Calcium in PDB 8ol9: Anti-Fixa Fab in Complex with Human Des-(Gla-EGF1) Fixa

All present enzymatic activity of Anti-Fixa Fab in Complex with Human Des-(Gla-EGF1) Fixa:
3.4.21.22;

The structure of Anti-Fixa Fab in Complex with Human Des-(Gla-EGF1) Fixa, PDB code: 8ol9 was solved by E.Johansson, L.A.Svensson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.98 / 2.60
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	61.44, 97.27, 366.47, 90, 90, 90
R / Rfree (%)	26.7 / 32.3

The binding sites of Calcium atom in the Anti-Fixa Fab in Complex with Human Des-(Gla-EGF1) Fixa (pdb code 8ol9). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Anti-Fixa Fab in Complex with Human Des-(Gla-EGF1) Fixa, PDB code: 8ol9:

Calcium binding site 1 out of 1 in the Anti-Fixa Fab in Complex with Human Des-(Gla-EGF1) Fixa


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Anti-Fixa Fab in Complex with Human Des-(Gla-EGF1) Fixa within 5.0Å range: probe atom residue distance (Å) B Occ H:Ca302 b:133.9 occ:1.00 OE2 H:GLU109 2.2 85.5 1.0 O H:GLU114 2.3 100.3 1.0 O H:ASN111 2.3 97.5 1.0 OE2 H:GLU116 2.6 98.2 1.0 OE1 H:GLU116 2.6 101.9 1.0 CD H:GLU116 2.9 98.4 1.0 OE2 H:GLU119 3.0 97.2 1.0 CD H:GLU109 3.3 82.9 1.0 C H:GLU114 3.4 97.0 1.0 C H:ASN111 3.5 92.9 1.0 CD H:GLU119 3.8 91.0 1.0 O H:ILE112 3.9 103.1 1.0 CG H:GLU109 4.0 79.8 1.0 CG H:GLU119 4.1 76.3 1.0 OE1 H:GLU109 4.2 74.8 1.0 N H:ASN111 4.2 85.2 1.0 C H:ILE112 4.2 96.8 1.0 N H:THR115 4.2 92.8 1.0 CA H:THR115 4.3 87.1 1.0 CG H:GLU116 4.3 92.3 1.0 N H:GLU114 4.3 105.0 1.0 CA H:ASN111 4.4 87.0 1.0 CA H:GLU114 4.4 100.7 1.0 N H:GLU116 4.4 89.6 1.0 N H:ILE112 4.4 84.7 1.0 CA H:ILE112 4.4 85.8 1.0 N H:HIS110 4.6 76.7 1.0 OE1 H:GLU119 4.8 91.0 1.0 CB H:ASN111 4.8 82.4 1.0 C H:THR115 4.8 90.8 1.0 CB H:GLU114 4.9 101.7 1.0 CA H:GLU109 5.0 77.0 1.0

G.Per Jr., L.Yi, R.Zhou, J.Zhou, E.Johansson, T.Dong, H.Liu, L.B.Johnsen, S.Lund, L.A.Svensson, H.Zhu, N.Thomas, Z.Yang, H.Ostergaard. Computational Design of N-Linked Glycans For High Throughput Epitope Profiling. Protein Sci. E4726 2023.
ISSN: ESSN 1469-896X
PubMed: 37421602
DOI: 10.1002/PRO.4726