Calcium in PDB 9cyj: Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2)

All present enzymatic activity of Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2):
3.2.1.18;

The binding sites of Calcium atom in the Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2) (pdb code 9cyj). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2), PDB code: 9cyj:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2) within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca501 b:91.8 occ:1.00 O C:GLY297 2.4 86.0 1.0 O C:GLY346 3.2 93.8 1.0 O C:LYS344 3.5 91.3 1.0 O C:ASP293 3.6 94.9 1.0 C C:GLY297 3.6 80.3 1.0 O C:ASP324 3.8 75.0 1.0 OD2 C:ASP324 3.8 73.1 1.0 CG C:ASP324 4.0 70.1 1.0 N C:GLY348 4.1 81.9 1.0 CA C:GLY348 4.2 77.5 1.0 OD1 C:ASP324 4.2 73.9 1.0 CA C:SER298 4.3 76.0 1.0 C C:GLY346 4.3 91.9 1.0 N C:SER298 4.4 78.3 1.0 O C:ASN294 4.4 101.8 1.0 N C:GLY346 4.5 98.0 1.0 CB C:ASP324 4.6 65.8 1.0 N C:GLY297 4.6 80.9 1.0 CA C:GLY297 4.7 81.9 1.0 C C:LYS344 4.7 90.7 1.0 O C:PRO326 4.7 82.4 1.0 C C:ASP293 4.8 94.2 1.0 C C:ASN294 4.8 97.5 1.0 CB C:SER298 4.8 77.6 1.0 C C:HIS347 4.9 97.4 1.0 C C:ASP324 4.9 68.4 1.0 CA C:GLY346 4.9 94.7 1.0

Calcium binding site 2 out of 4 in the Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2) within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca501 b:90.5 occ:1.00 O B:GLY297 3.1 85.1 1.0 O B:GLY346 3.4 94.2 1.0 O B:LYS344 3.4 91.1 1.0 OD2 B:ASP324 3.7 73.2 1.0 C B:GLY297 3.9 80.2 1.0 CG B:ASP324 4.0 69.7 1.0 CA B:SER298 4.1 76.2 1.0 O B:ASP293 4.1 94.1 1.0 CA B:GLY348 4.2 77.7 1.0 OD1 B:ASP324 4.3 73.6 1.0 N B:SER298 4.4 79.4 1.0 N B:GLY348 4.4 81.7 1.0 C B:GLY346 4.4 91.8 1.0 O B:PRO326 4.4 83.1 1.0 CB B:SER298 4.5 77.5 1.0 N B:GLY346 4.5 97.9 1.0 C B:LYS344 4.5 90.4 1.0 CB B:ASP324 4.6 65.6 1.0 C B:GLY345 4.6 95.3 1.0 O B:THR325 4.6 75.8 1.0 CA B:GLY345 4.7 93.4 1.0 CD B:PRO326 4.8 79.6 1.0 C B:THR325 4.9 71.6 1.0 N B:GLY297 4.9 80.2 1.0 N B:PRO326 4.9 73.8 1.0 CA B:GLY346 5.0 94.2 1.0

Calcium binding site 3 out of 4 in the Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca501 b:88.4 occ:1.00 O A:GLY345 2.6 89.5 1.0 O A:ASP293 2.8 95.2 1.0 O A:GLY297 3.3 85.8 1.0 N A:GLY348 3.6 82.3 1.0 CA A:GLY348 3.6 77.8 1.0 O A:LYS344 3.7 89.2 1.0 C A:GLY345 3.8 94.2 1.0 OD2 A:ASP324 3.9 72.8 1.0 O A:ASN294 3.9 101.5 1.0 C A:ASP293 4.1 94.5 1.0 CG A:ASP324 4.1 69.6 1.0 CB A:ASP324 4.2 66.2 1.0 C A:GLY297 4.2 80.8 1.0 C A:HIS347 4.2 97.0 1.0 C A:ASN294 4.4 97.0 1.0 N A:HIS347 4.5 94.7 1.0 CA A:GLY345 4.5 91.7 1.0 CA A:SER298 4.5 74.8 1.0 CA A:ASN294 4.7 95.7 1.0 C A:GLY346 4.7 91.1 1.0 N A:SER298 4.7 78.4 1.0 CA A:HIS347 4.8 94.8 1.0 O A:HIS347 4.8 98.2 1.0 C A:LYS344 4.8 89.1 1.0 N A:GLY346 4.8 96.6 1.0 O A:PRO326 4.9 82.2 1.0 N A:ASN294 4.9 93.3 1.0 N A:GLY297 4.9 80.8 1.0 OD1 A:ASP324 4.9 72.8 1.0 OG A:SER298 5.0 77.5 1.0 CA A:GLY346 5.0 93.2 1.0

Calcium binding site 4 out of 4 in the Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Cryo-Em Structure of FNI9 Igg in Complex with Influenza Virus Neuraminidase From A/Kansas/14/2017 (H3N2) within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca501 b:88.8 occ:1.00 O D:GLY346 3.4 92.6 1.0 O D:LYS344 3.6 90.4 1.0 O D:GLY297 3.6 86.0 1.0 OD2 D:ASP324 3.9 71.9 1.0 O D:TRP295 4.0 93.1 1.0 CG D:ASP324 4.1 68.9 1.0 CA D:GLY348 4.1 76.7 1.0 O D:ASP293 4.3 95.2 1.0 C D:GLY297 4.3 80.8 1.0 N D:GLY348 4.3 81.8 1.0 OD1 D:ASP324 4.4 72.6 1.0 O D:GLY345 4.4 90.4 1.0 CA D:SER298 4.5 75.4 1.0 O D:PRO326 4.5 82.0 1.0 C D:GLY346 4.6 91.9 1.0 C D:GLY345 4.6 94.2 1.0 CD D:PRO326 4.6 79.5 1.0 O D:ASP324 4.6 74.8 1.0 C D:LYS344 4.6 89.8 1.0 N D:SER298 4.7 78.5 1.0 CB D:ASP324 4.7 65.8 1.0 O D:THR325 4.7 76.2 1.0 C D:TRP295 4.7 95.9 1.0 C D:HIS347 4.8 97.8 1.0 O D:ASN294 4.9 102.5 1.0

G.Jo, S.Yamayoshi, K.M.Ma, O.Swanson, J.L.Torres, J.A.Ferguson, M.L.Fernandez-Quintero, J.Huang, J.Copps, A.J.Rodriguez, J.M.Steichen, Y.Kawaoka, J.Han, A.B.Ward. Structural Basis of Broad Protection Against Influenza Virus By Human Antibodies Targeting the Neuraminidase Active Site Via A Recurring Motif in Cdr H3. Nat Commun V. 16 7067 2025.
ISSN: ESSN 2041-1723
PubMed: 40750588
DOI: 10.1038/S41467-025-62174-2