Calcium in PDB 8h0j: Annexin A5 Mutant

The structure of Annexin A5 Mutant, PDB code: 8h0j was solved by Z.C.Hua, W.Tang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.63 / 2.23
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	69.294, 134.47, 79.227, 90, 90, 90
R / Rfree (%)	18.8 / 24.7

The binding sites of Calcium atom in the Annexin A5 Mutant (pdb code 8h0j). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 5 binding sites of Calcium where determined in the Annexin A5 Mutant, PDB code: 8h0j:
Jump to Calcium binding site number: 1; 2; 3; 4; 5;

Calcium binding site 1 out of 5 in the Annexin A5 Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Annexin A5 Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:81.6 occ:1.00 O A:GLY30 2.4 54.4 1.0 O A:MET28 2.5 40.5 1.0 OE2 A:GLU72 2.5 68.5 1.0 O A:GLY32 2.5 37.5 1.0 OE1 A:GLU72 2.6 44.9 1.0 CD A:GLU72 2.9 45.8 1.0 C A:GLY30 3.5 47.4 1.0 C A:MET28 3.6 54.0 1.0 C A:GLY32 3.6 37.1 1.0 OG1 A:THR33 3.7 36.6 1.0 C A:LEU31 3.8 37.2 1.0 O A:LEU31 3.9 45.9 1.0 N A:GLY32 4.0 35.0 1.0 CA A:MET28 4.2 42.6 1.0 N A:GLY30 4.2 46.3 1.0 CA A:LEU31 4.3 44.2 1.0 CG A:GLU72 4.3 60.4 1.0 N A:LEU31 4.3 44.6 1.0 O A:HOH651 4.3 43.1 1.0 CA A:GLY32 4.4 37.3 1.0 CA A:GLY30 4.5 48.0 1.0 C A:LYS29 4.5 48.6 1.0 N A:THR33 4.6 31.9 1.0 N A:LYS29 4.6 49.0 1.0 CA A:THR33 4.7 43.4 1.0 CB A:MET28 4.8 34.7 1.0 CB A:THR33 4.8 32.8 1.0 O A:LYS29 4.9 45.4 1.0 CA A:LYS29 4.9 59.6 1.0

Calcium binding site 2 out of 5 in the Annexin A5 Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Annexin A5 Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:84.2 occ:1.00 C A:GLY32 3.6 37.1 1.0 O A:GLY32 3.7 37.5 1.0 N A:THR33 3.7 31.9 1.0 O A:ALA27 3.9 38.7 1.0 O A:LYS26 4.0 38.3 1.0 CA A:THR33 4.0 43.4 1.0 CA A:GLY32 4.1 37.3 1.0 N A:ASP34 4.1 38.0 1.0 C A:THR33 4.1 36.0 1.0 O A:LYS29 4.2 45.4 1.0 C A:ALA27 4.3 31.8 1.0 CB A:ASP34 4.7 33.6 1.0 N A:GLY32 4.7 35.0 1.0 CA A:ALA27 4.7 34.0 1.0 O A:THR33 4.7 36.5 1.0 N A:MET28 4.9 35.4 1.0 CA A:ASP34 4.9 48.6 1.0

Calcium binding site 3 out of 5 in the Annexin A5 Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Annexin A5 Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca403 b:62.0 occ:1.00 O A:LYS70 2.5 35.7 1.0 O A:LEU73 2.8 33.0 1.0 OE1 A:GLU78 2.8 34.9 1.0 CD A:GLU78 3.5 32.9 1.0 C A:LYS70 3.7 38.9 1.0 OE2 A:GLU78 3.9 33.4 1.0 C A:LEU73 3.9 34.7 1.0 CG2 A:THR74 4.0 37.9 1.0 CA A:THR74 4.2 41.7 1.0 CA A:SER71 4.3 36.2 1.0 N A:SER71 4.5 32.4 1.0 N A:THR74 4.5 35.7 1.0 O A:SER71 4.5 37.7 1.0 CG A:GLU78 4.6 30.9 1.0 C A:SER71 4.6 33.1 1.0 CB A:THR74 4.7 33.8 1.0 CA A:LYS70 4.7 27.7 1.0

Calcium binding site 4 out of 5 in the Annexin A5 Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Annexin A5 Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca404 b:71.7 occ:1.00 O A:HOH506 3.0 30.7 1.0 O A:HOH555 3.0 36.8 1.0 O A:GLU121 3.1 35.8 1.0 CB A:ALA124 3.6 34.8 1.0 CA A:GLU121 3.7 47.2 1.0 C A:GLU121 3.8 47.2 1.0 CG1 A:ILE125 3.8 32.2 1.0 CB A:GLU121 3.9 43.9 1.0 CG A:GLU121 4.0 38.6 1.0 N A:ILE125 4.2 36.7 1.0 O A:HOH635 4.3 43.7 1.0 CD1 A:ILE125 4.3 30.6 1.0 O A:HOH606 4.4 35.7 1.0 C A:ALA124 4.6 39.6 1.0 CA A:ALA124 4.6 44.0 1.0 CA A:ILE125 4.8 45.6 1.0 CB A:ILE125 4.9 34.3 1.0 N A:GLU121 5.0 46.9 1.0

Calcium binding site 5 out of 5 in the Annexin A5 Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Annexin A5 Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca405 b:88.5 occ:1.00 O A:ALA258 3.3 40.4 1.0 O A:LYS260 3.3 51.4 1.0 O A:GLY263 3.4 49.3 1.0 O A:TYR257 3.5 33.5 1.0 C A:ALA258 3.7 34.4 1.0 O A:ALA262 3.8 72.5 1.0 N A:ASP265 4.0 32.7 1.0 C A:GLY263 4.0 54.9 1.0 N A:MET259 4.2 38.1 1.0 CA A:THR264 4.3 38.9 1.0 CA A:ALA258 4.3 37.6 1.0 C A:MET259 4.4 43.9 1.0 C A:LYS260 4.4 59.8 1.0 CA A:MET259 4.4 49.3 1.0 N A:THR264 4.5 49.1 1.0 C A:THR264 4.5 40.7 1.0 N A:LYS260 4.5 48.2 1.0 C A:TYR257 4.5 41.8 1.0 CB A:ASP265 4.6 34.6 1.0 C A:ALA262 4.7 75.9 1.0 O A:MET259 4.8 61.6 1.0 CA A:ASP265 4.9 32.2 1.0 N A:ALA258 4.9 30.3 1.0

Z.C.Hua, W.Tang. Structure Dissection of the Membrane Aggregation Mechanism Induced By Annexin A5 Mutation To Be Published.