Calcium in PDB 8c0t: Nrs 1.2: Fluorescent Sensors For Imaging Interstitial Calcium

The structure of Nrs 1.2: Fluorescent Sensors For Imaging Interstitial Calcium, PDB code: 8c0t was solved by J.Basquin, O.Griesbeck, A.Valiente-Gabioud, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.85 / 1.28
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	54.332, 62.854, 95.709, 90, 90, 90
R / Rfree (%)	18.9 / 20.2

The binding sites of Calcium atom in the Nrs 1.2: Fluorescent Sensors For Imaging Interstitial Calcium (pdb code 8c0t). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Nrs 1.2: Fluorescent Sensors For Imaging Interstitial Calcium, PDB code: 8c0t:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Nrs 1.2: Fluorescent Sensors For Imaging Interstitial Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Nrs 1.2: Fluorescent Sensors For Imaging Interstitial Calcium within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:16.6 occ:1.00 O A:ARG205 2.3 17.7 1.0 OD1 A:ASP199 2.3 16.1 1.0 OD1 A:ASP201 2.3 17.8 1.0 OD1 A:ASN203 2.4 19.0 1.0 OE1 A:GLU210 2.4 16.8 1.0 O A:HOH573 2.5 21.0 1.0 OE2 A:GLU210 2.6 19.2 1.0 CD A:GLU210 2.8 16.5 1.0 CG A:ASP201 3.3 19.3 1.0 CG A:ASN203 3.4 22.0 1.0 H A:ASN203 3.4 20.5 1.0 H A:ARG205 3.5 21.7 1.0 HA A:ASP199 3.5 18.5 1.0 CG A:ASP199 3.5 16.9 1.0 C A:ARG205 3.5 16.6 1.0 H A:ASP201 3.6 21.9 1.0 HD21 A:ASN203 3.6 26.4 1.0 HA A:ILE206 3.7 19.4 1.0 OD2 A:ASP201 3.7 21.9 1.0 H A:ASP207 3.8 19.7 1.0 ND2 A:ASN203 3.9 21.9 1.0 CA A:ASP199 4.2 15.4 1.0 N A:ARG205 4.2 18.1 1.0 N A:ASN203 4.2 17.0 1.0 H A:LYS202 4.2 21.4 1.0 HB2 A:ARG205 4.3 27.7 1.0 CB A:ASP199 4.3 16.9 1.0 CG A:GLU210 4.3 16.4 1.0 OD2 A:ASP199 4.4 16.7 1.0 N A:ASP201 4.4 18.2 1.0 N A:ILE206 4.4 16.1 1.0 HB2 A:ASP199 4.4 20.4 1.0 CA A:ILE206 4.4 16.1 1.0 H A:THR200 4.5 20.9 1.0 CA A:ARG205 4.5 18.0 1.0 C A:ASP199 4.5 18.0 1.0 N A:ASP207 4.5 16.4 1.0 OD2 A:ASP207 4.5 21.8 1.0 H A:GLY204 4.5 20.2 1.0 CB A:ASN203 4.5 19.3 1.0 CB A:ASP201 4.5 19.4 1.0 N A:LYS202 4.6 17.8 1.0 N A:THR200 4.6 17.4 1.0 HD22 A:ASN203 4.7 26.4 1.0 HG3 A:GLU210 4.7 19.8 1.0 HB3 A:ASN203 4.7 23.2 1.0 CA A:ASN203 4.8 17.1 1.0 CA A:ASP201 4.8 18.8 1.0 HG2 A:GLU210 4.8 19.8 1.0 C A:ASP201 4.8 18.7 1.0 N A:GLY204 4.8 16.8 1.0 HB3 A:ASP207 4.8 20.8 1.0 HB3 A:ASP201 4.9 23.3 1.0 CB A:ARG205 4.9 23.1 1.0 HB2 A:GLU210 4.9 19.5 1.0 CG A:ASP207 4.9 19.2 1.0 C A:ASN203 4.9 17.6 1.0 C A:ILE206 5.0 16.6 1.0 O A:HOH533 5.0 29.5 1.0

Calcium binding site 2 out of 2 in the Nrs 1.2: Fluorescent Sensors For Imaging Interstitial Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Nrs 1.2: Fluorescent Sensors For Imaging Interstitial Calcium within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:21.4 occ:1.00 OD1 A:ASP163 2.3 23.9 1.0 O A:PHE169 2.3 21.4 1.0 OD1 A:ASN167 2.4 25.3 1.0 OE1 A:GLU174 2.4 21.0 1.0 OD1 A:ASP165 2.4 29.9 1.0 OE2 A:GLU174 2.5 27.2 1.0 O A:HOH529 2.6 28.5 1.0 CD A:GLU174 2.8 23.6 1.0 H A:ASN167 3.3 32.0 1.0 CG A:ASP165 3.3 31.5 1.0 CG A:ASN167 3.3 27.7 1.0 CG A:ASP163 3.4 22.4 1.0 C A:PHE169 3.5 21.1 1.0 H A:PHE169 3.5 26.0 1.0 HA A:ASP163 3.6 26.5 1.0 OD2 A:ASP165 3.7 36.5 1.0 H A:ASP165 3.7 35.4 1.0 H A:ASP171 3.7 24.5 1.0 HA A:ILE170 3.7 22.0 1.0 HD21 A:ASN167 3.9 38.1 1.0 ND2 A:ASN167 4.0 31.7 1.0 N A:ASN167 4.0 26.6 1.0 H A:GLY166 4.1 37.3 1.0 OD2 A:ASP163 4.2 24.6 1.0 N A:PHE169 4.2 21.7 1.0 HB2 A:PHE169 4.3 27.8 1.0 CA A:ASP163 4.3 22.0 1.0 CG A:GLU174 4.3 23.6 1.0 N A:ASP165 4.3 29.5 1.0 CB A:ASP163 4.3 22.7 1.0 HD13 A:ILE170 4.3 22.8 1.0 CB A:ASN167 4.3 26.8 1.0 HB3 A:ASN167 4.4 32.2 1.0 N A:ASP171 4.4 20.4 1.0 N A:ILE170 4.4 17.1 1.0 CA A:PHE169 4.4 21.0 1.0 CA A:ILE170 4.4 18.3 1.0 C A:ASP163 4.4 23.0 1.0 N A:GLY166 4.5 31.1 1.0 H A:PHE164 4.5 28.8 1.0 CB A:ASP165 4.5 31.2 1.0 H A:GLY168 4.5 28.4 1.0 HB3 A:ASP171 4.6 29.1 1.0 HB2 A:ASP163 4.6 27.3 1.0 OD2 A:ASP171 4.6 31.3 1.0 N A:PHE164 4.6 24.0 1.0 CA A:ASN167 4.6 26.9 1.0 HG3 A:GLU174 4.7 28.3 1.0 HG2 A:GLU174 4.7 28.3 1.0 HB3 A:ASP165 4.7 37.5 1.0 CA A:ASP165 4.7 31.3 1.0 C A:ASP165 4.8 30.0 1.0 HD22 A:ASN167 4.8 38.1 1.0 N A:GLY168 4.8 23.7 1.0 CG A:ASP171 4.9 28.3 1.0 HB2 A:GLU174 4.9 24.8 1.0 C A:ILE170 4.9 17.7 1.0 CB A:PHE169 4.9 23.1 1.0 C A:ASN167 4.9 27.5 1.0 O A:ASP163 4.9 27.9 1.0 O A:HOH676 5.0 32.2 1.0

A.A.Valiente-Gabioud, I.Garteizgogeascoa Suner, A.Idziak, A.Fabritius, J.Basquin, J.Angibaud, U.V.Nagerl, S.P.Singh, O.Griesbeck. Fluorescent Sensors For Imaging of Interstitial Calcium. Nat Commun V. 14 6220 2023.
ISSN: ESSN 2041-1723
PubMed: 37798285
DOI: 10.1038/S41467-023-41928-W