Calcium in PDB 1bn8: Bacillus Subtilis Pectate Lyase

All present enzymatic activity of Bacillus Subtilis Pectate Lyase:
4.2.2.2;

The structure of Bacillus Subtilis Pectate Lyase, PDB code: 1bn8 was solved by R.Pickersgill, G.Harris, J.Jenkins, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	55.900, 89.000, 50.700, 90.00, 108.93, 90.00
R / Rfree (%)	17.9 / n/a

The binding sites of Calcium atom in the Bacillus Subtilis Pectate Lyase (pdb code 1bn8). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Bacillus Subtilis Pectate Lyase, PDB code: 1bn8:

Calcium binding site 1 out of 1 in the Bacillus Subtilis Pectate Lyase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Bacillus Subtilis Pectate Lyase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca400 b:6.5 occ:1.00 OD2 A:ASP223 2.8 12.9 1.0 OD2 A:ASP227 2.8 11.2 1.0 OD1 A:ASP184 2.8 7.9 1.0 OD2 A:ASP184 2.9 8.8 1.0 O A:HOH442 3.1 34.5 1.0 O A:HOH444 3.2 40.5 1.0 CG A:ASP184 3.2 8.1 1.0 CG A:ASP227 3.8 9.3 1.0 CG A:ASP223 3.9 11.1 1.0 CB A:ASP227 4.0 8.3 1.0 N A:GLY224 4.3 6.5 1.0 O A:HOH539 4.4 31.1 1.0 CB A:ASP223 4.4 9.2 1.0 O A:HOH489 4.5 22.9 1.0 CB A:ASP184 4.7 7.4 1.0 OD1 A:ASP223 4.7 12.0 1.0 CA A:GLY224 4.8 5.8 1.0 OD1 A:ASP227 4.9 10.1 1.0 O A:HOH455 4.9 17.8 1.0

R.Pickersgill, J.Jenkins, G.Harris, W.Nasser, J.Robert-Baudouy. The Structure of Bacillus Subtilis Pectate Lyase in Complex with Calcium. Nat.Struct.Biol. V. 1 717 1994.
ISSN: ISSN 1072-8368
PubMed: 7634076
DOI: 10.1038/NSB1094-717