Calcium in PDB 7taa: Family 13 Alpha Amylase in Complex with Acarbose

All present enzymatic activity of Family 13 Alpha Amylase in Complex with Acarbose:
3.2.1.1;

The structure of Family 13 Alpha Amylase in Complex with Acarbose, PDB code: 7taa was solved by G.J.Davies, A.M.Brzozowski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.98
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.040, 67.183, 133.560, 90.00, 90.00, 90.00
R / Rfree (%)	15.9 / 23.6

The binding sites of Calcium atom in the Family 13 Alpha Amylase in Complex with Acarbose (pdb code 7taa). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Family 13 Alpha Amylase in Complex with Acarbose, PDB code: 7taa:

Calcium binding site 1 out of 1 in the Family 13 Alpha Amylase in Complex with Acarbose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Family 13 Alpha Amylase in Complex with Acarbose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca480 b:15.0 occ:1.00 O A:HOH492 2.4 13.7 1.0 O A:HOH507 2.5 15.0 1.0 O A:GLU162 2.5 17.8 1.0 OD1 A:ASN121 2.5 13.0 1.0 OD2 A:ASP175 2.5 13.1 1.0 OD1 A:ASP175 2.5 15.5 1.0 O A:HIS210 2.5 15.3 1.0 O A:HOH505 2.8 19.3 1.0 CG A:ASP175 2.9 20.0 1.0 C A:GLU162 3.6 16.9 1.0 CG A:ASN121 3.7 16.6 1.0 C A:HIS210 3.7 21.0 1.0 CA A:GLU162 4.2 10.0 1.0 ND2 A:ASN121 4.2 11.9 1.0 CB A:HIS210 4.3 15.8 1.0 CB A:ASP175 4.4 12.5 1.0 O A:LEU176 4.4 16.6 1.0 O A:ASN121 4.4 12.8 1.0 ND1 A:HIS147 4.5 21.3 1.0 CG2 A:VAL211 4.5 20.7 1.0 CE1 A:HIS147 4.5 25.0 1.0 O A:CYS164 4.5 17.5 1.0 CA A:HIS210 4.6 20.1 1.0 N A:VAL211 4.6 12.8 1.0 CA A:VAL211 4.7 14.7 1.0 N A:ASP163 4.7 16.1 1.0 O A:VAL161 4.7 17.8 1.0 CB A:ASN121 4.8 13.1 1.0 O A:HOH590 4.9 21.5 1.0 CA A:ASP163 4.9 21.7 1.0

A.M.Brzozowski, G.J.Davies. Structure of the Aspergillus Oryzae Alpha-Amylase Complexed with the Inhibitor Acarbose at 2.0 A Resolution. Biochemistry V. 36 10837 1997.
ISSN: ISSN 0006-2960
PubMed: 9283074
DOI: 10.1021/BI970539I