Atomistry » Calcium » PDB 8hfm-8if8 » 8ibm
Atomistry »
  Calcium »
    PDB 8hfm-8if8 »
      8ibm »

Calcium in PDB 8ibm: Sulfate Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability-Improving Mutations of S226P/R228S/Q138A/D250C- E296C/Q123H/N202H and S176A Inactivation

Enzymatic activity of Sulfate Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability-Improving Mutations of S226P/R228S/Q138A/D250C- E296C/Q123H/N202H and S176A Inactivation

All present enzymatic activity of Sulfate Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability-Improving Mutations of S226P/R228S/Q138A/D250C- E296C/Q123H/N202H and S176A Inactivation:
3.1.1.74;

Protein crystallography data

The structure of Sulfate Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability-Improving Mutations of S226P/R228S/Q138A/D250C- E296C/Q123H/N202H and S176A Inactivation, PDB code: 8ibm was solved by M.Emori, N.Numoto, N.Kamiya, M.Oda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 36.27 / 2.20
Space group P 3
Cell size a, b, c (Å), α, β, γ (°) 83.755, 83.755, 64.752, 90, 90, 120
R / Rfree (%) 23.4 / 26.7

Calcium Binding Sites:

The binding sites of Calcium atom in the Sulfate Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability-Improving Mutations of S226P/R228S/Q138A/D250C- E296C/Q123H/N202H and S176A Inactivation (pdb code 8ibm). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Sulfate Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability-Improving Mutations of S226P/R228S/Q138A/D250C- E296C/Q123H/N202H and S176A Inactivation, PDB code: 8ibm:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 8ibm

Go back to Calcium Binding Sites List in 8ibm
Calcium binding site 1 out of 2 in the Sulfate Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability-Improving Mutations of S226P/R228S/Q138A/D250C- E296C/Q123H/N202H and S176A Inactivation


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Sulfate Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability-Improving Mutations of S226P/R228S/Q138A/D250C- E296C/Q123H/N202H and S176A Inactivation within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca401

b:63.5
occ:1.00
 O A:ALA78 2.3 49.0 1.0
O A:PHE81 2.4 48.8 1.0
O A:SER76 2.4 47.9 1.0
C A:ALA78 3.5 49.8 1.0
C A:PHE81 3.5 39.5 1.0
C A:SER76 3.5 44.6 1.0
C A:PHE77 3.8 47.0 1.0
N A:ALA78 3.8 42.8 1.0
CA A:PHE77 3.9 44.0 1.0
CA A:GLY82 4.2 37.2 1.0
N A:PHE77 4.2 42.1 1.0
O A:PHE77 4.2 45.8 1.0
OD1 A:ASN133 4.3 43.0 1.0
CA A:ALA78 4.3 46.5 1.0
N A:PHE81 4.3 42.2 1.0
N A:GLY82 4.3 43.8 1.0
N A:SER79 4.5 49.9 1.0
CA A:PHE81 4.5 42.1 1.0
CA A:SER76 4.6 42.5 1.0
CA A:SER79 4.7 51.0 1.0
N A:GLY80 4.7 42.4 1.0
C A:GLY82 4.9 40.6 1.0
C A:SER79 4.9 43.5 1.0
CB A:ALA78 5.0 41.5 1.0

Calcium binding site 2 out of 2 in 8ibm

Go back to Calcium Binding Sites List in 8ibm
Calcium binding site 2 out of 2 in the Sulfate Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability-Improving Mutations of S226P/R228S/Q138A/D250C- E296C/Q123H/N202H and S176A Inactivation


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Sulfate Bound Form of Pet-Degrading Cutinase CUT190 with Thermostability-Improving Mutations of S226P/R228S/Q138A/D250C- E296C/Q123H/N202H and S176A Inactivation within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca401

b:60.1
occ:1.00
 O B:ALA78 2.3 51.9 1.0
O B:SER76 2.4 49.9 1.0
O B:PHE81 2.5 47.7 1.0
C B:ALA78 3.5 50.0 1.0
C B:SER76 3.6 44.9 1.0
C B:PHE81 3.6 40.4 1.0
C B:PHE77 3.8 48.1 1.0
N B:ALA78 3.8 45.2 1.0
OD1 B:ASN133 3.9 45.4 1.0
CA B:PHE77 4.0 45.3 1.0
N B:PHE81 4.2 43.5 1.0
N B:PHE77 4.2 42.2 1.0
O B:PHE77 4.2 45.5 1.0
CA B:ALA78 4.3 45.4 1.0
CA B:GLY82 4.3 37.8 1.0
N B:GLY82 4.4 44.2 1.0
N B:SER79 4.4 48.4 1.0
CA B:PHE81 4.5 43.3 1.0
CA B:SER79 4.6 48.7 1.0
N B:GLY80 4.6 47.3 1.0
CA B:SER76 4.7 41.4 1.0
O B:HOH526 4.7 48.6 1.0
C B:SER79 4.8 44.0 1.0

Reference:

N.Numoto, N.Kamiya, M.Oda. Improvement of Thermostability and Activity of Pet-Degrading Enzyme CUT190 Towards A Detailed Understanding and Application of the Enzymatic Reaction Mechanism. Biorxiv 2023.
ISSN: ISSN 2692-8205
DOI: 10.1101/2023.02.26.529345
Page generated: Fri Jul 19 09:38:07 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy