Calcium in PDB 1bss: Ecorv-T93A/Dna/CA2+

All present enzymatic activity of Ecorv-T93A/Dna/CA2+:
3.1.21.4;

The structure of Ecorv-T93A/Dna/CA2+, PDB code: 1bss was solved by J.J.Perona, N.C.Horton, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 2.15
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	50.000, 64.400, 49.200, 109.20, 108.70, 95.80
R / Rfree (%)	18.4 / 24.6

The binding sites of Calcium atom in the Ecorv-T93A/Dna/CA2+ (pdb code 1bss). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Ecorv-T93A/Dna/CA2+, PDB code: 1bss:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Ecorv-T93A/Dna/CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ecorv-T93A/Dna/CA2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca433 b:40.2 occ:1.00 O A:HOH514 2.2 35.5 1.0 O A:HOH510 2.2 35.5 1.0 OE2 A:GLU45 2.3 38.4 1.0 OD1 A:ASP74 2.3 27.8 1.0 OE1 A:GLU45 2.8 37.4 1.0 CD A:GLU45 2.8 36.0 1.0 CG A:ASP74 3.1 22.2 1.0 CB A:ASP74 3.4 21.9 1.0 CA A:ASP74 3.7 24.5 1.0 O A:HOH497 4.1 31.4 1.0 OD2 A:ASP74 4.2 22.9 1.0 CG A:GLU45 4.3 34.2 1.0 OE1 A:GLU65 4.4 50.1 1.0 O A:ASP74 4.4 24.7 1.0 C A:ASP74 4.6 24.1 1.0 O A:HOH511 4.7 31.6 1.0 N A:ASP74 4.8 23.7 1.0

Calcium binding site 2 out of 2 in the Ecorv-T93A/Dna/CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Ecorv-T93A/Dna/CA2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca436 b:46.3 occ:1.00 O A:HOH513 2.2 33.4 1.0 O A:ILE91 2.3 26.6 1.0 OD2 A:ASP74 2.4 22.9 1.0 O C:HOH437 2.6 29.2 1.0 O A:HOH511 2.6 31.6 1.0 OD1 A:ASP90 3.1 29.2 1.0 CG A:ASP74 3.3 22.2 1.0 C A:ILE91 3.4 23.8 1.0 OD1 A:ASP74 3.4 27.8 1.0 N A:ILE91 3.7 23.9 1.0 CG A:ASP90 3.9 27.7 1.0 CA A:ILE91 4.0 24.8 1.0 CB A:ILE91 4.3 24.9 1.0 OD2 A:ASP90 4.3 31.7 1.0 OE1 A:GLU45 4.3 37.4 1.0 C A:ASP90 4.4 22.2 1.0 OP1 C:DA907 4.4 39.2 1.0 N A:LYS92 4.5 22.4 1.0 OP1 C:DT908 4.7 27.5 1.0 CB A:ASP74 4.7 21.9 1.0 OG A:SER41 4.8 48.2 1.0 CA A:ASP90 4.8 23.7 1.0 CD A:LYS92 4.8 23.6 1.0 CA A:LYS92 4.9 21.8 1.0 O3' C:DA907 4.9 37.8 1.0 CB A:ASP90 4.9 28.0 1.0

N.C.Horton, K.J.Newberry, J.J.Perona. Metal Ion-Mediated Substrate-Assisted Catalysis in Type II Restriction Endonucleases Proc.Natl.Acad.Sci.Usa V. 95 13489 1998.
ISSN: ISSN 0027-8424
PubMed: 9811827
DOI: 10.1073/PNAS.95.23.13489