Calcium in PDB 8d8m: Isoreticular, Interpenetrating Co-Crystal of Replication Initiator Protein REPE54 and Symmetrical Expanded Duplex (31MER) Containing the Cognate REPE54 Sequence and An Additional G-C Rich Sequence Grown in A Calcium Chloride Crystallization Solution.

The structure of Isoreticular, Interpenetrating Co-Crystal of Replication Initiator Protein REPE54 and Symmetrical Expanded Duplex (31MER) Containing the Cognate REPE54 Sequence and An Additional G-C Rich Sequence Grown in A Calcium Chloride Crystallization Solution., PDB code: 8d8m was solved by A.R.Orun, C.D.Snow, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.98 / 3.10
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	73.322, 133.189, 134.645, 90, 90, 90
R / Rfree (%)	22.4 / 25.5

The binding sites of Calcium atom in the Isoreticular, Interpenetrating Co-Crystal of Replication Initiator Protein REPE54 and Symmetrical Expanded Duplex (31MER) Containing the Cognate REPE54 Sequence and An Additional G-C Rich Sequence Grown in A Calcium Chloride Crystallization Solution. (pdb code 8d8m). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Isoreticular, Interpenetrating Co-Crystal of Replication Initiator Protein REPE54 and Symmetrical Expanded Duplex (31MER) Containing the Cognate REPE54 Sequence and An Additional G-C Rich Sequence Grown in A Calcium Chloride Crystallization Solution., PDB code: 8d8m:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Isoreticular, Interpenetrating Co-Crystal of Replication Initiator Protein REPE54 and Symmetrical Expanded Duplex (31MER) Containing the Cognate REPE54 Sequence and An Additional G-C Rich Sequence Grown in A Calcium Chloride Crystallization Solution.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Isoreticular, Interpenetrating Co-Crystal of Replication Initiator Protein REPE54 and Symmetrical Expanded Duplex (31MER) Containing the Cognate REPE54 Sequence and An Additional G-C Rich Sequence Grown in A Calcium Chloride Crystallization Solution. within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca101 b:92.6 occ:1.00 OP1 A:DT19 3.0 84.8 1.0 P A:DT19 4.4 84.7 1.0 OP1 B:DG21 4.5 48.1 1.0 O3' A:DA18 4.7 70.1 1.0 C5' A:DT19 4.8 85.1 1.0

Calcium binding site 2 out of 2 in the Isoreticular, Interpenetrating Co-Crystal of Replication Initiator Protein REPE54 and Symmetrical Expanded Duplex (31MER) Containing the Cognate REPE54 Sequence and An Additional G-C Rich Sequence Grown in A Calcium Chloride Crystallization Solution.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Isoreticular, Interpenetrating Co-Crystal of Replication Initiator Protein REPE54 and Symmetrical Expanded Duplex (31MER) Containing the Cognate REPE54 Sequence and An Additional G-C Rich Sequence Grown in A Calcium Chloride Crystallization Solution. within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca301 b:129.2 occ:1.00 O C:HOH401 2.2 97.7 1.0 O C:HOH402 2.2 89.8 1.0 OE2 C:GLU77 2.5 128.3 1.0 OD1 C:ASP81 2.6 96.1 1.0 O A:HOH201 3.4 93.0 1.0 CD C:GLU77 3.7 125.1 1.0 CG C:ASP81 3.8 93.3 1.0 OP2 A:DT20 4.0 88.9 1.0 OD2 C:ASP34 4.2 80.0 1.0 OD2 C:ASP81 4.3 89.2 1.0 CD C:LYS80 4.5 105.4 1.0 OE1 C:GLU77 4.5 119.8 1.0 CG C:GLU77 4.7 123.7 1.0 N7 A:DG21 4.7 103.7 1.0 C7 A:DT20 4.8 89.4 1.0 OP2 A:DG21 4.9 95.9 1.0

A.R.Orun, E.T.Shields, S.Dmytriw, A.Vajapayajula, C.D.Snow. Designed Crystalline Assemblies of Protein and Dna Enable Site-Specific Installation of A Guest Protein, Dna, and Small Molecule. To Be Published.