GLN79 4.5 23.0 1.0 N A:HIS75 4.5 11.4 1.0 CG A:GLU74 4.6 14.5 1.0 C A:LEU77 4.6 18.7 1.0 CA A:LEU77 4.6 13.9 1.0 O A:LEU77 4.8 21.6 1.0 N A:ASN78 4.9 17.8 1.0 C A:HIS75 5.0 11.4 1.0

S.Sugiyama, K.Kakinouchi, H.Adachi, M.Murata, Y.Mori. Room Temperature Structure of Elastase with High-Strength Agarose Hydrogel To Be Published.