Calcium in PDB 1fz9: Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane

All present enzymatic activity of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane:
1.14.13.25;

The structure of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane, PDB code: 1fz9 was solved by D.A.Whittington, A.C.Rosenzweig, C.A.Frederick, S.J.Lippard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	71.220, 172.220, 221.190, 90.00, 90.00, 90.00
R / Rfree (%)	20.6 / 26

The structure of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane also contains other interesting chemical elements:

Iodine	(I)	8 atoms
Iron	(Fe)	4 atoms

The binding sites of Calcium atom in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane (pdb code 1fz9). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane, PDB code: 1fz9:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca5005 b:55.9 occ:1.00 OXT A:ASN527 2.3 42.7 1.0 C A:ASN527 3.3 41.7 1.0 CA A:ASN527 3.7 43.7 1.0 NH1 E:ARG162 4.4 53.8 1.0 O A:ASN527 4.4 40.0 1.0 O A:PHE526 4.5 44.0 1.0 OE1 A:GLU440 4.5 58.0 1.0 CB A:ASN527 4.6 43.8 1.0 N A:ASN527 4.8 41.9 1.0 O A:HOH9056 4.9 30.3 1.0

Calcium binding site 2 out of 3 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca5006 b:88.1 occ:1.00 O C:HOH9146 2.5 52.1 1.0 OD2 C:ASP348 2.6 47.5 1.0 O C:HOH9008 2.7 37.1 1.0 O C:HOH9130 2.9 59.5 1.0 CG C:ASP348 3.5 42.7 1.0 OD1 C:ASP348 3.9 46.4 1.0 CB C:ASP348 4.7 38.0 1.0 OE1 C:GLU350 4.9 42.8 1.0 O C:HOH9199 4.9 36.0 1.0

Calcium binding site 3 out of 3 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Iodoethane within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca5007 b:57.9 occ:1.00 OE1 C:GLU222 2.6 37.0 1.0 CD C:GLU222 3.8 38.2 1.0 OD1 C:ASP334 4.3 33.8 1.0 CB C:GLU222 4.4 32.4 1.0 CG C:GLU222 4.5 31.9 1.0 OD2 C:ASP334 4.6 31.7 1.0 CG C:ASP334 4.6 28.1 1.0 CD C:LYS225 4.6 28.9 1.0 OE2 C:GLU222 4.8 42.8 1.0 O C:GLU222 4.9 32.5 1.0 O C:HOH9112 4.9 35.0 1.0 CA C:GLU222 4.9 30.5 1.0

D.A.Whittington, A.C.Rosenzweig, C.A.Frederick, S.J.Lippard. Xenon and Halogenated Alkanes Track Putative Substrate Binding Cavities in the Soluble Methane Monooxygenase Hydroxylase. Biochemistry V. 40 3476 2001.
ISSN: ISSN 0006-2960
PubMed: 11297413
DOI: 10.1021/BI0022487