Calcium in PDB 8x8g: Crystal Structure of Endosz Mutant D234M, From Streptococcus Equi Subsp. Zooepidemicus SZ105, in Complex with Oligosaccharide G2S2- Oxazoline

The structure of Crystal Structure of Endosz Mutant D234M, From Streptococcus Equi Subsp. Zooepidemicus SZ105, in Complex with Oligosaccharide G2S2- Oxazoline, PDB code: 8x8g was solved by H.H.Guan, C.C.Lin, Y.C.Hsieh, C.J.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.44 / 2.27
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	101.061, 232.603, 50.654, 90, 90, 90
R / Rfree (%)	20.8 / 24.9

The binding sites of Calcium atom in the Crystal Structure of Endosz Mutant D234M, From Streptococcus Equi Subsp. Zooepidemicus SZ105, in Complex with Oligosaccharide G2S2- Oxazoline (pdb code 8x8g). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Endosz Mutant D234M, From Streptococcus Equi Subsp. Zooepidemicus SZ105, in Complex with Oligosaccharide G2S2- Oxazoline, PDB code: 8x8g:

Calcium binding site 1 out of 1 in the Crystal Structure of Endosz Mutant D234M, From Streptococcus Equi Subsp. Zooepidemicus SZ105, in Complex with Oligosaccharide G2S2- Oxazoline


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Endosz Mutant D234M, From Streptococcus Equi Subsp. Zooepidemicus SZ105, in Complex with Oligosaccharide G2S2- Oxazoline within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1002 b:82.0 occ:1.00 O A:PRO900 2.4 57.2 1.0 O A:LEU787 2.5 81.0 1.0 O A:LYS782 2.6 56.7 1.0 OE2 A:GLU901 2.6 74.3 1.0 OD1 A:ASP785 2.7 69.9 1.0 CB A:ASN790 2.8 83.0 1.0 ND2 A:ASN790 2.8 74.6 1.0 CG A:ASN790 3.2 79.5 1.0 CD A:GLU901 3.4 73.5 1.0 C A:PRO900 3.5 54.5 1.0 CG A:ASP785 3.6 71.2 1.0 CG A:GLU901 3.6 74.2 1.0 C A:LEU787 3.6 82.2 1.0 OD2 A:ASP785 3.8 73.9 1.0 N A:LEU787 3.8 80.7 1.0 C A:LYS782 3.8 63.8 1.0 CB A:LEU787 4.0 82.1 1.0 CA A:LEU787 4.0 80.7 1.0 CA A:ASN790 4.2 92.7 1.0 N A:GLU901 4.4 50.0 1.0 CA A:PRO900 4.4 54.0 1.0 OD1 A:ASN790 4.5 89.6 1.0 OE1 A:GLU901 4.5 73.0 1.0 CA A:GLU901 4.5 50.9 1.0 N A:ASN790 4.5 96.5 1.0 CB A:GLU901 4.5 55.4 1.0 N A:GLY786 4.6 77.0 1.0 CA A:VAL783 4.6 55.1 1.0 C A:VAL783 4.7 59.7 1.0 N A:VAL783 4.7 61.5 1.0 O A:VAL783 4.7 51.5 1.0 CA A:LYS782 4.7 55.0 1.0 N A:LEU788 4.8 93.0 1.0 N A:ASP785 4.9 66.8 1.0 C A:GLY786 4.9 81.8 1.0 CB A:LYS782 4.9 58.5 1.0 O A:ASN790 4.9 93.6 1.0 CB A:ASP785 4.9 62.1 1.0 CB A:PRO900 5.0 53.5 1.0

Y.C.Hsieh, H.H.Guan, C.C.Lin, T.Y.Huang, P.Chuankhayan, N.C.Chen, N.H.Wang, P.L.Hu, Y.C.Tsai, Y.C.Huang, M.Yoshimura, P.J.Lin, Y.H.Hsieh, C.J.Chen. Structure-Based High-Efficiency Homogeneous Antibody Platform By Endoglycosidase Sz Provides Insights Into Its Transglycosylation Mechanism Jacs Au V. 4 2130 2024.
ISSN: ESSN 2691-3704
DOI: 10.1021/JACSAU.4C00004