Calcium in PDB 8yrm: Iota Toxin Ib Pore Serine-Clamp Mutant

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 14;

Binding sites:

The binding sites of Calcium atom in the Iota Toxin Ib Pore Serine-Clamp Mutant (pdb code 8yrm). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 14 binding sites of Calcium where determined in the Iota Toxin Ib Pore Serine-Clamp Mutant, PDB code: 8yrm:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Calcium binding site 1 out of 14 in the Iota Toxin Ib Pore Serine-Clamp Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Iota Toxin Ib Pore Serine-Clamp Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca801 b:37.7 occ:1.00 O A:GLU262 2.2 40.7 1.0 OD2 A:ASP221 2.3 51.8 1.0 O A:SER259 2.3 42.5 1.0 OD2 A:ASP272 2.4 41.4 1.0 OE1 A:GLU230 2.5 41.2 1.0 OD2 A:ASP223 2.6 47.9 1.0 OE2 A:GLU230 2.8 44.3 1.0 CD A:GLU230 3.0 41.9 1.0 C A:GLU262 3.4 39.7 1.0 CG A:ASP221 3.4 48.8 1.0 CG A:ASP223 3.5 48.0 1.0 CG A:ASP272 3.5 38.2 1.0 C A:SER259 3.5 45.0 1.0 OD1 A:ASP223 3.9 49.2 1.0 CA A:SER263 4.0 38.3 1.0 OD1 A:ASP221 4.0 60.0 1.0 N A:SER263 4.1 38.1 1.0 CB A:ASP272 4.1 37.3 1.0 CA A:CA802 4.1 48.1 1.0 OG1 A:THR265 4.2 39.4 1.0 CA A:TYR260 4.2 47.3 1.0 N A:GLU262 4.3 44.3 1.0 N A:TYR260 4.3 44.0 1.0 CA A:GLU262 4.4 41.9 1.0 O A:SER263 4.4 43.2 1.0 C A:SER263 4.4 37.8 1.0 OD1 A:ASP272 4.4 42.0 1.0 N A:SER259 4.5 40.1 1.0 CG A:GLU230 4.5 41.2 1.0 CA A:SER259 4.5 43.9 1.0 C A:TYR260 4.5 44.0 1.0 OG A:SER258 4.5 45.7 1.0 CB A:ASP223 4.6 49.0 1.0 CB A:ASP221 4.6 49.4 1.0 CB A:SER258 4.6 42.2 1.0 O A:TYR260 4.9 49.4 1.0 N A:LEU261 4.9 40.5 1.0 C A:SER258 5.0 39.1 1.0

Calcium binding site 2 out of 14 in the Iota Toxin Ib Pore Serine-Clamp Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Iota Toxin Ib Pore Serine-Clamp Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca802 b:48.1 occ:1.00 OD1 A:ASP221 2.1 60.0 1.0 OD1 A:ASP223 2.1 49.2 1.0 OE2 A:GLU230 2.3 44.3 1.0 OD1 A:ASP219 2.3 60.0 1.0 O A:ILE225 2.3 45.8 1.0 CG A:ASP221 3.0 48.8 1.0 CG A:ASP223 3.2 48.0 1.0 OD2 A:ASP221 3.3 51.8 1.0 CD A:GLU230 3.4 41.9 1.0 CG A:ASP219 3.5 56.0 1.0 C A:ILE225 3.5 49.1 1.0 OD2 A:ASP223 3.7 47.9 1.0 OD1 A:ASP227 4.0 66.3 1.0 N A:ILE225 4.1 48.8 1.0 CA A:CA801 4.1 37.7 1.0 CG A:GLU230 4.1 41.2 1.0 CA A:ILE225 4.2 47.5 1.0 OD2 A:ASP219 4.2 62.0 1.0 N A:ASP223 4.2 49.9 1.0 CB A:ILE225 4.3 49.1 1.0 CA A:ASP219 4.4 54.7 1.0 O A:TYR260 4.4 49.4 1.0 N A:ASP221 4.4 46.1 1.0 CB A:ASP223 4.4 49.0 1.0 OE1 A:GLU230 4.4 41.2 1.0 CB A:ASP221 4.4 49.4 1.0 CB A:ASP219 4.5 56.5 1.0 C A:PRO226 4.5 52.9 1.0 N A:PRO226 4.6 53.3 1.0 N A:ASN222 4.6 52.7 1.0 CA A:TYR260 4.6 47.3 1.0 O A:PRO226 4.7 56.8 1.0 C A:ASP219 4.7 56.0 1.0 N A:THR220 4.7 51.4 1.0 CA A:ASP223 4.7 49.5 1.0 N A:ASP227 4.7 53.8 1.0 CA A:PRO226 4.8 51.9 1.0 CA A:ASP221 4.8 47.7 1.0 C A:ASP223 4.9 49.6 1.0 C A:TYR260 4.9 44.0 1.0 C A:ASP221 4.9 49.3 1.0 N A:ASN224 5.0 49.0 1.0

Calcium binding site 3 out of 14 in the Iota Toxin Ib Pore Serine-Clamp Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Iota Toxin Ib Pore Serine-Clamp Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca801 b:37.5 occ:1.00 O B:GLU262 2.2 40.5 1.0 OD2 B:ASP221 2.3 52.0 1.0 O B:SER259 2.3 42.2 1.0 OD2 B:ASP272 2.4 41.5 1.0 OE1 B:GLU230 2.5 41.5 1.0 OD2 B:ASP223 2.6 48.4 1.0 OE2 B:GLU230 2.8 43.9 1.0 CD B:GLU230 3.0 41.9 1.0 C B:GLU262 3.4 39.6 1.0 CG B:ASP221 3.4 48.6 1.0 CG B:ASP223 3.5 47.7 1.0 CG B:ASP272 3.5 38.0 1.0 C B:SER259 3.5 45.2 1.0 OD1 B:ASP223 3.9 49.1 1.0 CA B:SER263 4.0 38.4 1.0 OD1 B:ASP221 4.0 59.8 1.0 N B:SER263 4.1 38.0 1.0 CB B:ASP272 4.1 37.1 1.0 CA B:CA802 4.1 48.2 1.0 OG1 B:THR265 4.2 39.2 1.0 CA B:TYR260 4.2 47.0 1.0 N B:GLU262 4.3 44.3 1.0 N B:TYR260 4.3 44.3 1.0 O B:SER263 4.4 43.2 1.0 C B:SER263 4.4 37.8 1.0 CA B:GLU262 4.4 41.9 1.0 OD1 B:ASP272 4.4 41.8 1.0 N B:SER259 4.5 40.0 1.0 CG B:GLU230 4.5 41.0 1.0 OG B:SER258 4.5 46.0 1.0 C B:TYR260 4.5 43.9 1.0 CA B:SER259 4.5 44.0 1.0 CB B:ASP223 4.6 49.0 1.0 CB B:ASP221 4.6 49.3 1.0 CB B:SER258 4.6 42.5 1.0 O B:TYR260 4.9 48.8 1.0 N B:LEU261 4.9 40.6 1.0

Calcium binding site 4 out of 14 in the Iota Toxin Ib Pore Serine-Clamp Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Iota Toxin Ib Pore Serine-Clamp Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca802 b:48.2 occ:1.00 OD1 B:ASP221 2.1 59.8 1.0 OD1 B:ASP223 2.1 49.1 1.0 OD1 B:ASP219 2.3 60.5 1.0 OE2 B:GLU230 2.3 43.9 1.0 O B:ILE225 2.3 46.3 1.0 CG B:ASP221 3.0 48.6 1.0 CG B:ASP223 3.2 47.7 1.0 OD2 B:ASP221 3.4 52.0 1.0 CG B:ASP219 3.4 55.9 1.0 CD B:GLU230 3.5 41.9 1.0 C B:ILE225 3.5 49.1 1.0 OD2 B:ASP223 3.7 48.4 1.0 OD1 B:ASP227 4.0 66.0 1.0 N B:ILE225 4.0 48.7 1.0 CA B:CA801 4.1 37.5 1.0 CA B:ILE225 4.2 47.2 1.0 CG B:GLU230 4.2 41.0 1.0 OD2 B:ASP219 4.2 61.3 1.0 N B:ASP223 4.2 50.3 1.0 CB B:ILE225 4.3 48.8 1.0 CA B:ASP219 4.4 54.6 1.0 CB B:ASP223 4.4 49.0 1.0 N B:ASP221 4.4 46.3 1.0 O B:TYR260 4.4 48.8 1.0 OE1 B:GLU230 4.4 41.5 1.0 CB B:ASP221 4.4 49.3 1.0 CB B:ASP219 4.4 56.1 1.0 C B:PRO226 4.5 53.0 1.0 N B:PRO226 4.6 53.3 1.0 N B:ASN222 4.6 52.8 1.0 CA B:TYR260 4.7 47.0 1.0 O B:PRO226 4.7 57.2 1.0 C B:ASP219 4.7 55.9 1.0 CA B:ASP223 4.7 49.7 1.0 N B:THR220 4.7 51.7 1.0 N B:ASP227 4.7 53.9 1.0 CA B:PRO226 4.8 51.8 1.0 CA B:ASP221 4.8 47.8 1.0 C B:ASP223 4.8 49.9 1.0 C B:ASP221 4.9 49.3 1.0 C B:TYR260 4.9 43.9 1.0 N B:ASN224 4.9 49.0 1.0

Calcium binding site 5 out of 14 in the Iota Toxin Ib Pore Serine-Clamp Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Iota Toxin Ib Pore Serine-Clamp Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca801 b:37.4 occ:1.00 O C:GLU262 2.2 40.4 1.0 OD2 C:ASP221 2.3 51.9 1.0 O C:SER259 2.3 42.4 1.0 OD2 C:ASP272 2.4 41.2 1.0 OE1 C:GLU230 2.5 41.6 1.0 OD2 C:ASP223 2.6 48.2 1.0 OE2 C:GLU230 2.7 44.2 1.0 CD C:GLU230 3.0 41.8 1.0 C C:GLU262 3.4 39.6 1.0 CG C:ASP221 3.4 48.5 1.0 CG C:ASP223 3.5 47.8 1.0 CG C:ASP272 3.5 38.1 1.0 C C:SER259 3.5 45.1 1.0 OD1 C:ASP223 3.9 49.0 1.0 CA C:SER263 4.0 38.3 1.0 OD1 C:ASP221 4.0 59.7 1.0 N C:SER263 4.1 38.1 1.0 CB C:ASP272 4.1 37.1 1.0 CA C:CA802 4.1 48.2 1.0 OG1 C:THR265 4.2 39.1 1.0 CA C:TYR260 4.2 47.1 1.0 N C:GLU262 4.3 44.2 1.0 N C:TYR260 4.3 44.2 1.0 O C:SER263 4.4 43.1 1.0 C C:SER263 4.4 37.9 1.0 CA C:GLU262 4.4 41.9 1.0 OD1 C:ASP272 4.4 42.2 1.0 N C:SER259 4.5 40.1 1.0 CG C:GLU230 4.5 40.9 1.0 OG C:SER258 4.5 45.6 1.0 C C:TYR260 4.5 43.8 1.0 CA C:SER259 4.5 43.8 1.0 CB C:ASP223 4.6 48.8 1.0 CB C:ASP221 4.6 49.3 1.0 CB C:SER258 4.6 42.3 1.0 O C:TYR260 4.9 48.9 1.0 N C:LEU261 4.9 40.6 1.0 C C:SER258 5.0 39.0 1.0

Calcium binding site 6 out of 14 in the Iota Toxin Ib Pore Serine-Clamp Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Iota Toxin Ib Pore Serine-Clamp Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca802 b:48.2 occ:1.00 OD1 C:ASP221 2.1 59.7 1.0 OD1 C:ASP223 2.1 49.0 1.0 OD1 C:ASP219 2.3 60.4 1.0 OE2 C:GLU230 2.3 44.2 1.0 O C:ILE225 2.3 46.1 1.0 CG C:ASP221 3.1 48.5 1.0 CG C:ASP223 3.2 47.8 1.0 OD2 C:ASP221 3.4 51.9 1.0 CG C:ASP219 3.4 55.9 1.0 CD C:GLU230 3.5 41.8 1.0 C C:ILE225 3.5 49.0 1.0 OD2 C:ASP223 3.7 48.2 1.0 OD1 C:ASP227 4.0 66.1 1.0 N C:ILE225 4.0 48.8 1.0 CA C:CA801 4.1 37.4 1.0 CA C:ILE225 4.1 47.2 1.0 CG C:GLU230 4.2 40.9 1.0 OD2 C:ASP219 4.2 61.4 1.0 N C:ASP223 4.2 50.0 1.0 CB C:ILE225 4.3 48.8 1.0 CA C:ASP219 4.4 54.6 1.0 CB C:ASP223 4.4 48.8 1.0 N C:ASP221 4.4 46.2 1.0 O C:TYR260 4.4 48.9 1.0 OE1 C:GLU230 4.4 41.6 1.0 CB C:ASP221 4.4 49.3 1.0 CB C:ASP219 4.4 56.2 1.0 C C:PRO226 4.5 52.8 1.0 N C:PRO226 4.6 53.3 1.0 N C:ASN222 4.6 52.7 1.0 O C:PRO226 4.7 57.1 1.0 CA C:TYR260 4.7 47.1 1.0 C C:ASP219 4.7 55.9 1.0 N C:THR220 4.7 51.6 1.0 CA C:ASP223 4.7 49.5 1.0 N C:ASP227 4.7 53.7 1.0 CA C:PRO226 4.8 51.8 1.0 CA C:ASP221 4.8 47.8 1.0 C C:ASP223 4.9 49.7 1.0 C C:TYR260 4.9 43.8 1.0 C C:ASP221 4.9 49.2 1.0 N C:ASN224 4.9 48.9 1.0

Calcium binding site 7 out of 14 in the Iota Toxin Ib Pore Serine-Clamp Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Iota Toxin Ib Pore Serine-Clamp Mutant within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca801 b:37.4 occ:1.00 O D:GLU262 2.2 40.5 1.0 OD2 D:ASP221 2.3 51.9 1.0 O D:SER259 2.3 42.3 1.0 OD2 D:ASP272 2.4 41.5 1.0 OE1 D:GLU230 2.5 41.6 1.0 OD2 D:ASP223 2.6 48.5 1.0 OE2 D:GLU230 2.7 44.0 1.0 CD D:GLU230 3.0 41.9 1.0 C D:GLU262 3.4 39.6 1.0 CG D:ASP221 3.4 48.5 1.0 CG D:ASP223 3.5 47.7 1.0 CG D:ASP272 3.5 38.0 1.0 C D:SER259 3.5 45.2 1.0 OD1 D:ASP223 3.9 48.9 1.0 CA D:SER263 4.0 38.4 1.0 OD1 D:ASP221 4.0 59.7 1.0 N D:SER263 4.1 38.1 1.0 CB D:ASP272 4.1 37.1 1.0 CA D:CA802 4.1 48.2 1.0 OG1 D:THR265 4.2 39.1 1.0 CA D:TYR260 4.2 47.1 1.0 N D:GLU262 4.3 44.2 1.0 N D:TYR260 4.3 44.4 1.0 O D:SER263 4.4 43.0 1.0 C D:SER263 4.4 37.9 1.0 CA D:GLU262 4.4 42.0 1.0 OD1 D:ASP272 4.4 42.0 1.0 N D:SER259 4.5 40.1 1.0 CG D:GLU230 4.5 40.9 1.0 OG D:SER258 4.5 45.8 1.0 C D:TYR260 4.5 43.8 1.0 CA D:SER259 4.6 43.9 1.0 CB D:ASP223 4.6 48.9 1.0 CB D:ASP221 4.6 49.2 1.0 CB D:SER258 4.6 42.4 1.0 O D:TYR260 4.9 48.7 1.0 N D:LEU261 4.9 40.6 1.0

Calcium binding site 8 out of 14 in the Iota Toxin Ib Pore Serine-Clamp Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 8 of Iota Toxin Ib Pore Serine-Clamp Mutant within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca802 b:48.2 occ:1.00 OD1 D:ASP221 2.1 59.7 1.0 OD1 D:ASP223 2.1 48.9 1.0 OD1 D:ASP219 2.2 60.6 1.0 OE2 D:GLU230 2.3 44.0 1.0 O D:ILE225 2.3 46.2 1.0 CG D:ASP221 3.1 48.5 1.0 CG D:ASP223 3.2 47.7 1.0 OD2 D:ASP221 3.4 51.9 1.0 CG D:ASP219 3.4 55.9 1.0 CD D:GLU230 3.5 41.9 1.0 C D:ILE225 3.5 49.1 1.0 OD2 D:ASP223 3.7 48.5 1.0 OD1 D:ASP227 4.0 66.1 1.0 N D:ILE225 4.0 48.8 1.0 CA D:CA801 4.1 37.4 1.0 CA D:ILE225 4.1 47.2 1.0 CG D:GLU230 4.2 40.9 1.0 OD2 D:ASP219 4.2 61.2 1.0 N D:ASP223 4.2 50.2 1.0 CB D:ILE225 4.3 48.8 1.0 CA D:ASP219 4.4 54.5 1.0 CB D:ASP223 4.4 48.9 1.0 N D:ASP221 4.4 46.2 1.0 O D:TYR260 4.4 48.7 1.0 OE1 D:GLU230 4.4 41.6 1.0 CB D:ASP221 4.4 49.2 1.0 CB D:ASP219 4.4 56.0 1.0 C D:PRO226 4.5 53.0 1.0 N D:PRO226 4.6 53.3 1.0 N D:ASN222 4.6 52.8 1.0 O D:PRO226 4.7 57.4 1.0 CA D:TYR260 4.7 47.1 1.0 C D:ASP219 4.7 55.7 1.0 CA D:ASP223 4.7 49.6 1.0 N D:THR220 4.7 51.6 1.0 N D:ASP227 4.7 53.9 1.0 CA D:PRO226 4.8 51.9 1.0 CA D:ASP221 4.8 47.7 1.0 C D:ASP223 4.8 49.8 1.0 C D:ASP221 4.9 49.2 1.0 C D:TYR260 4.9 43.8 1.0 N D:ASN224 4.9 48.9 1.0

Calcium binding site 9 out of 14 in the Iota Toxin Ib Pore Serine-Clamp Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 9 of Iota Toxin Ib Pore Serine-Clamp Mutant within 5.0Å range: probe atom residue distance (Å) B Occ E:Ca801 b:37.5 occ:1.00 O E:GLU262 2.2 40.5 1.0 OD2 E:ASP221 2.3 52.1 1.0 O E:SER259 2.3 42.4 1.0 OD2 E:ASP272 2.4 41.2 1.0 OE1 E:GLU230 2.5 41.4 1.0 OD2 E:ASP223 2.6 48.3 1.0 OE2 E:GLU230 2.7 43.9 1.0 CD E:GLU230 3.0 41.8 1.0 C E:GLU262 3.4 39.6 1.0 CG E:ASP221 3.4 48.5 1.0 CG E:ASP223 3.5 47.6 1.0 CG E:ASP272 3.5 38.0 1.0 C E:SER259 3.5 45.2 1.0 OD1 E:ASP223 3.9 48.7 1.0 CA E:SER263 4.0 38.3 1.0 OD1 E:ASP221 4.0 59.6 1.0 N E:SER263 4.1 38.0 1.0 CB E:ASP272 4.1 37.1 1.0 CA E:CA802 4.1 48.1 1.0 OG1 E:THR265 4.2 39.3 1.0 CA E:TYR260 4.2 47.1 1.0 N E:GLU262 4.3 44.2 1.0 N E:TYR260 4.3 44.3 1.0 O E:SER263 4.4 43.2 1.0 C E:SER263 4.4 37.7 1.0 CA E:GLU262 4.4 41.8 1.0 OD1 E:ASP272 4.4 42.0 1.0 N E:SER259 4.5 40.0 1.0 CG E:GLU230 4.5 40.9 1.0 C E:TYR260 4.5 43.9 1.0 OG E:SER258 4.5 45.8 1.0 CA E:SER259 4.5 43.8 1.0 CB E:ASP223 4.6 48.9 1.0 CB E:ASP221 4.6 49.3 1.0 CB E:SER258 4.6 42.4 1.0 O E:TYR260 4.9 48.8 1.0 N E:LEU261 4.9 40.6 1.0

Calcium binding site 10 out of 14 in the Iota Toxin Ib Pore Serine-Clamp Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 10 of Iota Toxin Ib Pore Serine-Clamp Mutant within 5.0Å range: probe atom residue distance (Å) B Occ E:Ca802 b:48.1 occ:1.00 OD1 E:ASP221 2.1 59.6 1.0 OD1 E:ASP223 2.1 48.7 1.0 OD1 E:ASP219 2.3 60.4 1.0 OE2 E:GLU230 2.3 43.9 1.0 O E:ILE225 2.3 45.9 1.0 CG E:ASP221 3.0 48.5 1.0 CG E:ASP223 3.2 47.6 1.0 OD2 E:ASP221 3.4 52.1 1.0 CG E:ASP219 3.4 56.0 1.0 CD E:GLU230 3.5 41.8 1.0 C E:ILE225 3.5 49.0 1.0 OD2 E:ASP223 3.7 48.3 1.0 OD1 E:ASP227 4.0 66.0 1.0 N E:ILE225 4.1 48.7 1.0 CA E:CA801 4.1 37.5 1.0 CA E:ILE225 4.2 47.2 1.0 CG E:GLU230 4.2 40.9 1.0 OD2 E:ASP219 4.2 61.8 1.0 N E:ASP223 4.2 50.2 1.0 CB E:ILE225 4.3 48.8 1.0 CA E:ASP219 4.4 54.7 1.0 N E:ASP221 4.4 46.3 1.0 CB E:ASP223 4.4 48.9 1.0 O E:TYR260 4.4 48.8 1.0 OE1 E:GLU230 4.4 41.4 1.0 CB E:ASP221 4.4 49.3 1.0 CB E:ASP219 4.4 56.1 1.0 C E:PRO226 4.5 52.9 1.0 N E:PRO226 4.6 53.2 1.0 N E:ASN222 4.6 52.8 1.0 CA E:TYR260 4.7 47.1 1.0 O E:PRO226 4.7 57.3 1.0 C E:ASP219 4.7 56.0 1.0 N E:THR220 4.7 51.7 1.0 CA E:ASP223 4.7 49.5 1.0 N E:ASP227 4.7 53.8 1.0 CA E:PRO226 4.8 51.7 1.0 CA E:ASP221 4.8 47.8 1.0 C E:ASP223 4.9 49.8 1.0 C E:TYR260 4.9 43.9 1.0 C E:ASP221 4.9 49.3 1.0 N E:ASN224 4.9 48.9 1.0

T.Yoshida, C.Monma, Y.Mitani, S.Takiguchi, S.Fujita, Y.Uchida, V.A.Karginov, T.Yamada, J.Kishikawa, R.Kawano, H.Tsuge. Serine Clamp of Clostridium Perfringens Binary Toxin Cpileb-Pore Confers Cytotoxicity and Enterotoxicity To Be Published.