Calcium in PDB 7lj4: Human Traak K+ Channel Fheig Mutant A270P in A K+ Bound Conductive Conformation

The structure of Human Traak K+ Channel Fheig Mutant A270P in A K+ Bound Conductive Conformation, PDB code: 7lj4 was solved by R.A.Rietmeijer, S.G.Brohawn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.66 / 2.78
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	80.28, 136.88, 95.58, 90, 94.24, 90
R / Rfree (%)	21.5 / 25.8

The structure of Human Traak K+ Channel Fheig Mutant A270P in A K+ Bound Conductive Conformation also contains other interesting chemical elements:

Potassium

(K)

7 atoms

The binding sites of Calcium atom in the Human Traak K+ Channel Fheig Mutant A270P in A K+ Bound Conductive Conformation (pdb code 7lj4). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Human Traak K+ Channel Fheig Mutant A270P in A K+ Bound Conductive Conformation, PDB code: 7lj4:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Human Traak K+ Channel Fheig Mutant A270P in A K+ Bound Conductive Conformation


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Human Traak K+ Channel Fheig Mutant A270P in A K+ Bound Conductive Conformation within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca305 b:120.7 occ:1.00 O A:SER112 2.1 85.8 1.0 OD2 A:ASP249 2.2 150.5 1.0 OD1 A:ASP115 2.4 91.6 1.0 OD2 A:ASP115 2.4 92.5 1.0 OG A:SER118 2.5 76.7 1.0 CG A:ASP249 2.5 153.3 1.0 CG A:ASP115 2.6 91.5 1.0 OD1 A:ASP249 2.7 156.8 1.0 C A:SER112 3.3 85.3 1.0 CB A:SER118 3.6 76.0 1.0 CB A:ASP249 3.7 150.3 1.0 CB A:ASP115 4.1 91.3 1.0 N A:SER112 4.1 95.4 1.0 N A:ALA113 4.2 80.7 1.0 CA A:SER112 4.2 89.5 1.0 CA A:ALA113 4.3 79.2 1.0 N A:SER118 4.4 80.9 1.0 CA A:SER118 4.6 77.4 1.0 CA A:ASP249 4.6 154.1 1.0 CB A:SER112 4.6 91.0 1.0 N A:ASP115 4.6 85.4 1.0 C A:HIS111 4.8 101.3 1.0 CA A:ASP115 4.8 88.7 1.0 C A:ALA113 4.8 80.0 1.0 O A:ASP115 5.0 84.2 1.0

Calcium binding site 2 out of 3 in the Human Traak K+ Channel Fheig Mutant A270P in A K+ Bound Conductive Conformation


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Human Traak K+ Channel Fheig Mutant A270P in A K+ Bound Conductive Conformation within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca306 b:118.1 occ:1.00 O A:GLY98 2.4 63.3 1.0 O B:HOH402 3.0 39.9 1.0 OE2 B:GLU58 3.2 58.0 1.0 O A:HIS111 3.4 98.7 1.0 C A:GLY98 3.6 62.0 1.0 CD B:GLU58 3.6 57.5 1.0 CG B:GLU58 3.8 55.5 1.0 CB A:ASP100 3.8 74.4 1.0 N A:ASP100 4.2 66.9 1.0 C A:ALA99 4.2 63.5 1.0 N A:ALA113 4.3 80.7 1.0 CB A:ALA113 4.4 76.9 1.0 OE1 B:GLU58 4.5 59.6 1.0 N A:ALA99 4.5 61.5 1.0 CA A:GLY98 4.5 61.7 1.0 O A:ALA99 4.5 63.0 1.0 CA A:ALA99 4.5 62.6 1.0 CA A:ASP100 4.5 70.1 1.0 C A:HIS111 4.6 101.3 1.0 CG A:ASP100 4.7 76.5 1.0 OE1 B:GLN62 4.9 49.1 1.0 CA A:SER112 4.9 89.5 1.0 OD1 A:ASP100 4.9 77.0 1.0

Calcium binding site 3 out of 3 in the Human Traak K+ Channel Fheig Mutant A270P in A K+ Bound Conductive Conformation


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Human Traak K+ Channel Fheig Mutant A270P in A K+ Bound Conductive Conformation within 5.0Å range: probe atom residue distance (Å) B Occ G:Ca301 b:50.6 occ:1.00 OE1 G:GLU10 2.3 57.6 1.0 OE2 G:GLU10 2.6 60.5 1.0 O G:LYS19 2.7 46.8 1.0 CD G:GLU10 2.7 58.3 1.0 C G:LYS19 3.9 47.3 1.0 N G:LYS19 4.1 50.8 1.0 CG G:GLU10 4.1 56.7 1.0 CA G:LYS19 4.5 49.4 1.0 CB G:LYS19 4.8 52.7 1.0 O G:HOH414 4.8 45.0 1.0 CG G:MET18 4.9 54.0 1.0 N G:THR20 5.0 45.1 1.0

R.A.Rietmeijer, B.Sorum, B.Li, S.G.Brohawn. Physical Basis For Distinct Basal and Mechanically-Gated Activity of the Human K + Channel Traak Biorxiv 2021.
DOI: 10.1101/2021.06.04.447000