Calcium in PDB 8p9u: Crystal Structure of Two-Domain Laccase Mutant M199A/D268N From Streptomyces Griseoflavus

All present enzymatic activity of Crystal Structure of Two-Domain Laccase Mutant M199A/D268N From Streptomyces Griseoflavus:
1.10.3.2;

The structure of Crystal Structure of Two-Domain Laccase Mutant M199A/D268N From Streptomyces Griseoflavus, PDB code: 8p9u was solved by I.A.Kolyadenko, S.V.Tishchenko, A.G.Gabdulkhakov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.54 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	74.302, 94.026, 118.927, 90, 91.1, 90
R / Rfree (%)	20.1 / 24.2

The structure of Crystal Structure of Two-Domain Laccase Mutant M199A/D268N From Streptomyces Griseoflavus also contains other interesting chemical elements:

Copper

(Cu)

20 atoms

The binding sites of Calcium atom in the Crystal Structure of Two-Domain Laccase Mutant M199A/D268N From Streptomyces Griseoflavus (pdb code 8p9u). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Two-Domain Laccase Mutant M199A/D268N From Streptomyces Griseoflavus, PDB code: 8p9u:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Two-Domain Laccase Mutant M199A/D268N From Streptomyces Griseoflavus


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Two-Domain Laccase Mutant M199A/D268N From Streptomyces Griseoflavus within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca405 b:45.3 occ:1.00 O C:HOH550 2.5 20.4 1.0 O A:HOH649 2.5 30.6 1.0 O C:HOH586 2.6 18.9 1.0 O A:HOH608 2.6 22.6 1.0 O B:HOH527 2.6 14.9 1.0 O B:HOH524 2.6 21.7 1.0 O B:HOH593 3.5 17.0 1.0 O C:HOH573 3.8 18.0 1.0 O A:HOH531 4.0 16.4 1.0 OG B:SER256 4.2 22.1 1.0 O A:ASP243 4.3 16.6 1.0 O B:ASP243 4.3 17.2 1.0 O A:HOH668 4.3 33.7 1.0 O C:ASP243 4.4 17.8 1.0 OG A:SER256 4.7 12.9 1.0 OG C:SER256 4.8 22.8 1.0 CB B:SER256 4.8 13.7 1.0 CA B:SER256 4.9 20.8 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Two-Domain Laccase Mutant M199A/D268N From Streptomyces Griseoflavus


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Two-Domain Laccase Mutant M199A/D268N From Streptomyces Griseoflavus within 5.0Å range: probe atom residue distance (Å) B Occ F:Ca406 b:44.5 occ:1.00 O E:HOH573 2.4 20.0 1.0 O D:HOH531 2.6 21.8 1.0 O F:HOH548 2.6 14.7 1.0 O D:HOH562 2.7 21.4 1.0 O F:HOH582 2.7 25.8 1.0 O E:HOH558 2.7 27.1 1.0 O D:HOH567 3.7 18.4 1.0 O E:HOH624 4.0 34.7 1.0 O F:HOH552 4.0 22.3 1.0 O E:HOH551 4.0 24.5 1.0 OG E:SER256 4.3 21.4 1.0 O E:ASP243 4.4 21.5 1.0 O D:ASP243 4.4 22.4 1.0 OG F:SER256 4.4 23.0 1.0 O F:ASP243 4.4 20.6 1.0 OG D:SER256 4.7 31.1 1.0 O D:HOH547 4.8 29.0 1.0 CA F:SER256 5.0 20.3 1.0

I.Kolyadenko, S.Tishchenko, A.Gabdulkhakov. Structural Insight Into the Amino Acid Environment of the Two-Domain Laccase'S Trinuclear Copper Cluster. Int J Mol Sci V. 24 2023.
ISSN: ESSN 1422-0067
PubMed: 37569288
DOI: 10.3390/IJMS241511909