Calcium in PDB 8ely: Hras R97M Crystal Form 2

All present enzymatic activity of Hras R97M Crystal Form 2:
3.6.5.2;

The structure of Hras R97M Crystal Form 2, PDB code: 8ely was solved by C.W.Johnson, C.Mattos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.98 / 1.75
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	39.241, 39.241, 157.693, 90, 90, 120
R / Rfree (%)	17.9 / 22.7

The structure of Hras R97M Crystal Form 2 also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Calcium atom in the Hras R97M Crystal Form 2 (pdb code 8ely). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Hras R97M Crystal Form 2, PDB code: 8ely:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Hras R97M Crystal Form 2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Hras R97M Crystal Form 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca204 b:31.1 occ:1.00 OD1 A:ASP105 2.3 28.4 1.0 O A:ARG102 2.4 26.2 1.0 O A:HOH422 2.4 34.8 1.0 O A:HOH367 2.5 35.0 1.0 O A:HOH428 2.8 40.9 1.0 CG A:ASP105 3.4 28.9 1.0 C A:ARG102 3.5 29.2 1.0 OD2 A:ASP105 3.8 34.4 1.0 CA A:ARG102 4.3 25.3 1.0 N A:VAL103 4.5 21.1 1.0 CA A:VAL103 4.6 25.8 0.4 CA A:VAL103 4.6 25.8 0.6 CB A:ASP105 4.6 26.3 1.0 O A:VAL103 4.7 26.3 1.0 N A:ASP105 4.7 21.4 1.0 C A:VAL103 4.8 24.6 1.0 CB A:ARG102 4.8 27.1 1.0 CA A:ASP105 4.8 22.6 1.0

Calcium binding site 2 out of 2 in the Hras R97M Crystal Form 2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Hras R97M Crystal Form 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca205 b:49.9 occ:1.00 O A:HOH370 2.4 38.6 0.5 OE2 A:GLU162 2.4 27.2 1.0 O A:HOH437 2.5 25.1 0.5 O A:HOH398 2.5 37.8 1.0 OE1 A:GLU162 2.5 20.8 1.0 O A:HOH410 2.5 31.9 1.0 CD A:GLU162 2.8 25.3 1.0 CG A:GLU162 4.3 19.6 1.0 O A:HOH418 4.3 41.3 1.0 O A:HOH423 4.4 31.8 0.5 O A:HOH432 4.4 38.0 1.0 CG A:PRO140 4.6 24.8 1.0 CD A:PRO140 4.6 21.0 1.0 O A:GLY138 4.7 24.1 1.0 O A:HOH327 4.9 18.9 1.0

C.W.Johnson, S.K.Fetics, K.P.Davis, J.A.Rodrigues, C.Mattos. Allosteric Site Variants Affect Gtp Hydrolysis on Ras. Protein Sci. E4767 2023.
ISSN: ESSN 1469-896X
PubMed: 37615343
DOI: 10.1002/PRO.4767