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Calcium in PDB 7kt3: Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN)

Enzymatic activity of Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN)

All present enzymatic activity of Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN):
2.7.7.7;

Protein crystallography data

The structure of Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN), PDB code: 7kt3 was solved by J.A.Jamsen, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 24.54 / 1.88
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 59.804, 68.579, 110.149, 90, 90, 90
R / Rfree (%) 16 / 18.8

Other elements in 7kt3:

The structure of Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN) also contains other interesting chemical elements:

Sodium (Na) 1 atom
Chlorine (Cl) 3 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN) (pdb code 7kt3). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN), PDB code: 7kt3:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in 7kt3

Go back to Calcium Binding Sites List in 7kt3
Calcium binding site 1 out of 3 in the Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca502

b:12.6
occ:1.00
OD2 A:ASP418 2.3 14.1 1.0
OD1 A:ASP332 2.3 10.4 1.0
OD2 A:ASP330 2.3 19.9 1.0
O P:HOH107 2.4 16.1 1.0
O2A A:8DG501 2.5 13.6 1.0
O3' P:DA4 2.6 15.3 1.0
OD1 A:ASP330 2.6 14.1 1.0
CG A:ASP330 2.8 16.1 1.0
CG A:ASP332 3.4 12.0 1.0
CG A:ASP418 3.4 14.1 1.0
PA A:8DG501 3.5 13.4 1.0
C3' P:DA4 3.7 14.8 1.0
OD2 A:ASP332 3.8 12.7 1.0
O5' A:8DG501 3.8 14.0 1.0
CA A:CA503 3.9 12.8 1.0
O1A A:8DG501 3.9 14.1 1.0
C4' P:DA4 4.0 15.1 1.0
C5' P:DA4 4.0 16.4 1.0
CB A:ASP418 4.1 13.4 1.0
C5' A:8DG501 4.3 11.4 1.0
OD1 A:ASP418 4.3 15.8 1.0
CB A:ASP330 4.3 14.4 1.0
CE1 A:HIS329 4.5 34.4 1.0
NH2 A:ARG416 4.6 15.7 1.0
OP1 P:DA4 4.6 15.1 1.0
CB A:ASP332 4.7 10.5 1.0
CZ3 A:TRP434 4.7 16.0 1.0
NE2 A:HIS329 4.7 36.6 1.0
O5' P:DA4 4.8 13.6 1.0
O A:VAL331 4.8 13.0 1.0
O3A A:8DG501 4.9 14.6 1.0
C2' P:DA4 5.0 15.5 1.0

Calcium binding site 2 out of 3 in 7kt3

Go back to Calcium Binding Sites List in 7kt3
Calcium binding site 2 out of 3 in the Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca503

b:12.8
occ:1.00
O1B A:8DG501 2.3 11.1 1.0
OD2 A:ASP332 2.3 12.7 1.0
O1G A:8DG501 2.3 18.1 1.0
OD1 A:ASP330 2.3 14.1 1.0
O2A A:8DG501 2.4 13.6 1.0
O A:HOH762 2.5 14.9 1.0
O A:ASP330 2.7 14.9 1.0
CG A:ASP332 3.3 12.0 1.0
PB A:8DG501 3.5 14.1 1.0
C A:ASP330 3.5 12.4 1.0
CG A:ASP330 3.5 16.1 1.0
PG A:8DG501 3.5 23.9 1.0
PA A:8DG501 3.7 13.4 1.0
OD1 A:ASP332 3.7 10.4 1.0
O3B A:8DG501 3.8 20.6 1.0
CA A:CA502 3.9 12.6 1.0
O3A A:8DG501 3.9 14.6 1.0
N A:ASP330 4.0 15.0 1.0
O A:HOH665 4.1 12.6 1.0
CA A:ASP330 4.1 14.7 1.0
O2G A:8DG501 4.2 26.3 1.0
O A:HOH601 4.3 21.6 1.0
N A:VAL331 4.4 12.6 1.0
CB A:ASP330 4.4 14.4 1.0
OD2 A:ASP330 4.4 19.9 1.0
N A:GLY320 4.4 11.4 1.0
CE1 A:HIS329 4.5 34.4 1.0
ND1 A:HIS329 4.5 40.0 1.0
O3G A:8DG501 4.7 27.0 1.0
CA A:VAL331 4.7 11.8 1.0
CB A:ASP332 4.7 10.5 1.0
O5' A:8DG501 4.7 14.0 1.0
C5' A:8DG501 4.7 11.4 1.0
CA A:GLY319 4.7 12.5 1.0
O1A A:8DG501 4.7 14.1 1.0
C A:VAL331 4.8 12.7 1.0
N A:ASP332 4.8 12.4 1.0
O2B A:8DG501 4.8 18.9 1.0
O P:HOH107 5.0 16.1 1.0

Calcium binding site 3 out of 3 in 7kt3

Go back to Calcium Binding Sites List in 7kt3
Calcium binding site 3 out of 3 in the Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca504

b:49.3
occ:0.69
O D:HOH116 2.3 36.2 1.0
O A:HOH726 2.3 38.3 1.0
O A:PHE205 2.4 31.6 1.0
O D:HOH106 2.4 26.6 0.8
O A:HOH633 2.7 44.8 1.0
C A:PHE205 3.5 22.5 1.0
O A:LEU202 4.3 24.5 1.0
N A:GLY206 4.4 20.9 1.0
CA A:GLY206 4.4 26.9 1.0
CA A:PHE205 4.5 19.8 1.0
CB A:PHE205 4.7 18.5 1.0
OP1 D:DC3 4.7 19.7 0.3
N A:PHE205 4.8 21.9 1.0
OP2 D:DC3 4.8 25.6 0.3
OP1 D:DC3 4.9 19.4 0.7
N A:GLY201 5.0 38.3 1.0

Reference:

J.A.Jamsen, A.Sassa, D.D.Shock, W.A.Beard, S.H.Wilson. Dna Polymerase Mu, 8-Oxodgtp:at Pre-Catalytic Ground State Ternary Complex, 20 Mm CA2+ (120MIN) To Be Published.
Page generated: Fri Jul 19 01:24:17 2024

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