Calcium in PDB 7xks: Crystal Structure of An Alkaline Pectate Lyase From Bacillus Clausii

All present enzymatic activity of Crystal Structure of An Alkaline Pectate Lyase From Bacillus Clausii:
4.2.2.2;

The structure of Crystal Structure of An Alkaline Pectate Lyase From Bacillus Clausii, PDB code: 7xks was solved by C.Zhou, Y.Y.Zheng, W.D.Liu, Y.Ma, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	27.27 / 1.78
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	109.085, 109.085, 44.977, 90, 90, 90
R / Rfree (%)	15.4 / 18.9

The binding sites of Calcium atom in the Crystal Structure of An Alkaline Pectate Lyase From Bacillus Clausii (pdb code 7xks). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of An Alkaline Pectate Lyase From Bacillus Clausii, PDB code: 7xks:

Calcium binding site 1 out of 1 in the Crystal Structure of An Alkaline Pectate Lyase From Bacillus Clausii


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of An Alkaline Pectate Lyase From Bacillus Clausii within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca407 b:29.3 occ:1.00 O A:HOH601 2.0 26.7 1.0 O A:HOH539 2.0 23.2 1.0 OD2 A:ASP170 2.1 20.2 1.0 OD2 A:ASP166 2.1 22.0 1.0 OD1 A:ASP136 2.2 15.7 1.0 OD2 A:ASP136 2.3 24.4 1.0 CG A:ASP136 2.5 20.2 1.0 CG A:ASP170 3.2 20.3 1.0 CG A:ASP166 3.2 25.4 1.0 CB A:ASP166 3.7 18.2 1.0 CB A:ASP170 3.8 19.3 1.0 N A:GLY167 3.9 18.5 1.0 CB A:ASP136 4.0 18.2 1.0 NZ A:LYS172 4.1 29.4 1.0 OD1 A:ASP170 4.2 20.1 1.0 O A:ASP134 4.3 17.4 1.0 OD1 A:ASP166 4.3 25.6 1.0 O A:HOH576 4.3 23.2 1.0 CE A:LYS172 4.4 35.8 1.0 CA A:GLY167 4.6 17.8 1.0 CA A:ASP136 4.8 17.1 1.0 CA A:ASP166 4.9 18.0 1.0 C A:ASP166 4.9 20.6 1.0 O A:HOH558 4.9 29.8 1.0

C.Zhou, Y.Cao, Y.Xue, W.Liu, J.Ju, Y.Ma. Structure of An Alkaline Pectate Lyase and Rational Engineering with Improved Thermo-Alkaline Stability For Efficient Ramie Degumming. Int J Mol Sci V. 24 2022.
ISSN: ESSN 1422-0067
PubMed: 36613981
DOI: 10.3390/IJMS24010538