Calcium in PDB 1g9h: Ternary Complex Between Psychrophilic Alpha-Amylase, Comii (Pseudo Tri-Saccharide From Bayer) and Tris (2-Amino-2-Hydroxymethyl-Propane- 1,3-Diol)

Enzymatic activity of Ternary Complex Between Psychrophilic Alpha-Amylase, Comii (Pseudo Tri-Saccharide From Bayer) and Tris (2-Amino-2-Hydroxymethyl-Propane- 1,3-Diol)

All present enzymatic activity of Ternary Complex Between Psychrophilic Alpha-Amylase, Comii (Pseudo Tri-Saccharide From Bayer) and Tris (2-Amino-2-Hydroxymethyl-Propane- 1,3-Diol):
3.2.1.1;

Protein crystallography data

The structure of Ternary Complex Between Psychrophilic Alpha-Amylase, Comii (Pseudo Tri-Saccharide From Bayer) and Tris (2-Amino-2-Hydroxymethyl-Propane- 1,3-Diol), PDB code: 1g9h was solved by N.Aghajari, M.Roth, R.Haser, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	44.28 / 1.80
*Space group*	C 2 2 2₁
*Cell size a, b, c (Å), α, β, γ (°)*	71.330, 138.680, 114.810, 90.00, 90.00, 90.00
*R / R_free (%)*	16.8 / 20.1

Other elements in 1g9h:

Chlorine

(Cl)

1 atom

Calcium Binding Sites:

The binding sites of Calcium atom in the Ternary Complex Between Psychrophilic Alpha-Amylase, Comii (Pseudo Tri-Saccharide From Bayer) and Tris (2-Amino-2-Hydroxymethyl-Propane- 1,3-Diol) (pdb code 1g9h). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Ternary Complex Between Psychrophilic Alpha-Amylase, Comii (Pseudo Tri-Saccharide From Bayer) and Tris (2-Amino-2-Hydroxymethyl-Propane- 1,3-Diol), PDB code: 1g9h:

Calcium binding site 1 out of 1 in 1g9h

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Calcium Binding Sites List in 1g9h

Calcium binding site 1 out of 1 in the Ternary Complex Between Psychrophilic Alpha-Amylase, Comii (Pseudo Tri-Saccharide From Bayer) and Tris (2-Amino-2-Hydroxymethyl-Propane- 1,3-Diol)

Mono view

Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ternary Complex Between Psychrophilic Alpha-Amylase, Comii (Pseudo Tri-Saccharide From Bayer) and Tris (2-Amino-2-Hydroxymethyl-Propane- 1,3-Diol) within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Ca800 b:14.8 occ:1.00	O	A:HIS178	2.4	13.7	1.0
	OD1	A:ASN88	2.4	16.7	1.0
	O	A:HOH1091	2.5	14.2	1.0
	O	A:HOH1107	2.5	13.5	1.0
	O	A:GLN135	2.5	17.3	1.0
	OD2	A:ASP144	2.5	14.6	1.0
	O	A:HOH1108	2.5	13.3	1.0
	OD1	A:ASP144	2.6	14.4	1.0
	CG	A:ASP144	2.9	13.4	1.0
	C	A:GLN135	3.5	16.0	1.0
	CG	A:ASN88	3.5	13.9	1.0
	C	A:HIS178	3.6	15.3	1.0
	ND2	A:ASN88	4.0	13.9	1.0
	CA	A:GLN135	4.1	14.9	1.0
	CB	A:HIS178	4.2	11.7	1.0
	N	A:ASN136	4.4	16.7	1.0
	O	A:ASN88	4.4	15.6	1.0
	CA	A:HIS178	4.4	12.8	1.0
	CB	A:ASP144	4.4	9.7	1.0
	CE1	A:HIS117	4.5	17.1	1.0
	ND1	A:HIS117	4.5	17.9	1.0
	CG2	A:VAL179	4.5	12.3	1.0
	N	A:VAL179	4.5	12.5	1.0
	O	A:LEU145	4.6	14.3	1.0
	CA	A:ASN136	4.6	17.6	1.0
	CA	A:VAL179	4.6	12.0	1.0
	O	A:CYS137	4.7	14.8	1.0
	CB	A:ASN88	4.8	15.4	1.0
	O	A:VAL134	4.9	13.5	1.0
	CB	A:GLN135	4.9	14.2	1.0
	O	A:HOH1144	4.9	18.6	1.0
	C	A:ASN136	5.0	17.0	1.0

Reference:

N.Aghajari, M.Roth, R.Haser. Crystallographic Evidence of A Transglycosylation Reaction: Ternary Complexes of A Psychrophilic Alpha-Amylase. Biochemistry V. 41 4273 2002.
ISSN: ISSN 0006-2960
PubMed: 11914073
DOI: 10.1021/BI0160516

Page generated: Mon Jul 7 15:11:40 2025

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