Calcium in PDB 8thp: Porx Phosphatase Null Mutant (T271V)

The structure of Porx Phosphatase Null Mutant (T271V), PDB code: 8thp was solved by A.Saran, N.Zeytuni, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.95 / 2.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	86.089, 96.978, 130.94, 90, 90, 90
R / Rfree (%)	25 / 30.5

The binding sites of Calcium atom in the Porx Phosphatase Null Mutant (T271V) (pdb code 8thp). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Porx Phosphatase Null Mutant (T271V), PDB code: 8thp:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Porx Phosphatase Null Mutant (T271V)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Porx Phosphatase Null Mutant (T271V) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca601 b:44.4 occ:1.00 OD1 A:ASP11 2.1 34.6 1.0 OD2 A:ASP54 2.4 50.2 1.0 O A:ASN56 2.4 42.5 1.0 O A:HOH722 2.8 34.1 1.0 CG A:ASP11 2.8 39.5 1.0 OD2 A:ASP11 2.9 42.6 1.0 CG A:ASP54 3.5 40.9 1.0 C A:ASN56 3.7 48.1 1.0 OD1 A:ASP54 4.0 41.8 1.0 OD1 A:ASP10 4.1 60.8 1.0 CB A:ASP11 4.3 40.8 1.0 CD A:GLU12 4.4 53.9 1.0 OE2 A:GLU12 4.5 55.4 1.0 CB A:ASN56 4.5 54.6 1.0 N A:MET57 4.6 49.0 1.0 CA A:ASN56 4.6 47.6 1.0 CA A:MET57 4.6 46.1 1.0 CG A:MET57 4.6 50.6 1.0 OE1 A:GLU12 4.6 48.1 1.0 CB A:ASP54 4.7 41.7 1.0 CG A:GLU12 4.7 54.9 1.0 N A:ASP11 4.7 46.9 1.0 CG A:ASP10 4.8 56.9 1.0 N A:ASN56 4.9 47.7 1.0 N A:GLU12 4.9 53.0 1.0 NZ A:LYS104 4.9 50.4 1.0 OD2 A:ASP10 5.0 62.2 1.0

Calcium binding site 2 out of 4 in the Porx Phosphatase Null Mutant (T271V)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Porx Phosphatase Null Mutant (T271V) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca602 b:52.2 occ:1.00 NE2 A:HIS499 1.9 60.5 1.0 OD1 A:ASP360 2.0 24.7 1.0 NE2 A:HIS364 2.7 56.2 1.0 CG A:ASP360 2.7 26.2 1.0 CE1 A:HIS499 2.9 60.5 1.0 OD2 A:ASP360 2.9 23.3 1.0 CD2 A:HIS499 3.0 57.5 1.0 O A:HOH721 3.1 16.4 1.0 CD2 A:HIS364 3.3 55.6 1.0 CE1 A:HIS364 3.9 61.9 1.0 ND1 A:HIS499 4.0 51.8 1.0 CG A:HIS499 4.1 51.5 1.0 CB A:ASP360 4.2 32.5 1.0 ND2 A:ASN238 4.5 48.5 1.0 CG A:HIS364 4.6 56.7 1.0 OD2 A:ASP237 4.8 40.7 1.0 ND1 A:HIS364 4.9 60.3 1.0

Calcium binding site 3 out of 4 in the Porx Phosphatase Null Mutant (T271V)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Porx Phosphatase Null Mutant (T271V) within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca601 b:48.9 occ:1.00 O B:ASN56 2.3 49.4 1.0 OD1 B:ASP11 2.3 40.1 1.0 O B:HOH704 2.5 27.6 1.0 OD2 B:ASP54 2.6 31.3 1.0 CG B:ASP11 3.0 43.3 1.0 OD2 B:ASP11 3.1 50.4 1.0 C B:ASN56 3.5 46.7 1.0 CG B:ASP54 3.6 34.5 1.0 OE1 B:GLU12 3.9 59.8 1.0 OD1 B:ASP54 4.1 39.1 1.0 CB B:ASN56 4.2 49.9 1.0 CD B:GLU12 4.3 59.9 1.0 CA B:ASN56 4.4 46.3 1.0 OD1 B:ASP10 4.4 44.0 1.0 N B:MET57 4.5 45.0 1.0 CB B:ASP11 4.5 38.8 1.0 CA B:MET57 4.6 52.3 1.0 CG B:GLU12 4.7 51.5 1.0 N B:ASN56 4.8 43.4 1.0 CB B:ASP54 4.8 37.0 1.0 CG B:MET57 4.8 51.2 1.0 OE2 B:GLU12 4.9 59.2 1.0 N B:ASP11 5.0 40.5 1.0

Calcium binding site 4 out of 4 in the Porx Phosphatase Null Mutant (T271V)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Porx Phosphatase Null Mutant (T271V) within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca602 b:31.3 occ:1.00 OD2 B:ASP360 1.8 39.3 1.0 NE2 B:HIS499 2.0 33.4 1.0 NE2 B:HIS364 2.3 31.4 1.0 O B:HOH722 2.5 23.9 1.0 CG B:ASP360 2.7 42.2 1.0 OD1 B:ASP360 2.9 38.3 1.0 CE1 B:HIS499 2.9 32.2 1.0 CD2 B:HIS499 3.0 32.7 1.0 CD2 B:HIS364 3.1 30.2 1.0 CE1 B:HIS364 3.4 34.5 1.0 ND1 B:HIS499 4.0 35.0 1.0 CB B:ASP360 4.1 39.7 1.0 CG B:HIS499 4.1 32.9 1.0 ND2 B:ASN238 4.3 42.9 1.0 CG B:HIS364 4.3 30.9 1.0 ND1 B:HIS364 4.4 32.3 1.0 O B:HOH715 4.6 8.6 1.0 CE1 B:HIS415 4.6 39.2 1.0 OD1 B:ASP237 4.8 46.6 1.0 O B:ASP360 4.8 35.4 1.0 CA B:ASP360 4.9 36.5 1.0 NE2 B:HIS415 4.9 41.3 1.0

A.Saran, N.Zeytuni. Porx Phosphatase Null Mutant (T271V) To Be Published.