Calcium in PDB 8owm: Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate
Enzymatic activity of Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate
All present enzymatic activity of Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate:
1.4.1.3;
Protein crystallography data
The structure of Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate, PDB code: 8owm
was solved by
M.Grzechowiak,
M.Ruszkowski,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
65.70 /
1.70
|
Space group
|
P 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
95.544,
95.629,
95.841,
90.42,
93.59,
117.78
|
R / Rfree (%)
|
14.4 /
17.2
|
Other elements in 8owm:
The structure of Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate also contains other interesting chemical elements:
Calcium Binding Sites:
The binding sites of Calcium atom in the Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate
(pdb code 8owm). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 6 binding sites of Calcium where determined in the
Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate, PDB code: 8owm:
Jump to Calcium binding site number:
1;
2;
3;
4;
5;
6;
Calcium binding site 1 out
of 6 in 8owm
Go back to
Calcium Binding Sites List in 8owm
Calcium binding site 1 out
of 6 in the Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca510
b:17.4
occ:1.00
|
O
|
A:ILE30
|
2.2
|
19.0
|
1.0
|
OE1
|
F:GLU38
|
2.3
|
24.0
|
1.0
|
O
|
A:HOH686
|
2.3
|
22.2
|
1.0
|
O
|
A:SER27
|
2.3
|
22.7
|
1.0
|
O
|
F:HOH797
|
2.4
|
24.0
|
1.0
|
O
|
A:HOH693
|
2.4
|
18.9
|
1.0
|
CD
|
F:GLU38
|
3.4
|
22.1
|
1.0
|
C
|
A:SER27
|
3.5
|
21.6
|
1.0
|
C
|
A:ILE30
|
3.5
|
19.6
|
1.0
|
OG
|
A:SER27
|
3.9
|
20.4
|
1.0
|
OE2
|
F:GLU38
|
4.0
|
23.4
|
1.0
|
CA
|
A:LEU28
|
4.2
|
19.6
|
1.0
|
O
|
A:HIS58
|
4.2
|
18.9
|
1.0
|
CA
|
A:ILE30
|
4.2
|
18.4
|
1.0
|
N
|
A:ILE30
|
4.3
|
19.5
|
1.0
|
N
|
A:LEU28
|
4.3
|
20.9
|
1.0
|
N
|
A:PHE32
|
4.3
|
17.5
|
1.0
|
CB
|
A:ILE30
|
4.3
|
19.4
|
1.0
|
C
|
A:LEU28
|
4.3
|
19.4
|
1.0
|
C
|
A:PRO31
|
4.3
|
19.6
|
1.0
|
OD2
|
A:ASP59
|
4.4
|
24.8
|
1.0
|
CA
|
A:SER27
|
4.4
|
19.3
|
1.0
|
O
|
A:LEU28
|
4.4
|
21.6
|
1.0
|
N
|
A:PRO31
|
4.4
|
22.2
|
1.0
|
CA
|
A:PRO31
|
4.5
|
21.3
|
1.0
|
O
|
A:HOH627
|
4.6
|
21.8
|
1.0
|
O
|
A:HOH833
|
4.6
|
25.5
|
1.0
|
CG
|
F:GLU38
|
4.6
|
22.4
|
1.0
|
CB
|
F:GLU38
|
4.7
|
20.7
|
1.0
|
O
|
A:PRO31
|
4.8
|
19.3
|
1.0
|
CB
|
A:SER27
|
4.8
|
20.3
|
1.0
|
O
|
F:HOH705
|
4.8
|
25.5
|
1.0
|
CA
|
A:PHE32
|
4.9
|
19.6
|
1.0
|
C
|
A:MET29
|
5.0
|
23.5
|
0.5
|
CG2
|
A:ILE30
|
5.0
|
21.8
|
1.0
|
C
|
A:MET29
|
5.0
|
23.5
|
0.5
|
N
|
A:MET29
|
5.0
|
19.9
|
0.5
|
|
Calcium binding site 2 out
of 6 in 8owm
Go back to
Calcium Binding Sites List in 8owm
Calcium binding site 2 out
of 6 in the Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca515
b:22.2
occ:1.00
|
OE1
|
E:GLU38
|
2.3
|
25.2
|
1.0
|
O
|
B:SER27
|
2.3
|
23.8
|
1.0
|
O
|
B:ILE30
|
2.3
|
23.4
|
1.0
|
O
|
E:HOH4524
|
2.4
|
26.9
|
1.0
|
O
|
B:HOH725
|
2.4
|
23.5
|
1.0
|
O
|
B:HOH643
|
2.4
|
25.0
|
1.0
|
C
|
B:SER27
|
3.4
|
24.9
|
1.0
|
CD
|
E:GLU38
|
3.4
|
28.4
|
1.0
|
C
|
B:ILE30
|
3.5
|
23.9
|
1.0
|
OG
|
B:SER27
|
4.0
|
24.2
|
1.0
|
OE2
|
E:GLU38
|
4.0
|
25.1
|
1.0
|
CA
|
B:LEU28
|
4.2
|
22.9
|
1.0
|
N
|
B:LEU28
|
4.2
|
27.0
|
1.0
|
O
|
B:HIS58
|
4.3
|
20.4
|
1.0
|
O
|
B:LEU28
|
4.3
|
23.6
|
1.0
|
C
|
B:LEU28
|
4.3
|
25.4
|
1.0
|
CA
|
B:ILE30
|
4.3
|
22.9
|
1.0
|
N
|
B:ILE30
|
4.3
|
25.0
|
1.0
|
N
|
B:PHE32
|
4.3
|
18.8
|
1.0
|
CA
|
B:SER27
|
4.4
|
25.5
|
1.0
|
CB
|
B:ILE30
|
4.4
|
22.9
|
1.0
|
OD2
|
B:ASP59
|
4.4
|
26.8
|
1.0
|
C
|
B:PRO31
|
4.4
|
22.6
|
1.0
|
N
|
B:PRO31
|
4.5
|
27.2
|
1.0
|
O
|
B:HOH785
|
4.6
|
24.2
|
1.0
|
O
|
B:HOH644
|
4.6
|
26.3
|
1.0
|
CG
|
E:GLU38
|
4.6
|
22.1
|
1.0
|
CA
|
B:PRO31
|
4.6
|
23.1
|
1.0
|
CB
|
E:GLU38
|
4.8
|
21.3
|
1.0
|
O
|
B:PRO31
|
4.8
|
23.1
|
1.0
|
CB
|
B:SER27
|
4.8
|
24.7
|
1.0
|
O
|
E:HOH4447
|
4.9
|
28.8
|
1.0
|
CA
|
B:PHE32
|
4.9
|
20.4
|
1.0
|
C
|
B:MET29
|
5.0
|
25.1
|
1.0
|
CG2
|
B:ILE30
|
5.0
|
24.1
|
1.0
|
N
|
B:MET29
|
5.0
|
26.8
|
1.0
|
|
Calcium binding site 3 out
of 6 in 8owm
Go back to
Calcium Binding Sites List in 8owm
Calcium binding site 3 out
of 6 in the Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Ca513
b:20.4
occ:1.00
|
OE1
|
D:GLU38
|
2.3
|
23.0
|
1.0
|
O
|
D:HOH779
|
2.3
|
26.2
|
1.0
|
O
|
C:ILE30
|
2.3
|
23.1
|
1.0
|
O
|
C:SER27
|
2.3
|
21.7
|
1.0
|
O
|
C:HOH638
|
2.3
|
25.7
|
1.0
|
O
|
C:HOH700
|
2.4
|
21.8
|
1.0
|
C
|
C:SER27
|
3.4
|
21.2
|
1.0
|
CD
|
D:GLU38
|
3.5
|
24.3
|
1.0
|
C
|
C:ILE30
|
3.5
|
25.5
|
1.0
|
OG
|
C:SER27
|
3.9
|
23.2
|
1.0
|
OE2
|
D:GLU38
|
4.0
|
22.7
|
1.0
|
CA
|
C:LEU28
|
4.2
|
23.5
|
1.0
|
N
|
C:LEU28
|
4.2
|
20.2
|
1.0
|
N
|
C:ILE30
|
4.3
|
21.4
|
1.0
|
CA
|
C:ILE30
|
4.3
|
20.0
|
1.0
|
O
|
C:HIS58
|
4.3
|
17.9
|
1.0
|
N
|
C:PHE32
|
4.3
|
20.8
|
1.0
|
OD2
|
C:ASP59
|
4.3
|
26.3
|
1.0
|
C
|
C:LEU28
|
4.3
|
23.6
|
1.0
|
C
|
C:PRO31
|
4.3
|
22.1
|
1.0
|
CA
|
C:SER27
|
4.4
|
21.2
|
1.0
|
O
|
C:LEU28
|
4.4
|
22.6
|
1.0
|
CB
|
C:ILE30
|
4.4
|
21.7
|
1.0
|
N
|
C:PRO31
|
4.5
|
23.8
|
1.0
|
O
|
C:HOH628
|
4.6
|
23.4
|
1.0
|
CA
|
C:PRO31
|
4.6
|
22.3
|
1.0
|
O
|
C:HOH836
|
4.6
|
25.4
|
1.0
|
CG
|
D:GLU38
|
4.6
|
21.9
|
1.0
|
CB
|
D:GLU38
|
4.7
|
21.5
|
1.0
|
O
|
C:PRO31
|
4.7
|
21.4
|
1.0
|
O
|
D:HOH716
|
4.8
|
27.0
|
1.0
|
CB
|
C:SER27
|
4.8
|
22.0
|
1.0
|
CA
|
C:PHE32
|
4.9
|
19.6
|
1.0
|
C
|
C:MET29
|
4.9
|
24.7
|
0.4
|
C
|
C:MET29
|
4.9
|
24.8
|
0.6
|
N
|
C:MET29
|
5.0
|
21.2
|
0.6
|
N
|
C:MET29
|
5.0
|
21.2
|
0.4
|
|
Calcium binding site 4 out
of 6 in 8owm
Go back to
Calcium Binding Sites List in 8owm
Calcium binding site 4 out
of 6 in the Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Ca507
b:21.5
occ:1.00
|
OE1
|
C:GLU38
|
2.2
|
26.1
|
1.0
|
O
|
D:ILE30
|
2.3
|
22.8
|
1.0
|
O
|
D:SER27
|
2.3
|
24.6
|
1.0
|
O
|
D:HOH610
|
2.4
|
26.1
|
1.0
|
O
|
C:HOH837
|
2.4
|
24.7
|
1.0
|
O
|
D:HOH651
|
2.4
|
22.0
|
1.0
|
CD
|
C:GLU38
|
3.4
|
23.4
|
1.0
|
C
|
D:SER27
|
3.4
|
24.0
|
1.0
|
C
|
D:ILE30
|
3.5
|
26.6
|
1.0
|
OG
|
D:SER27
|
3.9
|
24.3
|
1.0
|
OE2
|
C:GLU38
|
3.9
|
24.5
|
1.0
|
CA
|
D:LEU28
|
4.2
|
25.6
|
1.0
|
CA
|
D:ILE30
|
4.3
|
24.5
|
1.0
|
O
|
D:HIS58
|
4.3
|
21.7
|
1.0
|
N
|
D:PHE32
|
4.3
|
20.9
|
1.0
|
N
|
D:LEU28
|
4.3
|
26.6
|
1.0
|
N
|
D:ILE30
|
4.3
|
25.8
|
1.0
|
OD2
|
D:ASP59
|
4.3
|
27.3
|
1.0
|
C
|
D:PRO31
|
4.3
|
21.4
|
1.0
|
C
|
D:LEU28
|
4.3
|
24.8
|
1.0
|
CA
|
D:SER27
|
4.4
|
24.6
|
1.0
|
CB
|
D:ILE30
|
4.4
|
24.7
|
1.0
|
O
|
D:LEU28
|
4.4
|
26.6
|
1.0
|
N
|
D:PRO31
|
4.5
|
25.9
|
1.0
|
CA
|
D:PRO31
|
4.6
|
24.9
|
1.0
|
CG
|
C:GLU38
|
4.6
|
23.6
|
1.0
|
O
|
D:HOH782
|
4.6
|
24.8
|
1.0
|
O
|
D:HOH636
|
4.6
|
23.4
|
1.0
|
CB
|
C:GLU38
|
4.7
|
21.6
|
1.0
|
O
|
D:PRO31
|
4.7
|
23.6
|
1.0
|
CB
|
D:SER27
|
4.7
|
25.8
|
1.0
|
O
|
C:HOH766
|
4.7
|
27.0
|
1.0
|
CA
|
D:PHE32
|
4.9
|
21.3
|
1.0
|
C
|
D:MET29
|
5.0
|
29.4
|
0.6
|
C
|
D:MET29
|
5.0
|
29.3
|
0.4
|
CG2
|
D:ILE30
|
5.0
|
26.6
|
1.0
|
|
Calcium binding site 5 out
of 6 in 8owm
Go back to
Calcium Binding Sites List in 8owm
Calcium binding site 5 out
of 6 in the Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 5 of Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Ca4211
b:19.9
occ:1.00
|
O
|
E:ILE30
|
2.2
|
22.3
|
1.0
|
OE1
|
B:GLU38
|
2.2
|
22.9
|
1.0
|
O
|
E:HOH4377
|
2.3
|
23.4
|
1.0
|
O
|
E:HOH4356
|
2.3
|
20.9
|
1.0
|
O
|
E:SER27
|
2.3
|
23.6
|
1.0
|
O
|
B:HOH806
|
2.4
|
24.4
|
1.0
|
C
|
E:SER27
|
3.4
|
23.0
|
1.0
|
CD
|
B:GLU38
|
3.4
|
26.2
|
1.0
|
C
|
E:ILE30
|
3.5
|
22.1
|
1.0
|
OG
|
E:SER27
|
3.9
|
23.7
|
1.0
|
OE2
|
B:GLU38
|
4.0
|
24.6
|
1.0
|
CA
|
E:LEU28
|
4.2
|
24.4
|
1.0
|
N
|
E:ILE30
|
4.2
|
21.6
|
1.0
|
CA
|
E:ILE30
|
4.2
|
22.1
|
1.0
|
N
|
E:LEU28
|
4.2
|
24.3
|
1.0
|
O
|
E:HIS58
|
4.2
|
20.6
|
1.0
|
N
|
E:PHE32
|
4.3
|
21.0
|
1.0
|
C
|
E:PRO31
|
4.3
|
20.1
|
1.0
|
OD2
|
E:ASP59
|
4.4
|
27.9
|
1.0
|
CB
|
E:ILE30
|
4.4
|
21.6
|
1.0
|
C
|
E:LEU28
|
4.4
|
24.1
|
1.0
|
CA
|
E:SER27
|
4.4
|
22.9
|
1.0
|
O
|
E:LEU28
|
4.4
|
25.1
|
1.0
|
N
|
E:PRO31
|
4.5
|
23.7
|
1.0
|
CA
|
E:PRO31
|
4.5
|
23.6
|
1.0
|
O
|
E:HOH4515
|
4.6
|
26.5
|
1.0
|
O
|
E:HOH4317
|
4.6
|
22.5
|
1.0
|
CG
|
B:GLU38
|
4.6
|
22.9
|
1.0
|
O
|
E:PRO31
|
4.7
|
20.3
|
1.0
|
CB
|
B:GLU38
|
4.7
|
21.5
|
1.0
|
CB
|
E:SER27
|
4.8
|
22.3
|
1.0
|
O
|
B:HOH759
|
4.8
|
25.6
|
1.0
|
CA
|
E:PHE32
|
4.9
|
21.1
|
1.0
|
C
|
E:MET29
|
4.9
|
26.5
|
1.0
|
|
Calcium binding site 6 out
of 6 in 8owm
Go back to
Calcium Binding Sites List in 8owm
Calcium binding site 6 out
of 6 in the Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 6 of Crystal Structure of Glutamate Dehydrogenase 2 From Arabidopsis Thaliana Binding Ca, Nad and 2,2-Dihydroxyglutarate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Ca507
b:19.6
occ:1.00
|
O
|
F:SER27
|
2.2
|
23.0
|
1.0
|
O
|
A:HOH806
|
2.3
|
26.5
|
1.0
|
O
|
F:ILE30
|
2.3
|
23.7
|
1.0
|
OE1
|
A:GLU38
|
2.3
|
25.5
|
1.0
|
O
|
F:HOH667
|
2.3
|
22.3
|
1.0
|
O
|
F:HOH620
|
2.3
|
23.5
|
1.0
|
C
|
F:SER27
|
3.4
|
20.8
|
1.0
|
CD
|
A:GLU38
|
3.5
|
22.7
|
1.0
|
C
|
F:ILE30
|
3.5
|
25.4
|
1.0
|
OG
|
F:SER27
|
3.9
|
22.9
|
1.0
|
OE2
|
A:GLU38
|
4.0
|
23.8
|
1.0
|
CA
|
F:LEU28
|
4.2
|
21.2
|
1.0
|
N
|
F:LEU28
|
4.2
|
22.9
|
1.0
|
O
|
F:HIS58
|
4.2
|
21.2
|
1.0
|
CA
|
F:ILE30
|
4.2
|
21.9
|
1.0
|
N
|
F:ILE30
|
4.2
|
23.4
|
1.0
|
O
|
F:LEU28
|
4.3
|
24.9
|
1.0
|
C
|
F:LEU28
|
4.3
|
25.0
|
1.0
|
OD2
|
F:ASP59
|
4.3
|
26.4
|
1.0
|
CA
|
F:SER27
|
4.3
|
21.7
|
1.0
|
N
|
F:PHE32
|
4.4
|
19.9
|
1.0
|
C
|
F:PRO31
|
4.4
|
18.6
|
1.0
|
CB
|
F:ILE30
|
4.4
|
19.9
|
1.0
|
N
|
F:PRO31
|
4.5
|
21.2
|
1.0
|
CA
|
F:PRO31
|
4.6
|
19.8
|
1.0
|
O
|
F:HOH634
|
4.6
|
21.0
|
1.0
|
O
|
F:HOH770
|
4.6
|
23.6
|
1.0
|
CG
|
A:GLU38
|
4.7
|
22.1
|
1.0
|
O3
|
A:GOL509
|
4.8
|
26.2
|
1.0
|
CB
|
F:SER27
|
4.8
|
21.8
|
1.0
|
CB
|
A:GLU38
|
4.8
|
22.6
|
1.0
|
O
|
F:PRO31
|
4.8
|
20.4
|
1.0
|
C
|
F:MET29
|
4.9
|
24.0
|
1.0
|
CA
|
F:PHE32
|
4.9
|
21.3
|
1.0
|
|
Reference:
M.Grzechowiak,
J.Sliwiak,
M.Jaskolski,
M.Ruszkowski.
Structural and Functional Studies of Arabidopsis Thaliana Glutamate Dehydrogenase Isoform 2 Demonstrate Enzyme Dynamics and Identify Its Calcium Binding Site. Plant Physiol Biochem. V. 201 07895 2023.
ISSN: ESSN 1873-2690
PubMed: 37478728
DOI: 10.1016/J.PLAPHY.2023.107895
Page generated: Fri Jul 19 11:04:04 2024
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