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Calcium in PDB 1bsu: Structural and Energetic Origins of Indirect Readout in Site-Specific Dna Cleavage By A Restriction Endonuclease

Enzymatic activity of Structural and Energetic Origins of Indirect Readout in Site-Specific Dna Cleavage By A Restriction Endonuclease

All present enzymatic activity of Structural and Energetic Origins of Indirect Readout in Site-Specific Dna Cleavage By A Restriction Endonuclease:
3.1.21.4;

Protein crystallography data

The structure of Structural and Energetic Origins of Indirect Readout in Site-Specific Dna Cleavage By A Restriction Endonuclease, PDB code: 1bsu was solved by J.J.Perona, A.M.Martin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 6.00 / 2.00
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 50.300, 64.000, 49.100, 109.10, 108.10, 96.50
R / Rfree (%) 18.3 / 25.8

Calcium Binding Sites:

The binding sites of Calcium atom in the Structural and Energetic Origins of Indirect Readout in Site-Specific Dna Cleavage By A Restriction Endonuclease (pdb code 1bsu). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structural and Energetic Origins of Indirect Readout in Site-Specific Dna Cleavage By A Restriction Endonuclease, PDB code: 1bsu:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 1bsu

Go back to Calcium Binding Sites List in 1bsu
Calcium binding site 1 out of 2 in the Structural and Energetic Origins of Indirect Readout in Site-Specific Dna Cleavage By A Restriction Endonuclease


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structural and Energetic Origins of Indirect Readout in Site-Specific Dna Cleavage By A Restriction Endonuclease within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca301

b:30.6
occ:1.00
O A:HOH397 2.2 23.0 1.0
OD1 A:ASP74 2.3 18.1 1.0
O A:HOH388 2.3 24.7 1.0
OP1 C:DI907 2.4 27.2 1.0
OD2 A:ASP90 2.6 20.7 1.0
O A:HOH389 2.6 26.7 1.0
OD1 A:ASP90 2.6 13.9 1.0
OD2 A:ASP74 2.8 20.9 1.0
CG A:ASP74 2.8 13.9 1.0
CG A:ASP90 2.9 19.7 1.0
P C:DI907 3.7 16.7 1.0
O A:HOH399 3.8 46.6 1.0
C5' C:DI907 4.1 24.7 1.0
O3' C:5CM906 4.1 18.8 1.0
CB A:ASP74 4.3 15.1 1.0
N A:ASP74 4.3 15.3 1.0
O5' C:DI907 4.4 24.1 1.0
CB A:ASP90 4.4 15.5 1.0
NZ A:LYS92 4.4 15.7 1.0
O A:ILE91 4.6 15.9 1.0
CA A:ASP74 4.7 17.0 1.0
N A:ILE91 4.9 18.5 1.0
OP2 C:DI907 5.0 12.1 1.0
CA A:ASP90 5.0 15.5 1.0

Calcium binding site 2 out of 2 in 1bsu

Go back to Calcium Binding Sites List in 1bsu
Calcium binding site 2 out of 2 in the Structural and Energetic Origins of Indirect Readout in Site-Specific Dna Cleavage By A Restriction Endonuclease


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structural and Energetic Origins of Indirect Readout in Site-Specific Dna Cleavage By A Restriction Endonuclease within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca300

b:21.2
occ:1.00
O B:HOH388 2.0 9.2 1.0
O B:HOH392 2.3 23.5 1.0
OD1 B:ASP74 2.3 16.2 1.0
OP1 D:DI807 2.3 28.1 1.0
OD2 B:ASP90 2.6 17.7 1.0
O B:HOH375 2.6 18.8 1.0
OD1 B:ASP90 2.7 14.6 1.0
CG B:ASP90 2.9 10.4 1.0
CG B:ASP74 3.0 13.6 1.0
OD2 B:ASP74 3.1 15.0 1.0
P D:DI807 3.7 18.8 1.0
O3' D:5CM806 3.9 18.2 1.0
C5' D:DI807 4.1 19.4 1.0
N B:ASP74 4.2 15.4 1.0
CB B:ASP74 4.4 10.5 1.0
O5' D:DI807 4.4 21.8 1.0
CB B:ASP90 4.4 15.2 1.0
CA B:ASP74 4.6 16.0 1.0
NZ B:LYS92 4.7 15.4 1.0
O B:ILE91 4.7 18.5 1.0
O B:HOH359 4.7 40.8 1.0
C B:PRO73 4.9 11.8 1.0
OP2 D:DI807 4.9 21.1 1.0
CA B:ASP90 4.9 12.4 1.0
N B:ILE91 5.0 16.0 1.0

Reference:

A.M.Martin, M.D.Sam, N.O.Reich, J.J.Perona. Structural and Energetic Origins of Indirect Readout in Site-Specific Dna Cleavage By A Restriction Endonuclease. Nat.Struct.Biol. V. 6 269 1999.
ISSN: ISSN 1072-8368
PubMed: 10074946
DOI: 10.1038/6707
Page generated: Thu Jul 11 06:32:52 2024

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