Calcium in PDB 7og0: Nontypeable Haemophillus Influenzae Sapa in Open and Closed Conformations, in Complex with Double Stranded Rna

The structure of Nontypeable Haemophillus Influenzae Sapa in Open and Closed Conformations, in Complex with Double Stranded Rna, PDB code: 7og0 was solved by P.Lukacik, C.D.Owen, J.E.Nettleship, L.E.Bird, R.J.Owens, M.A.Walsh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	65.74 / 2.61
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	143.26, 147.97, 59.55, 90, 90, 90
R / Rfree (%)	19.7 / 24.9

The binding sites of Calcium atom in the Nontypeable Haemophillus Influenzae Sapa in Open and Closed Conformations, in Complex with Double Stranded Rna (pdb code 7og0). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Nontypeable Haemophillus Influenzae Sapa in Open and Closed Conformations, in Complex with Double Stranded Rna, PDB code: 7og0:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Nontypeable Haemophillus Influenzae Sapa in Open and Closed Conformations, in Complex with Double Stranded Rna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Nontypeable Haemophillus Influenzae Sapa in Open and Closed Conformations, in Complex with Double Stranded Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca601 b:68.3 occ:1.00 OD1 A:ASN486 2.4 51.0 1.0 OE2 A:GLU449 2.7 73.5 1.0 CG A:ASN486 3.5 52.7 1.0 CD A:GLU449 3.8 77.4 1.0 ND2 A:ASN486 4.0 53.4 1.0 OE1 A:GLU449 4.1 79.2 1.0 CB A:GLU480 4.3 71.5 1.0 CG A:GLN447 4.5 96.5 1.0 CA A:GLU480 4.5 64.7 1.0 CB A:ASN486 4.7 54.3 1.0 O A:GLU480 4.9 64.0 1.0 O A:LEU444 4.9 83.6 1.0 O A:ASN483 5.0 47.7 1.0

Calcium binding site 2 out of 2 in the Nontypeable Haemophillus Influenzae Sapa in Open and Closed Conformations, in Complex with Double Stranded Rna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Nontypeable Haemophillus Influenzae Sapa in Open and Closed Conformations, in Complex with Double Stranded Rna within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca601 b:41.9 occ:1.00 O B:HOH726 2.2 26.7 1.0 O B:HOH762 2.3 32.3 1.0 OD1 B:ASN486 2.4 47.2 1.0 OE2 B:GLU449 2.9 61.4 1.0 OE1 B:GLU449 3.0 64.4 1.0 CD B:GLU449 3.3 65.3 1.0 CG B:ASN486 3.5 46.1 1.0 ND2 B:ASN486 3.9 45.3 1.0 O B:HOH743 4.2 32.7 1.0 O B:GLU480 4.3 57.4 1.0 CB B:GLU480 4.4 61.4 1.0 CD2 B:LEU444 4.6 59.5 1.0 CG B:GLU449 4.6 68.0 1.0 CB B:ASN486 4.7 44.7 1.0 OE1 B:GLU480 4.8 75.0 1.0 CA B:GLU480 4.8 56.3 1.0 O B:LEU444 4.8 70.5 1.0 OD2 B:ASP325 4.9 52.9 1.0 O B:ASN483 4.9 39.9 1.0 CG B:GLN447 5.0 78.0 1.0

P.Lukacik, C.D.Owen, G.Harris, J.R.Bolla, S.Picaud, I.Alibay, J.E.Nettleship, L.E.Bird, R.J.Owens, P.C.Biggin, P.Filippakopoulos, C.V.Robinson, M.A.Walsh. The Structure of Nontypeable Haemophilus Influenzae Sapa in A Closed Conformation Reveals A Constricted Ligand-Binding Cavity and A Novel Rna Binding Motif. Plos One V. 16 56070 2021.
ISSN: ESSN 1932-6203
PubMed: 34653190
DOI: 10.1371/JOURNAL.PONE.0256070