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Calcium in PDB 8ibl: Mes Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability- Improving Mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A InactivationEnzymatic activity of Mes Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability- Improving Mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A Inactivation
All present enzymatic activity of Mes Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability- Improving Mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A Inactivation:
3.1.1.74; Protein crystallography data
The structure of Mes Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability- Improving Mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A Inactivation, PDB code: 8ibl
was solved by
M.Emori,
N.Numoto,
N.Kamiya,
M.Oda,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Other elements in 8ibl:
The structure of Mes Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability- Improving Mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A Inactivation also contains other interesting chemical elements:
Calcium Binding Sites:
The binding sites of Calcium atom in the Mes Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability- Improving Mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A Inactivation
(pdb code 8ibl). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Mes Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability- Improving Mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A Inactivation, PDB code: 8ibl: Jump to Calcium binding site number: 1; 2; Calcium binding site 1 out of 2 in 8iblGo back to Calcium Binding Sites List in 8ibl
Calcium binding site 1 out
of 2 in the Mes Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability- Improving Mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A Inactivation
Mono view Stereo pair view
Calcium binding site 2 out of 2 in 8iblGo back to Calcium Binding Sites List in 8ibl
Calcium binding site 2 out
of 2 in the Mes Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability- Improving Mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A Inactivation
Mono view Stereo pair view
Reference:
N.Numoto,
N.Kamiya,
M.Oda.
Improvement of Thermostability and Activity of Pet-Degrading Enzyme CUT190 Towards A Detailed Understanding and Application of the Enzymatic Reaction Mechanism. Biorxiv 2023.
Page generated: Tue Apr 4 19:48:52 2023
ISSN: ISSN 2692-8205 DOI: 10.1101/2023.02.26.529345 |
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