Calcium in PDB 8osi: Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State

The structure of Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State, PDB code: 8osi was solved by L.A.Varfolomeeva, K.M.Boyko, A.Y.Nikolaeva, O.M.Subach, F.V.Subach, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.95 / 2.42
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	151.4, 151.4, 61.969, 90, 90, 120
R / Rfree (%)	19.6 / 23.1

The binding sites of Calcium atom in the Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State (pdb code 8osi). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State, PDB code: 8osi:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca502 b:69.9 occ:1.00 OD1 A:ASP246 2.4 73.4 1.0 OD1 A:ASP242 2.4 69.6 1.0 OD1 A:ASP244 2.4 86.6 1.0 OE2 A:GLU253 2.4 69.2 1.0 O A:PHE248 2.4 93.6 1.0 OE1 A:GLU253 2.6 69.5 1.0 CD A:GLU253 2.8 66.3 1.0 CG A:ASP246 3.0 74.2 1.0 OD2 A:ASP246 3.1 69.2 1.0 CG A:ASP244 3.3 67.9 1.0 C A:PHE248 3.4 68.5 1.0 CG A:ASP242 3.5 68.4 1.0 OD2 A:ASP244 3.6 69.4 1.0 N A:PHE248 4.2 65.8 1.0 CA A:PHE248 4.3 63.6 1.0 N A:ILE249 4.3 74.3 1.0 OD2 A:ASP242 4.3 62.9 1.0 CA A:ASP242 4.3 60.1 1.0 CG A:GLU253 4.4 69.3 1.0 CB A:ASP242 4.4 65.4 1.0 CB A:ASP246 4.4 60.6 1.0 N A:ASP244 4.4 57.6 1.0 CA A:ILE249 4.4 73.6 1.0 N A:ASP246 4.4 69.5 1.0 N A:ASN245 4.6 62.0 1.0 C A:ASP242 4.6 54.8 1.0 CB A:PHE248 4.7 68.8 1.0 N A:ARG243 4.7 62.2 1.0 CB A:ASP244 4.7 59.0 1.0 CG2 A:VAL250 4.8 68.3 1.0 CA A:ASP244 4.9 70.8 1.0 CA A:ASP246 4.9 73.3 1.0 N A:VAL250 4.9 66.8 1.0 C A:ASP244 4.9 68.3 1.0

Calcium binding site 2 out of 4 in the Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca503 b:98.5 occ:1.00 OE2 A:GLU289 2.4 112.3 1.0 OD1 A:ASP282 2.4 128.9 1.0 OD1 A:ASP278 2.4 102.9 1.0 O A:ARG284 2.4 110.3 1.0 OD1 A:ASP280 2.5 97.3 1.0 OE1 A:GLU289 2.7 109.9 1.0 CD A:GLU289 2.9 103.0 1.0 CG A:ASP282 3.1 131.4 1.0 C A:ARG284 3.5 117.5 1.0 OD2 A:ASP282 3.5 113.9 1.0 CG A:ASP280 3.6 103.1 1.0 CG A:ASP278 3.6 109.2 1.0 OD2 A:ASP280 4.2 89.2 1.0 CB A:ASP282 4.3 119.2 1.0 N A:ARG284 4.3 124.9 1.0 CA A:ARG284 4.3 124.6 1.0 CG A:GLU289 4.4 92.4 1.0 OD2 A:ASP278 4.4 127.9 1.0 OD2 A:ASP286 4.4 96.3 1.0 N A:ILE285 4.5 81.3 1.0 CB A:ARG284 4.5 147.1 1.0 CB A:ASP278 4.6 99.8 1.0 N A:ASP286 4.6 65.3 1.0 N A:ASP282 4.6 139.6 1.0 CA A:ILE285 4.6 67.4 1.0 CB A:ASP280 4.7 102.8 1.0 N A:GLN279 4.8 80.6 1.0 CA A:ASP278 4.8 103.2 1.0 CA A:ASP282 4.9 126.4 1.0 N A:ASP280 4.9 99.9 1.0 C A:ASP280 5.0 136.5 1.0 N A:GLY283 5.0 102.2 1.0 N A:GLY281 5.0 133.0 1.0

Calcium binding site 3 out of 4 in the Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca504 b:70.6 occ:1.00 OD1 A:ASP169 2.4 61.8 1.0 O A:GLN175 2.4 61.9 1.0 O A:HOH639 2.4 73.4 1.0 OD1 A:ASP171 2.4 77.0 1.0 OD1 A:ASP173 2.4 87.8 1.0 OE1 A:GLU180 2.5 66.0 1.0 CD A:GLU180 3.0 67.9 1.0 OE2 A:GLU180 3.1 66.3 1.0 CG A:ASP171 3.2 65.8 1.0 CG A:ASP173 3.2 81.5 1.0 OD2 A:ASP171 3.3 81.0 1.0 CG A:ASP169 3.6 75.8 1.0 C A:GLN175 3.6 65.1 1.0 OD2 A:ASP173 3.9 79.4 1.0 O A:ILE176 4.0 63.3 1.0 CB A:ASP173 4.1 61.2 1.0 N A:ASP173 4.2 72.0 1.0 N A:GLN175 4.3 68.8 1.0 OD2 A:ASP169 4.3 81.6 1.0 CA A:GLN175 4.4 72.5 1.0 CB A:GLN175 4.4 64.5 1.0 CG A:GLU180 4.4 77.8 1.0 N A:ASP171 4.5 60.7 1.0 CB A:ASP171 4.5 55.2 1.0 CB A:ASP169 4.5 72.5 1.0 CA A:ASP169 4.6 64.8 1.0 CA A:ASP173 4.6 69.1 1.0 N A:ILE176 4.6 63.8 1.0 C A:ILE176 4.7 60.3 1.0 C A:ASP169 4.7 84.7 1.0 CA A:ILE176 4.7 53.1 1.0 N A:LEU170 4.8 71.6 1.0 N A:GLY172 4.8 79.4 1.0 OG1 A:THR177 4.8 59.4 1.0 CA A:ASP171 4.9 81.8 1.0 C A:ASP173 5.0 74.8 1.0

Calcium binding site 4 out of 4 in the Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Genetically Encoded Green Ratiometric Calcium Indicator Fncamp in Calcium-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca505 b:74.2 occ:1.00 OD1 A:ASP205 2.3 70.3 1.0 OE2 A:GLU216 2.4 67.5 1.0 OD1 A:ASP209 2.4 84.0 1.0 O A:THR211 2.5 81.8 1.0 OD1 A:ASP213 2.5 99.0 1.0 OE1 A:GLU216 2.6 64.6 1.0 CD A:GLU216 2.8 62.6 1.0 CG A:ASP209 3.1 89.7 1.0 OD2 A:ASP209 3.2 81.9 1.0 C A:THR211 3.5 58.7 1.0 CG A:ASP205 3.6 74.7 1.0 CG A:ASP213 3.8 95.4 1.0 CA A:GLY207 4.2 88.4 1.0 N A:ILE212 4.2 56.2 1.0 OD2 A:ASP213 4.4 72.6 1.0 CG A:GLU216 4.4 50.4 1.0 CB A:ASP205 4.4 63.1 1.0 N A:ASP213 4.4 55.9 1.0 N A:GLY207 4.4 76.8 1.0 OG1 A:THR211 4.5 150.3 1.0 CA A:ILE212 4.5 55.0 1.0 OD2 A:ASP205 4.5 63.1 1.0 CB A:ASP209 4.5 78.5 1.0 CA A:THR211 4.5 72.6 1.0 OE1 A:GLN175 4.5 83.4 1.0 N A:THR211 4.5 81.1 1.0 CA A:ASP205 4.6 77.8 1.0 N A:GLY208 4.7 90.0 1.0 N A:ASP209 4.7 66.9 1.0 C A:GLY207 4.7 101.6 1.0 C A:ILE212 4.8 62.9 1.0 C A:ASP205 4.9 88.8 1.0 CB A:ASP213 4.9 74.9 1.0

O.M.Subach, L.Varfolomeeva, A.V.Vlaskina, Y.K.Agapova, A.Y.Nikolaeva, K.D.Piatkevich, M.V.Patrushev, K.M.Boyko, F.V.Subach. Fncamp, Ratiometric Green Calcium Indicator Based on Mneongreen Protein. Biochem.Biophys.Res.Commun. V. 665 169 2023.
ISSN: ESSN 1090-2104
PubMed: 37163937
DOI: 10.1016/J.BBRC.2023.04.108